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RBFOX2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS643912

This gene is one of several human genes similar to the C. elegans gene Fox-1. This gene encodes an RNA binding protein that is thought to be a key regulator of alternative exon splicing in the nervous system and other cell types. The protein binds to a… More details

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Product Overview

Online Datasheet in PDF
Product Name
RBM9 (RBFOX2), Monoclonal Antibody
Full Product Name
RBM9 (RNA Binding Motif Protein 9, RNA Binding Protein 9, Hexaribonucleotide Binding Protein 2, HRNBP2, Repressor of Tamoxifen Transcriptional Activity, RTA)
Product Gene Name
anti-RBFOX2 antibody
Product Synonym Names
Anti -RBM9 (RNA Binding Motif Protein 9, RNA Binding Protein 9, Hexaribonucleotide Binding Protein 2, HRNBP2, Repressor of Tamoxifen Transcriptional Activity, RTA)
Immunogen Sequence
MEKKKMVTQG NQEPTTTPDA MVQPFTTIPF PPPPQNGIPT EYGVPHTQDY AGQTGEHNLT LYGSTQAHGE QSSNSPSTQN GSLTTEGGAQ TDGQQSQTQS
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
4G3
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human RBM9. Species Crossreactivity: mouse.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
41,374 Da
OMIM
612149
3D Structure
ModBase 3D Structure for O43251
Immunogen
Partial recombinant corresponding to aa1-100 from RBM9 (AAH13115) with GST tag. MW of the GST tag alone is 26kD.

Product Categories/Family for anti-RBFOX2 antibody

Antibodies; Abs to RNA Binding Proteins
Applications Tested/Suitable for anti-RBFOX2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-RBFOX2 antibody
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry.
Dilution: Immunofluorescence: 10ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml

Western Blot (WB) of anti-RBFOX2 antibody

Western Blot analysis of RBM9 expression in transfected 293T cell line by RBM9 monoclonal antibody|Lane 1: RBM9 transfected lysate (40.4kD).|Lane 2: Non-transfected lysate.
Mouse Anti-RBFOX2 Monoclonal Antibody – Cat# MBS643912

Immunohistochemistry (IHC) of anti-RBFOX2 antibody

Immunoperoxidase of monoclonal antibody to RBM9 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml]
Mouse Anti-RBFOX2 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS643912

Immunofluorescence (IF) of anti-RBFOX2 antibody

Immunofluorescence of monoclonal antibody to RBM9 on HeLa cell. [antibody concentration 10ug/ml]
Mouse Anti-RBFOX2 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS643912

Testing Data of anti-RBFOX2 antibody

Detection limit for recombinant GST tagged RBM9 is ~0.1ng/ml as a capture antibody.
Mouse Anti-RBFOX2 Monoclonal Antibody, Testing Data – Cat# MBS643912

Western Blot (WB) of anti-RBFOX2 antibody

Western blot analysis of RBM9 over-expressed 293 cell line, cotransfected with RBM9 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RBM9 monoclonal antibody.|GAPDH (36.1kD) used as specificity and loading control.
Mouse Anti-RBFOX2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS643912

Western Blot (WB) of anti-RBFOX2 antibody

RBM9 monoclonal antibody Western Blot analysis of RBM9 expression in NIH/3T3.
Mouse Anti-RBFOX2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS643912

Western Blot (WB) of anti-RBFOX2 antibody

Western Blot detection against Immunogen (37kD) .
Mouse Anti-RBFOX2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS643912

Other Notes

Small volumes of anti-RBFOX2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RBFOX2 purchase

MBS643912 is an antibody targeting RBFOX2. Also known as RBM9 (RNA Binding Motif Protein 9, RNA Binding Protein 9, Hexaribonucleotide Binding Protein 2, HRNBP2, Repressor of Tamoxifen Transcriptional Activity, RTA). Reactive in Human, Mouse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for RBFOX2. It may not necessarily be applicable to this product.

NCBI GI #
47678649
NCBI Accession #
CAG30445.1
UniProt Primary Accession #
O43251
UniProt Secondary Accession #
Q0PRL5; Q0VH35; Q5TF71; Q6IC09; Q8TD00; Q8WYB1; Q96DZ6; A4F5G8; A8K5Z5; B0QYY8; B0QYY9
NCBI Official Full Name
RBM9
UniProt Protein Name
RNA binding protein fox-1 homolog 2
UniProt Synonym Protein Names
Fox-1 homolog B; Hexaribonucleotide-binding protein 2; RNA-binding motif protein 9; RNA-binding protein 9; Repressor of tamoxifen transcriptional activity
Protein Family
RNA binding protein
UniProt Gene Name
RBFOX2
UniProt Synonym Gene Names
FOX2; HRNBP2; RBM9; RTA
UniProt Entry Name
RFOX2_HUMAN

UniProt Comments for RBFOX2

RBM9: RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. Prevents binding of U2AF2 to the 3'-splice site. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis. RNA-binding protein that seems to act as a coregulatory factor of ER-alpha. 10 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Nuclear receptor co-regulator; Transcription, coactivator/corepressor; RNA splicing

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: mRNA binding; protein binding; RNA binding; nucleotide binding; transcription factor binding; transcription corepressor activity

Biological Process: estrogen receptor signaling pathway; RNA metabolic process; RNA splicing; dendrite morphogenesis; radial glia guided migration of Purkinje cell; neuromuscular process controlling balance; mRNA processing; negative regulation of transcription, DNA-dependent; regulation of alternative nuclear mRNA splicing, via spliceosome; regulation of cell proliferation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-RBFOX2 antibody

 Disease Name  Pubmed Publications
 Neurobehavioral Manifestations Antibodies  >1 publications with RBFOX2 and Neurobehavioral Manifestations
 Congenital Abnormalities Antibodies  >1 publications with RBFOX2 and Congenital Abnormalities
 Breast Neoplasms Antibodies  >1 publications with RBFOX2 and Breast Neoplasms
 Neoplasms Antibodies  >1 publications with RBFOX2 and Neoplasms
 Nervous System Diseases Antibodies  >1 publications with RBFOX2 and Nervous System Diseases
 Brain Diseases Antibodies  >1 publications with RBFOX2 and Brain Diseases

Organs/Tissues associated with anti-RBFOX2 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >8 publications with RBFOX2 and Brain
 Muscle Antibodies  >7 publications with RBFOX2 and Muscle
 Nerve Antibodies  >5 publications with RBFOX2 and Nerve
 Heart Antibodies  >5 publications with RBFOX2 and Heart
 Embryonic Tissue Antibodies  >3 publications with RBFOX2 and Embryonic Tissue
 Connective Tissue Antibodies  >1 publications with RBFOX2 and Connective Tissue
 Skin Antibodies  >1 publications with RBFOX2 and Skin
 Eye Antibodies  >1 publications with RBFOX2 and Eye
 Lung Antibodies  >1 publications with RBFOX2 and Lung
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