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FOLR2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS644139

FOLR2 is a member of the folate receptor (FOLR) family, and these genes exist in a cluster on chromosome 11. Members of this gene family have a high affinity for folic acid and for several reduced folic acid derivatives, and they mediate delivery of… More details

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Product Overview

Online Datasheet in PDF
Product Name
FOLR2, Polyclonal Antibody
Full Product Name
FOLR2, NT (FOLR2, Folate receptor beta, Folate receptor 2, Folate receptor, fetal/placental, Placental folate-binding protein)
Product Gene Name
Product Synonym Names
Anti -FOLR2, NT (FOLR2, Folate receptor beta, Folate receptor 2, Folate receptor, fetal/placental, Placental folate-binding protein)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human, mouse
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
29,280 Da
Chromosome Location
Chromosome: 11; NC_000011.9 (71927819..71932994). Location: 11q13.3-q13.5
OMIM
136425
3D Structure
ModBase 3D Structure for P14207
Immunogen
FOLR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-55 amino acids from the N-terminal region of human FOLR2.
Applications Tested/Suitable for anti-FOLR2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-FOLR2 antibody
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-FOLR2 antibody

Western Blot analysis in mouse heart tissue lysates (35ug/lane) using MBS644139. FOLR2 (arrow) was detected using MBS644139.
Rabbit Anti-FOLR2 Polyclonal Antibody – Cat# MBS644139

Western Blot (WB) of anti-FOLR2 antibody

Western Blot analysis of lysate from human placenta tissue (20ug) using MBS644139 (1:1000). A goat anti-rabbit IgG H&L(HRP) was used as the secondary antibody.
Rabbit Anti-FOLR2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS644139

Western Blot (WB) of anti-FOLR2 antibody

Western Blot analysis of lysates from MDA-MB-453 cell line and human placenta tissue lysate (20ug/lane, from left to right) using MBS644139 (1:1000/lane). A goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody.
Rabbit Anti-FOLR2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS644139

Immunohistochemistry (IHC) of anti-FOLR2 antibody

Immunohistochemical staining of formalin fixed, paraffin-embedded human spleen using MBS644139. HRP-conjugated secondary antibody and DAB staining were used for visualizaton of antigen.
Rabbit Anti-FOLR2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS644139

Flow Cytometry (FC/FACS) of anti-FOLR2 antibody

Flow Cytometric analysis of HL-60 cells using MBS644139 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibody was used for the analysis.
Rabbit Anti-FOLR2 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS644139

Other Notes

Small volumes of anti-FOLR2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FOLR2 purchase

MBS644139 is an antibody targeting FOLR2. Also known as FOLR2, NT (FOLR2, Folate receptor beta, Folate receptor 2, Folate receptor, fetal/placental, Placental folate-binding protein). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for FOLR2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
folate receptor beta
NCBI Official Synonym Full Names
folate receptor 2 (fetal)
NCBI Official Symbol
FOLR2
NCBI Official Synonym Symbols
FBP; FR-P3; FR-BETA; BETA-HFR; FBP/PL-1
UniProt Protein Name
Folate receptor beta
UniProt Synonym Protein Names
Folate receptor 2; Folate receptor, fetal/placental; Placental folate-binding protein
Protein Family
UniProt Gene Name
FOLR2
UniProt Synonym Gene Names
FR-beta; FBP
UniProt Entry Name
FOLR2_HUMAN
NCBI Protein Information
folate receptor beta; folate receptor, beta; folate receptor, fetal/placental; placental folate-binding protein; folate-binding protein, fetal/placental

NCBI Summary for FOLR2

The protein encoded by this gene is a member of the folate receptor (FOLR) family, and these genes exist in a cluster on chromosome 11. Members of this gene family have a high affinity for folic acid and for several reduced folic acid derivatives, and they mediate delivery of 5-methyltetrahydrofolate to the interior of cells. This protein has a 68% and 79% sequence homology with the FOLR1 and FOLR3 proteins, respectively. Although this protein was originally thought to be specific to placenta, it can also exist in other tissues, and it may play a role in the transport of methotrexate in synovial macrophages in rheumatoid arthritis patients. Multiple transcript variants that encode the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for FOLR2

FOLR2: Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. Belongs to the folate receptor family.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 11q13.3-q13.5

Cellular Component: anchored to external side of plasma membrane; membrane; extracellular region

Molecular Function: folic acid transporter activity; folic acid binding

Biological Process: folic acid transport
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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