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ERGIC3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS644283

MBS644283 is an antibody targeting ERGIC3. Also known as ERGIC3, NT (ERGIC3, C20orf47, ERV46, SDBCAG84, Endoplasmic reticulum-Golgi intermediate compartment protein 3, Serologically defined breast cancer antigen NY-BR-84). Reactive in Human. Host: Rabbit.… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ERGIC3, Polyclonal Antibody
Full Product Name
ERGIC3, NT (ERGIC3, C20orf47, ERV46, SDBCAG84, Endoplasmic reticulum-Golgi intermediate compartment protein 3, Serologically defined breast cancer antigen NY-BR-84)
Product Gene Name
Product Synonym Names
Anti -ERGIC3, NT (ERGIC3, C20orf47, ERV46, SDBCAG84, Endoplasmic reticulum-Golgi intermediate compartment protein 3, Serologically defined breast cancer antigen NY-BR-84)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
43,222 Da
Chromosome Location
Chromosome: 20; NC_000020.10 (34129778..34145405). Location: 20pter-q12
3D Structure
ModBase 3D Structure for Q9Y282
Immunogen
ERGIC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human ERGIC3.
Applications Tested/Suitable for anti-ERGIC3 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-ERGIC3 antibody
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100

Western Blot (WB) of anti-ERGIC3 antibody

Western blot analysis of ERGIC3 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). ERGIC3 (arrow) was detected using the purified Pab.
Rabbit Anti-ERGIC3 Polyclonal Antibody – Cat# MBS644283

Immunohistochemistry (IHC) of anti-ERGIC3 antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ERGIC3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-ERGIC3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS644283

Western Blot (WB) of anti-ERGIC3 antibody

Western blot analysis of ERGIC3 (arrow) using rabbit polyclonal ERGIC3 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ERGIC3 gene.
Rabbit Anti-ERGIC3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS644283

Other Notes

Small volumes of anti-ERGIC3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ERGIC3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
endoplasmic reticulum-Golgi intermediate compartment protein 3 isoform a
NCBI Official Synonym Full Names
ERGIC and golgi 3
NCBI Official Symbol
ERGIC3
NCBI Official Synonym Symbols
Erv46; CGI-54; PRO0989; C20orf47; NY-BR-84; SDBCAG84; dJ477O4.2
UniProt Protein Name
Endoplasmic reticulum-Golgi intermediate compartment protein 3
UniProt Synonym Protein Names
Serologically defined breast cancer antigen NY-BR-84
UniProt Gene Name
ERGIC3
UniProt Synonym Gene Names
C20orf47; ERV46; SDBCAG84
UniProt Entry Name
ERGI3_HUMAN
NCBI Protein Information
endoplasmic reticulum-Golgi intermediate compartment protein 3; endoplasmic reticulum-localized protein ERp43; serologically defined breast cancer antigen 84; serologically defined breast cancer antigen NY-BR-84

UniProt Comments for ERGIC3

SDBCAG84: Possible role in transport between endoplasmic reticulum and Golgi. Belongs to the ERGIC family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Motility/polarity/chemotaxis; Vesicle

Chromosomal Location of Human Ortholog: 20pter-q12

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; membrane; integral to membrane

Biological Process: vesicle-mediated transport
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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