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MGEA5 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS644264

Cleaves GlcNAc but not GalNAc from glycopeptides. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc. Possesses hyaluronidase activity. Acetylates 'Lys-8' of histone H4 and 'Lys-14' of histone H3. More details

$880 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MGEA5, Monoclonal Antibody
Full Product Name
MGEA5 (Bifunctional Protein NCOAT, Meningioma-expressed Antigen 5, Nuclear Cytoplasmic O-GlcNAcase and Acetyltransferase, HEXC, KIAA0679, MEA5, FLJ11229, FLJ23355)
Product Gene Name
anti-MGEA5 antibody
Product Synonym Names
Anti -MGEA5 (Bifunctional Protein NCOAT, Meningioma-expressed Antigen 5, Nuclear Cytoplasmic O-GlcNAcase and Acetyltransferase, HEXC, KIAA0679, MEA5, FLJ11229, FLJ23355)
Immunogen Sequence
SFHEEQEVLP ETFLANFPSL IKMDIHKKVT DPSVAKSMMA CLLSSLKANG SRGAFCEVRP DDKRILEFYS KLGCFEIAKM EGFPKDVVIL GRSL
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
1C7
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human MGEA5.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
102,915 Da
Chromosome Location
Chromosome: 10; NC_000010.10 (103544200..103578930, complement). Location: 10q24.1-q24.3
OMIM
604039
3D Structure
ModBase 3D Structure for O60502
Immunogen
Partial recombinant corresponding to aa823-916 from human MGEA5 (NP_036347) with GST tag. MW of the GST tag alone is 26kD.

Product Categories/Family for anti-MGEA5 antibody

Antibodies; Abs to Enzymes; Glycosidase
Applications Tested/Suitable for anti-MGEA5 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-MGEA5 antibody
Suitable for use in ELISA and Western Blot.

Western Blot (WB) of anti-MGEA5 antibody

Western Blot detection against Immunogen (36.45kD).
Mouse Anti-MGEA5 Monoclonal Antibody – Cat# MBS644264

Testing Data of anti-MGEA5 antibody

Detection limit for recombinant GST tagged MGEA5 is 0.3ng/ml as a capture antibody.
Mouse Anti-MGEA5 Monoclonal Antibody, Testing Data – Cat# MBS644264

Other Notes

Small volumes of anti-MGEA5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MGEA5 purchase

MBS644264 is an antibody targeting MGEA5. Also known as MGEA5 (Bifunctional Protein NCOAT, Meningioma-expressed Antigen 5, Nuclear Cytoplasmic O-GlcNAcase and Acetyltransferase, HEXC, KIAA0679, MEA5, FLJ11229, FLJ23355). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for MGEA5. It may not necessarily be applicable to this product.

NCBI GI #
34192983
NCBI GeneID
10724
NCBI Accession #
AAH39583.2
UniProt Primary Accession #
O60502
UniProt Secondary Accession #
O75166; Q86WV0; Q8IV98; Q9BVA5; Q9HAR0; B7WPB9; D3DR79
UniProt Related Accession #
O60502
NCBI Official Full Name
MGEA5 protein
NCBI Official Synonym Full Names
meningioma expressed antigen 5 (hyaluronidase)
NCBI Official Symbol
MGEA5
NCBI Official Synonym Symbols
OGA; MEA5; NCOAT
UniProt Protein Name
Protein O-GlcNAcase
UniProt Synonym Protein Names
Beta-N-acetylglucosaminidase; Beta-N-acetylhexosaminidaseBeta-hexosaminidaseMeningioma-expressed antigen 5
Protein Family
Protein O-GlcNAcase
UniProt Gene Name
MGEA5
UniProt Synonym Gene Names
OGA
UniProt Entry Name
NCOAT_HUMAN
NCBI Protein Information
bifunctional protein NCOAT; O-GlcNAcase; hyaluronidase in meningioma; meningioma-expressed antigen 5; nuclear cytoplasmic O-GlcNAcase and acetyltransferase

NCBI Summary for MGEA5

The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM, Mar 2008]
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UniProt Comments for MGEA5

OGA: Cleaves GlcNAc but not GalNAc from glycopeptides. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl- beta-GalNAc or p-nitrophenyl-alpha-GlcNAc. Possesses hyaluronidase activity. Acetylates 'Lys-8' of histone H4 and 'Lys-14' of histone H3. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.2.1.169; EC 3.2.1.52; Acetyltransferase; Hydrolase

Chromosomal Location of Human Ortholog: 10q24.1-q24.3

Cellular Component: membrane; nucleus; cytosol

Molecular Function: beta-N-acetylglucosaminidase activity; histone acetyltransferase activity; hyalurononglucosaminidase activity

Biological Process: positive regulation of DNA metabolic process; positive regulation of protein complex disassembly; dATP metabolic process; positive regulation of proteolysis; protein deglycosylation; N-acetylglucosamine metabolic process; positive regulation of insulin secretion; positive regulation of growth hormone secretion; positive regulation of glucose import; negative regulation of protein amino acid glycosylation; positive regulation of mitochondrial depolarization; positive regulation of cell killing; response to steroid hormone stimulus; histone acetylation; glycoprotein catabolic process; protein targeting to membrane; aging
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Research Articles on MGEA5

1. O-linked beta-N-acetylglucosaminylation (O-GlcNAcylation) in primary and metastatic colorectal cancer clones and effect of N-acetyl-beta-D-glucosaminidase silencing on cell phenotype and transcriptome.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MGEA5 antibody

 Reference Product  PubMed Publications
 OGT antibody  >5 publications with MGEA5 and OGT

Diseases associated with anti-MGEA5 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >3 publications with MGEA5 and Inflammation
 Kidney Diseases Antibodies  >3 publications with MGEA5 and Kidney Diseases
 Endometrial Neoplasms Antibodies  >1 publications with MGEA5 and Endometrial Neoplasms
 Cardiovascular Diseases Antibodies  >1 publications with MGEA5 and Cardiovascular Diseases
 Necrosis Antibodies  >1 publications with MGEA5 and Necrosis

Organs/Tissues associated with anti-MGEA5 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >5 publications with MGEA5 and Blood
 Bladder Antibodies  >3 publications with MGEA5 and Bladder
 Kidney Antibodies  >3 publications with MGEA5 and Kidney
 Lymph Antibodies  >2 publications with MGEA5 and Lymph
 Vascular Antibodies  >2 publications with MGEA5 and Vascular
 Muscle Antibodies  >2 publications with MGEA5 and Muscle
 Lymph Node Antibodies  >2 publications with MGEA5 and Lymph Node
 Skin Antibodies  >2 publications with MGEA5 and Skin
 Embryonic Tissue Antibodies  >1 publications with MGEA5 and Embryonic Tissue
 Brain Antibodies  >1 publications with MGEA5 and Brain
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