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CD1E Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS644289

CD1E encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of… More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CD1E, Polyclonal Antibody
Full Product Name
CD1E, ID (CD1E, T-cell surface glycoprotein CD1e, membrane-associated, R2G1, CD1e, T-cell surface glycoprotein CD1e, soluble)
Product Synonym Names
Anti -CD1E, ID (CD1E, T-cell surface glycoprotein CD1e, membrane-associated, R2G1, CD1e, T-cell surface glycoprotein CD1e, soluble)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
CD1E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 274-304 amino acids from the Central region of human CD1E.

Product Categories/Family for anti-CD1E antibody

Antibodies; Abs to CD Markers
Applications Tested/Suitable for anti-CD1E antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-CD1E antibody
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Immunofluorescence: 1:10-50

Western Blot (WB) of anti-CD1E antibody

CD1E Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the CD1E antibody detected the CD1E protein (arrow).
Rabbit Anti-CD1E Polyclonal Antibody – Cat# MBS644289

Immunohistochemistry (IHC) of anti-CD1E antibody

CD1E Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CD1E Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-CD1E Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS644289

Flow Cytometry (FC/FACS) of anti-CD1E antibody

CD1E Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-CD1E Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS644289

Immunofluorescence (IF) of anti-CD1E antibody

Confocal immunofluorescent analysis of CD1E Antibody (Center) with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-CD1E Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS644289

Other Notes

Small volumes of anti-CD1E antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD1E purchase

MBS644289 is an antibody targeting CD1E. Also known as CD1E, ID (CD1E, T-cell surface glycoprotein CD1e, membrane-associated, R2G1, CD1e, T-cell surface glycoprotein CD1e, soluble). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CD1E. It may not necessarily be applicable to this product.

NCBI GI #
14030479
NCBI Accession #
AAK52914.1
NCBI Official Full Name
CD1E
Protein Family
T-cell surface glycoprotein

UniProt Comments for CD1E

T-cell surface glycoprotein CD1e, soluble binds diacetylated lipids, including phosphatidyl inositides and diacylated sulfoglycolipids, and is required for the presentation of glycolipid antigens on the cell surface. The membrane-associated form is not active.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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