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SLC12A4 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS643908

MBS643908 is an antibody targeting SLC12A4. Also known as SLC12A4 (Solute Carrier Family 12 Member 4, Electroneutral Potassium-chloride Cotransporter 1, Erythroid K-Cl Cotransporter 1, hKCC1, KCC1). Reactive in Human. Host: Goat. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SLC12A4, Polyclonal Antibody
Full Product Name
SLC12A4 (Solute Carrier Family 12 Member 4, Electroneutral Potassium-chloride Cotransporter 1, Erythroid K-Cl Cotransporter 1, hKCC1, KCC1)
Product Gene Name
anti-SLC12A4 antibody
Product Synonym Names
Anti -SLC12A4 (Solute Carrier Family 12 Member 4, Electroneutral Potassium-chloride Cotransporter 1, Erythroid K-Cl Cotransporter 1, hKCC1, KCC1)
Clonality
Polyclonal
Host
Goat
Species Reactivity
Human
Form/Format
Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes human SLC12A4. Species sequence homology: Canine, mouse and rat.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
120,650 Da
Chromosome Location
Chromosome: 16; NC_000016.9 (67977377..68002597, complement). Location: 16q22.1
OMIM
604119
3D Structure
ModBase 3D Structure for Q9UP95
Immunogen
Synthetic peptide corresponding to C-DPSHAPDNFRE from an internal region of human SLC12A4.

Product Categories/Family for anti-SLC12A4 antibody

Antibodies; Abs to Ion Channel
Applications Tested/Suitable for anti-SLC12A4 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-SLC12A4 antibody
Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: ELISA: 1:4000
Western Blot: 0.3-1ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml

Immunohistochemistry (IHC) of anti-SLC12A4 antibody

Human Placenta (formalin-fixed, paraffin-embedded) stained with SLC12A4 antibody at 5ug/ml followed by biotinylated goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.
Goat Anti-SLC12A4 Polyclonal Antibody – Cat# MBS643908

Chemiluminescence of anti-SLC12A4 antibody

Antibody (0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-SLC12A4 Polyclonal Antibody, Chemiluminescence – Cat# MBS643908

Other Notes

Small volumes of anti-SLC12A4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SLC12A4. It may not necessarily be applicable to this product.

NCBI GI #
225579067
NCBI GeneID
6560
NCBI Accession #
NP_001139436.1
UniProt Primary Accession #
Q9UP95
UniProt Secondary Accession #
O60632; O75893; Q13953; Q96LD5; B4DF69; B4DR04; B4DZ82; B7ZAV0; F5H066; F5H0S9; F5H3C0
UniProt Related Accession #
Q9UP95
GenBank Nucleotide #
NM_001145964.1
NCBI Official Full Name
solute carrier family 12 member 4 isoform e
NCBI Official Synonym Full Names
solute carrier family 12 (potassium/chloride transporter), member 4
NCBI Official Symbol
SLC12A4
NCBI Official Synonym Symbols
KCC1
UniProt Protein Name
Solute carrier family 12 member 4
UniProt Synonym Protein Names
Electroneutral potassium-chloride cotransporter 1; Erythroid K-Cl cotransporter 1
Protein Family
Solute carrier family
UniProt Gene Name
SLC12A4
UniProt Synonym Gene Names
KCC1; hKCC1
UniProt Entry Name
S12A4_HUMAN
NCBI Protein Information
solute carrier family 12 member 4; hKCC1; erythroid K-Cl cotransporter 1; potassium/chloride cotransporter 1; electroneutral potassium-chloride cotransporter 1; solute carrier family 12 (potassium/chloride transporters), member 4

NCBI Summary for SLC12A4

This gene encodes a member of the SLC12A transporter family. The encoded protein mediates the coupled movement of potassium and chloride ions across the plasma membrane. This gene is expressed ubiquitously. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jan 2013]
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UniProt Comments for SLC12A4

KCC1: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells. May be involved in the regulation of basolateral Cl(-) exit in NaCl absorbing epithelia. Isoform 4 has no transport activity. Belongs to the SLC12A transporter family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, SLC family; Membrane protein, integral; Membrane protein, multi-pass; Transporter

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: membrane; lysosomal membrane; integral to plasma membrane; plasma membrane

Molecular Function: protein kinase binding; potassium:chloride symporter activity

Biological Process: transport; chloride transport; ion transport; cell volume homeostasis; transmembrane transport; potassium ion transport
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Research Articles on SLC12A4

1. The Wnk3 protein isoforms have a similar effect on SLC12 cotransporters. NKCC1/2 and NCC were inhibited, even in hypertonicity, while KCCs were activated, even in isotonic conditions.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-SLC12A4 antibody

 Products by Pathway  Pathway Diagram
 Cation-coupled Chloride Cotransporters Pathway antibodies  Cation-coupled Chloride Cotransporters Pathway Diagram
 SLC-mediated Transmembrane Transport Pathway antibodies  SLC-mediated Transmembrane Transport Pathway Diagram
 Transmembrane Transport Of Small Molecules Pathway antibodies  Transmembrane Transport Of Small Molecules Pathway Diagram
 Transport Of Inorganic Cations/anions And Amino Acids/oligopeptides Pathway antibodies  Transport Of Inorganic Cations/anions And Amino Acids/oligopeptides Pathway Diagram

Diseases associated with anti-SLC12A4 antibody

 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >2 publications with SLC12A4 and Liver Diseases
 Carcinoma Antibodies  >1 publications with SLC12A4 and Carcinoma
 Liver Neoplasms Antibodies  >1 publications with SLC12A4 and Liver Neoplasms
 Necrosis Antibodies  >1 publications with SLC12A4 and Necrosis
 Neoplasms Antibodies  >1 publications with SLC12A4 and Neoplasms
 Carcinoma, Hepatocellular Antibodies  >1 publications with SLC12A4 and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-SLC12A4 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >2 publications with SLC12A4 and Muscle
 Blood Antibodies  >2 publications with SLC12A4 and Blood
 Kidney Antibodies  >2 publications with SLC12A4 and Kidney
 Testis Antibodies  >1 publications with SLC12A4 and Testis
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