Rabbit Anti-PDHA2 Polyclonal Antibody – Cat# MBS644471

Western Blot analysis of PDHA2 expression in transfected 293T cell line by PDHA2 polyclonal antibody. Lane 1: PDHA2 transfected lysate (42.9kD). Lane 2: Non-transfected lysate.

PDHA2 Antibody – Rabbit Polyclonal

Name: PDHA2 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS644471
Price: 970 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS644471

MBS644471 is an antibody targeting PDHA2. Also known as PDHA2 (PDHAL, Pyruvate Dehydrogenase E1 Component Subunit alpha, Testis-specific Form, Mitochondrial, PDHE1-A Type II, MGC149517, MGC149518). Reactive in Human. Host: Rabbit. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PDHA2, Polyclonal Antibody

Full Product Name

PDHA2 (PDHAL, Pyruvate Dehydrogenase E1 Component Subunit alpha, Testis-specific Form, Mitochondrial, PDHE1-A Type II, MGC149517, MGC149518)

Product Gene Name

anti-PDHA2 antibody

Product Synonym Names

Anti -PDHA2 (PDHAL, Pyruvate Dehydrogenase E1 Component Subunit alpha, Testis-specific Form, Mitochondrial, PDHE1-A Type II, MGC149517, MGC149518)

Immunogen Sequence

MLAAFISRVL RRVAQKSARR VLVASRNSSN DATFEIKKCD LYLLEEGPPV TTVLTRAEGL KYYRMMLTVR RMELKADQLY KQKFIRGFCH LCDGQEACCV GLEAGINPSD HVITSYRAHG VCYTRGLSVR SILAELTGRR GGCAKGKGGS MHMYTKNFYG GNGIVGAQGP LGAGIALACK YKGNDEICLT LYGDGAANQG QIAEAFNMAA LWKLPCVFIC ENNLYGMGTS TERAAASPDY YKRGNFIPGL KVDGMDVLCV REATKFAANY CRSGKGPILM ELQTYRYHGH SMSDPGVSYR TREEIQEVRS KRDPIIILQD RMVNSKLATV EELKEIGAEV RKEIDDAAQF ATTDPEPHLE ELGHHIYSSD SSFEVRGANP WIKFKSVS

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2.

Purity/Purification

Affinity Purified
Purified by Protein A affinity chromatography.

Specificity

Recognizes human PDHA2.

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

42,933 Da

Chromosome Location

Chromosome: 4; NC_000004.11 (96761239..96762625). Location: 4q22-q23

OMIM

179061

3D Structure

ModBase 3D Structure for P29803

Immunogen

Full length human PDHA2, aa1-388 (NP_005381.1).

Product Categories/Family for anti-PDHA2 antibody

Antibodies; Abs to Enzymes; Dehydrogenase

Applications Tested/Suitable for anti-PDHA2 antibody

Western Blot (WB)

Application Notes for anti-PDHA2 antibody

Suitable for use in Western Blot.

Other Notes

Small volumes of anti-PDHA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PDHA2. It may not necessarily be applicable to this product.

NCBI GI #

4885543

NCBI GeneID

5161

NCBI Accession #

NP_005381.1

UniProt Primary Accession #

P29803

UniProt Secondary Accession #

Q0VDI5; Q4VC02; Q6NXQ1; B2R9Q3

UniProt Related Accession #

P29803

GenBank Nucleotide #

NM_005390.4

NCBI Official Full Name

pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial

NCBI Official Synonym Full Names

pyruvate dehydrogenase (lipoamide) alpha 2

NCBI Official Symbol

PDHA2

NCBI Official Synonym Symbols

PDHAL

UniProt Protein Name

Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial

UniProt Synonym Protein Names

PDHE1-A type II

Protein Family

Pyruvate dehydrogenase E1 component

UniProt Gene Name

PDHA2

UniProt Synonym Gene Names

PDHAL

UniProt Entry Name

ODPAT_HUMAN

NCBI Protein Information

pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial; PDHE1-A type II; pyruvate dehydrogenase, E1-alpha polypeptide, testis specific

UniProt Comments for PDHA2

PDHA2: a testis-specific mitochondrial matrix enzyme that catalyzes the oxidative decarboxylation of pyruvate, producing acetyl-CoA and CO2. A key enzyme in controlling the balance between lipid and glucose oxidation depending on substrate availability. The pyruvate dehydrogenase (PDH) holoenzyme is a multi-enzyme complex (PDHC) that contains 20-30 copies of pyruvate decarboxylase tetramers (2 alpha:2 beta)(E1), 60 copies of dihydrolipoamide acetyltransferase (E2), six homodimers of dihydrolipoamide dehydrogenase (E3), plus E3 binding proteins. The activity of PDH is tightly regulated by phosphorylation. The phosphorylation of at least one of three specific serine residues in E1 subunit by PDHK inactivates the PDHC, while dephosphorylation by PDP restores its activity. Expressed in postmeiotic spermatogenic cells.

Protein type: Mitochondrial; EC 1.2.4.1; Carbohydrate Metabolism - butanoate; Carbohydrate Metabolism - pyruvate; Amino Acid Metabolism - valine, leucine and isoleucine biosynthesis; Oxidoreductase; Carbohydrate Metabolism - citrate (TCA) cycle; Carbohydrate Metabolism - glycolysis and gluconeogenesis

Chromosomal Location of Human Ortholog: 4q22-q23

Cellular Component: mitochondrial matrix; nucleus

Molecular Function: pyruvate dehydrogenase (acetyl-transferring) activity

Biological Process: glycolysis; tricarboxylic acid cycle; glucose metabolic process; pyruvate metabolic process

Research Articles on PDHA2

1. Expression of the PDHA1 gene was found in all somatic cells, whereas expression of PDHA2 gene was restricted to germ cells. The switch from X-linked to autosomic gene expression occurred in spermatocytes.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PDHA2 antibody

Reference Product	PubMed Publications
PDHA1 antibody	>3 publications with PDHA2 and PDHA1
LDHC antibody	>1 publications with PDHA2 and LDHC
DLD antibody	>1 publications with PDHA2 and DLD
PDHB antibody	>1 publications with PDHA2 and PDHB

Pathways associated with anti-PDHA2 antibody

Products by Pathway	Pathway Diagram
Acetylcholine Synthesis Pathway antibodies	Acetylcholine Synthesis Pathway Diagram
Butanoate Metabolism Pathway antibodies	Butanoate Metabolism Pathway Diagram
Butanoate Metabolism Pathway antibodies	Butanoate Metabolism Pathway Diagram
Carbon Metabolism Pathway antibodies	Carbon Metabolism Pathway Diagram
Carbon Metabolism Pathway antibodies	Carbon Metabolism Pathway Diagram
Citrate Cycle (TCA Cycle) Pathway antibodies	Citrate Cycle (TCA Cycle) Pathway Diagram
Citrate Cycle (TCA Cycle) Pathway antibodies	Citrate Cycle (TCA Cycle) Pathway Diagram
Conversion Of Glucose To Acetyl CoA And Entry Into The TCA Cycle Pathway antibodies	Conversion Of Glucose To Acetyl CoA And Entry Into The TCA Cycle Pathway Diagram
Glycolysis / Gluconeogenesis Pathway antibodies	Glycolysis / Gluconeogenesis Pathway Diagram
Glycolysis / Gluconeogenesis Pathway antibodies	Glycolysis / Gluconeogenesis Pathway Diagram

Organs/Tissues associated with anti-PDHA2 antibody

Organ/Tissue Name	Pubmed Publications
Testis Antibodies	>7 publications with PDHA2 and Testis