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CCNE1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS643796

Cyclin E1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and… More details

$940 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CCNE1, Polyclonal Antibody
Full Product Name
CCNE1, ID (G1/S-specific Cyclin-E1, CCNE)
Product Gene Name
anti-Ccne1 antibody
Product Synonym Names
Anti -CCNE1, ID (G1/S-specific Cyclin-E1, CCNE)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes human CCNE1. Species sequence homology: mouse and rat.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
47,482 Da
Chromosome Location
Chromosome: 1; NC_005100.3 (95575136..95584373, complement). Location: 1q22
3D Structure
ModBase 3D Structure for P39949
Immunogen
Synthetic peptide corresponding to human Cyclin E1.

Product Categories/Family for anti-Ccne1 antibody

Antibodies; Abs to Cyclin
Applications Tested/Suitable for anti-Ccne1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-Ccne1 antibody
Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
Dilution: ELISA: 1:20,000
Western Blot: 1:500-1:1000
Immunofluorescence: 1:100-1:500
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml

Testing Data of anti-Ccne1 antibody

Human Lung, Respiratory Epithelium
Rabbit Anti-CCNE1 Polyclonal Antibody – Cat# MBS643796

Western Blot (WB) of anti-Ccne1 antibody

Western blot analysis of extracts from K562 cells, using Cyclin E1 Antibody. The lane on the right is treated with the synthesized peptide.
Rabbit Anti-CCNE1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS643796

Immunofluorescence (IF) of anti-Ccne1 antibody

Immunofluorescence analysis of A549 cells, using Cyclin E1 Antibody. The picture on the right is treated with the synthesized peptide.
Rabbit Anti-CCNE1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS643796

Other Notes

Small volumes of anti-Ccne1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Ccne1 purchase

MBS643796 is an antibody targeting Ccne1. Also known as CCNE1, ID (G1/S-specific Cyclin-E1, CCNE). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Ccne1. It may not necessarily be applicable to this product.

NCBI GI #
171847100
NCBI GeneID
25729
NCBI Accession #
AAI62008.1
UniProt Primary Accession #
P39949
UniProt Secondary Accession #
O09138
UniProt Related Accession #
P39949
NCBI Official Full Name
Ccne1 protein
NCBI Official Synonym Full Names
cyclin E1
NCBI Official Symbol
Ccne1
NCBI Official Synonym Symbols
Ccne; CYCLE
UniProt Protein Name
G1/S-specific cyclin-E1
UniProt Gene Name
Ccne1
UniProt Synonym Gene Names
Ccne
UniProt Entry Name
CCNE1_RAT
NCBI Protein Information
G1/S-specific cyclin-E1; cyclin E

NCBI Summary for Ccne1

a cyclin-like protein; may be involved in growth stimulation [RGD, Feb 2006]

UniProt Comments for Ccne1

Function: Essential for the control of the cell cycle at the G1/S (start) transition.

Subunit structure: Interacts with a member of the CDK2/CDK protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Found in a complex with CDK2, CABLES1 and CCNA1. Part of a complex consisting of UHRF2, CDK2 and CCNE1. Interacts directly with UHRF2; the interaction ubiquitinates CCNE1 and appears to occur independently of CCNE1 phosphorylation

By similarity.

Subcellular location: Nucleus

By similarity.

Post-translational modification: Phosphorylation of both Thr-396 by GSK3 and Ser-400 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7

By similarity.Ubiquitinated by UHRF2; appears to occur independently of phosphorylation

By similarity.

Sequence similarities: Belongs to the cyclin family. Cyclin E subfamily.

Sequence caution: The sequence BAA03116.1 differs from that shown. Reason: Frameshift at position 15.
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Research Articles on Ccne1

1. Data indicate that proline-mediated mTOR activation and collagen synthesis were found critical for hepatocyte growth factor (HGF)-induced DNA synthesis, partly via the sufficient accumulation of cyclin-E1.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Ccne1 antibody

 Products by Pathway  Pathway Diagram
 ATM Pathway antibodies  ATM Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Checkpoints Pathway antibodies  Cell Cycle Checkpoints Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cyclin A:Cdk2-associated Events At S Phase Entry Pathway antibodies  Cyclin A:Cdk2-associated Events At S Phase Entry Pathway Diagram

Organs/Tissues associated with anti-Ccne1 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >23 publications with Ccne1 and Liver
 Lung Antibodies  >13 publications with Ccne1 and Lung
 Brain Antibodies  >10 publications with Ccne1 and Brain
 Kidney Antibodies  >9 publications with Ccne1 and Kidney
 Embryonic Tissue Antibodies  >4 publications with Ccne1 and Embryonic Tissue
 Placenta Antibodies  >3 publications with Ccne1 and Placenta
 Pancreas Antibodies  >2 publications with Ccne1 and Pancreas
 Heart Antibodies  >2 publications with Ccne1 and Heart
 Connective Tissue Antibodies  >1 publications with Ccne1 and Connective Tissue
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