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ATGL Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS644527

Triglycerides are the most efficient form of energy storage in mammalian adipose tissue during times of caloric excess. ATGL is one of the key enzymes involved in the mobilization of fatty acids from triglyceride stores in adipose tissue, catalyzing the… More details

$835 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ATGL, Polyclonal Antibody
Full Product Name
ATGL (Patatin-like Phospholipase Domain-containing Protein 2, Adipose Triglyceride Lipase, Calcium-independent Phospholipase A2, Desnutrin, IPLA2-zeta, Pigment Epithelium-derived Factor, TTS2.2, Transport-secretion Protein 2, TTS2, PNPLA2, ATGL, FP17548)
Product Synonym Names
Anti -ATGL (Patatin-like Phospholipase Domain-containing Protein 2, Adipose Triglyceride Lipase, Calcium-independent Phospholipase A2, Desnutrin, IPLA2-zeta, Pigment Epithelium-derived Factor, TTS2.2, Transport-secretion Protein 2, TTS2, PNPLA2, ATGL, FP17548)
Clonality
Polyclonal
Host
Goat
Species Reactivity
Bovine, Canine, Human, Mouse, Porcine, Rat
Form/Format
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes human PNPLA2. Species sequence homology: Bovine, canine, mouse, porcine and rat.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-NIIEVSKEARKR, from human PNPLA2, at the internal region of the protein (NP_065109.1).
Applications Tested/Suitable for anti-ATGL antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-ATGL antibody
Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: ELISA: 1:32,000
Western Blot: 0.5-2ug/ml, observed in human adipose, heart and testis lysates on ~60kD bands
Immunohistochemistry (Paraffin): 2-3ug/ml

Chemiluminescence of anti-ATGL antibody

Staining of Human Adipose lysate (35ug protein in RIPA buffer) using MBS644527 (0.5ug/ml). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-ATGL Polyclonal Antibody – Cat# MBS644527

Immunohistochemistry (IHC) of anti-ATGL antibody

Staining of paraffin embedded Human Heart using MBS644527 (2.5ug/ml). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Goat Anti-ATGL Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS644527

Other Notes

Small volumes of anti-ATGL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ATGL purchase

MBS644527 is an antibody targeting ATGL. Also known as ATGL (Patatin-like Phospholipase Domain-containing Protein 2, Adipose Triglyceride Lipase, Calcium-independent Phospholipase A2, Desnutrin, IPLA2-zeta, Pigment Epithelium-derived Factor, TTS2.2, Transport-secretion Protein 2, TTS2, PNPLA2, ATGL, FP17548). Reactive in Bovine, Canine, Human, Mouse, Porcine, Rat. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for ATGL. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
NCBI Official Full Name
ATGL

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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