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CD63 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS643973

CD63 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CD63, Polyclonal Antibody
Full Product Name
CD63, CT (CD63, MLA1, TSPAN30, CD63 antigen, Granulophysin, Lysosomal-associated membrane protein 3, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, CD63)
Product Gene Name
anti-CD63 antibody
Product Synonym Names
Anti -CD63, CT (CD63, MLA1, TSPAN30, CD63 antigen, Granulophysin, Lysosomal-associated membrane protein 3, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, CD63)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
25,637 Da
Chromosome Location
Chromosome: 12; NC_000012.11 (56119227..56123457, complement). Location: 12q12-q13
OMIM
155740
3D Structure
ModBase 3D Structure for P08962
Immunogen
CD63 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-200 amino acids from the C-terminal region of human CD63. CD63 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-200 amino acids from the C-terminal region of human CD63.

Product Categories/Family for anti-CD63 antibody

Antibodies; Abs to CD Markers
Applications Tested/Suitable for anti-CD63 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-CD63 antibody
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-CD63 antibody

Western Blot analysis in MDA-MB435 cell line lysates (35ug/lane) using MBS643973. This demonstrates that MBS643973 detected the CD63 protein (arrow).
Rabbit Anti-CD63 Polyclonal Antibody – Cat# MBS643973

Western Blot (WB) of anti-CD63 antibody

Western Blot using MBS643973 (1:2000) Lane 1: A2058 whole cell lysates Lane 2: A375 whole cell lysates Lane 3: HL-60 whole cell lysates Lane 4: THP-1 whole cell lysates Lane 5: U-87 MG whole cell lysates Lysates/proteins at 20ug/lane. Secondary IgG (HRP) Goat Anti-Rabbit at 10,000. Predicted band size: 25kD. Blocking/Dilution buffer: 5% NFDM/TBST.
Rabbit Anti-CD63 Polyclonal Antibody, Western Blot (WB) – Cat# MBS643973

Immunohistochemistry (IHC) of anti-CD63 antibody

Immunohistochemistry analysis of CD63 in formalin fixed paraffin embedded.human melanoma tissue sections using MBS643973. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5hr at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with MBS643973 (1:25) for 1hr at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Rabbit Anti-CD63 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS643973

Immunohistochemistry (IHC) of anti-CD63 antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma using MBS643973 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS643973 for immunohistochemistry.
Rabbit Anti-CD63 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS643973

Other Notes

Small volumes of anti-CD63 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD63 purchase

MBS643973 is an antibody targeting CD63. Also known as CD63, CT (CD63, MLA1, TSPAN30, CD63 antigen, Granulophysin, Lysosomal-associated membrane protein 3, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, CD63). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CD63. It may not necessarily be applicable to this product.

NCBI GI #
383872547
NCBI GeneID
967
NCBI Accession #
NP_001244330.1
UniProt Primary Accession #
P08962
UniProt Secondary Accession #
Q5TZP3; Q8N6Z9; Q9UCG6; F8VZE2
UniProt Related Accession #
P08962
GenBank Nucleotide #
NM_001257401.1
NCBI Official Full Name
CD63 antigen isoform D
NCBI Official Synonym Full Names
CD63 molecule
NCBI Official Symbol
CD63
NCBI Official Synonym Symbols
MLA1; ME491; LAMP-3; OMA81H; TSPAN30
UniProt Protein Name
CD63 antigen
UniProt Synonym Protein Names
Granulophysin; Lysosomal-associated membrane protein 3; LAMP-3; Melanoma-associated antigen ME491; OMA81H; Ocular melanoma-associated antigen; Tetraspanin-30
Protein Family
CD63 antigen
UniProt Gene Name
CD63
UniProt Synonym Gene Names
MLA1; TSPAN30; LAMP-3; Tspan-30
UniProt Entry Name
CD63_HUMAN
NCBI Protein Information
CD63 antigen; tspan-30; granulophysin; tetraspanin-30; melanoma-associated antigen MLA1; CD63 antigen (melanoma 1 antigen); melanoma-associated antigen ME491; ocular melanoma-associated antigen; lysosomal-associated membrane protein 3; lysosome-associated membrane glycoprotein 3

NCBI Summary for CD63

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012]
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UniProt Comments for CD63

CD63: This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation. Belongs to the tetraspanin (TM4SF) family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 12q12-q13

Cellular Component: cell surface; lysosomal membrane; late endosome membrane; integral to plasma membrane; plasma membrane; melanosome; platelet dense granule membrane; endosome membrane; intrinsic to plasma membrane

Molecular Function: protein binding

Biological Process: platelet activation; cell migration; protein transport; platelet degranulation; pigment granule maturation; cell-matrix adhesion; positive regulation of receptor internalization; blood coagulation
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Research Articles on CD63

1. Antigen-induced p38 MAPK phosphorylation in human basophils essentially contributes to CD63 upregulation

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD63 antibody

 Reference Product  PubMed Publications
 CXCR4 antibody  >12 publications with CD63 and CXCR4
 LAMP2 antibody  >9 publications with CD63 and LAMP2
 TIMP1 antibody  >5 publications with CD63 and TIMP1
 UBC antibody  >3 publications with CD63 and UBC
 NPY antibody  >2 publications with CD63 and NPY
 SELP antibody  >2 publications with CD63 and SELP
 SYT7 antibody  >1 publications with CD63 and SYT7
 ITGB1 antibody  >1 publications with CD63 and ITGB1

Pathways associated with anti-CD63 antibody

 Products by Pathway  Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
 Platelet Degranulation Pathway antibodies  Platelet Degranulation Pathway Diagram
 Proteoglycans In Cancer Pathway antibodies  Proteoglycans In Cancer Pathway Diagram
 Proteoglycans In Cancer Pathway antibodies  Proteoglycans In Cancer Pathway Diagram
 Response To Elevated Platelet Cytosolic Ca2+ Pathway antibodies  Response To Elevated Platelet Cytosolic Ca2+ Pathway Diagram

Diseases associated with anti-CD63 antibody

 Disease Name  Pubmed Publications
 Immune System Diseases Antibodies  >279 publications with CD63 and Immune System Diseases
 Neoplasms Antibodies  >172 publications with CD63 and Neoplasms
 Cardiovascular Diseases Antibodies  >151 publications with CD63 and Cardiovascular Diseases
 Inflammation Antibodies  >108 publications with CD63 and Inflammation
 Heart Diseases Antibodies  >51 publications with CD63 and Heart Diseases
 Nervous System Diseases Antibodies  >50 publications with CD63 and Nervous System Diseases
 Carcinoma Antibodies  >44 publications with CD63 and Carcinoma
 Necrosis Antibodies  >37 publications with CD63 and Necrosis
 Kidney Diseases Antibodies  >24 publications with CD63 and Kidney Diseases
 Neoplasm Metastasis Antibodies  >23 publications with CD63 and Neoplasm Metastasis

Organs/Tissues associated with anti-CD63 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >905 publications with CD63 and Blood
 Skin Antibodies  >153 publications with CD63 and Skin
 Vascular Antibodies  >120 publications with CD63 and Vascular
 Bone Antibodies  >59 publications with CD63 and Bone
 Lung Antibodies  >58 publications with CD63 and Lung
 Bone Marrow Antibodies  >46 publications with CD63 and Bone Marrow
 Kidney Antibodies  >45 publications with CD63 and Kidney
 Heart Antibodies  >38 publications with CD63 and Heart
 Brain Antibodies  >36 publications with CD63 and Brain
 Muscle Antibodies  >35 publications with CD63 and Muscle
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