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5'-Nucleotidase (NT5E) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS644558

CD73 is a 70kD glycophosphatidylinositol (GPI)-linked 5'-nucleotidase, known as ecto-5'-nucleotidase. It converts adenosine monophosphate (AMP) to adenosine. CD73 is expressed on subsets of T- and B-cells; mesenchymal stem cells, follicular dendritic cells,… More details

$785 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
5'-Nucleotidase (Nt5e), Monoclonal Antibody
Full Product Name
5'-Nucleotidase (CD73, CD73 Antigen, Ecto-5'nucleotidase, 5'-NT, eN, eNT, NT5, NT5E, NTE, Purine 5 Prime Nucleotidase)
Product Gene Name
anti-Nt5e antibody
Product Synonym Names
Anti -5'-Nucleotidase (CD73, CD73 Antigen, Ecto-5'nucleotidase, 5'-NT, eN, eNT, NT5, NT5E, NTE, Purine 5 Prime Nucleotidase)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1D7
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid, 0.03% sodium azide.
Purity/Purification
Ascites
Ascites
Specificity
Recognizes human NT5E.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
63,969 Da
Chromosome Location
Chromosome: 8; NC_005107.3 (95464591..95508322). Location: 8q31
3D Structure
ModBase 3D Structure for P21588
Immunogen
Recombinant corresponding to a fragment of human NT5E.

Product Categories/Family for anti-Nt5e antibody

Antibodies; Abs to Enzymes; Nucleotidase
Applications Tested/Suitable for anti-Nt5e antibody
ELISA (EL/EIA), Immunohistochemistry (IHC)
Application Notes for anti-Nt5e antibody
Suitable for use in ELISA and Immunohistochemistry.
Dilution: ELISA: 1:10,000
Immunohistochemistry (Formalin fixed paraffin embedded): 1:200-1:400

Immunohistochemistry (IHC) of anti-Nt5e antibody

Liver, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
Mouse Anti-NT5E Monoclonal Antibody – Cat# MBS644558

Immunohistochemistry (IHC) of anti-Nt5e antibody

Placenta, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
Mouse Anti-NT5E Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS644558

Immunohistochemistry (IHC) of anti-Nt5e antibody

Tonsil, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
Mouse Anti-NT5E Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS644558

Other Notes

Small volumes of anti-Nt5e antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Nt5e purchase

MBS644558 is an antibody targeting Nt5e. Also known as 5'-Nucleotidase (CD73, CD73 Antigen, Ecto-5'nucleotidase, 5'-NT, eN, eNT, NT5, NT5E, NTE, Purine 5 Prime Nucleotidase). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Nt5e. It may not necessarily be applicable to this product.

NCBI GI #
148747417
NCBI GeneID
58813
NCBI Accession #
NP_067587.2
UniProt Primary Accession #
P21588
UniProt Related Accession #
P21588
GenBank Nucleotide #
NM_021576.2
NCBI Official Full Name
5'-nucleotidase
NCBI Official Synonym Full Names
5' nucleotidase, ecto
NCBI Official Symbol
Nt5e
NCBI Official Synonym Symbols
Nt5; CD73
UniProt Protein Name
5'-nucleotidase
UniProt Synonym Protein Names
Ecto-5'-nucleotidase
Protein Family
5'-nucleotidase
UniProt Gene Name
Nt5e
UniProt Synonym Gene Names
Nt5; Nte; 5'-NT
UniProt Entry Name
5NTD_RAT
NCBI Protein Information
5'-nucleotidase; 5'-NT; ecto-5'-nucleotidase

NCBI Summary for Nt5e

may hydrolyze extracellular nucleotides into membrane permeable nucleosides [RGD, Feb 2006]

UniProt Comments for Nt5e

NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5'-nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.3.5; Membrane protein, integral; Nucleotide Metabolism - pyrimidine; Phosphatase (non-protein); Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Membrane protein, GPI anchor; Nucleotide Metabolism - purine

Cellular Component: cell surface; membrane; cytoplasm; integral to membrane; plasma membrane

Molecular Function: 5'-nucleotidase activity; metal ion binding; ferrous iron binding; nucleotide binding

Biological Process: dephosphorylation; adenosine biosynthetic process; negative regulation of inflammatory response; brain development; positive regulation of lipid biosynthetic process; purine nucleotide biosynthetic process; adenosine metabolic process; AMP catabolic process; response to aluminum ion
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Research Articles on Nt5e

1. CD73 is a regulatory factor in osteo-/chondrogenic differentiation of mesenchymal stem cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Nt5e antibody

 Products by Pathway  Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Nucleotides Pathway antibodies  Metabolism Of Nucleotides Pathway Diagram
 Nicotinate And Nicotinamide Metabolism Pathway antibodies  Nicotinate And Nicotinamide Metabolism Pathway Diagram
 Nicotinate And Nicotinamide Metabolism Pathway antibodies  Nicotinate And Nicotinamide Metabolism Pathway Diagram
 Purine Catabolism Pathway antibodies  Purine Catabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Pyrimidine Catabolism Pathway antibodies  Pyrimidine Catabolism Pathway Diagram
 Pyrimidine Metabolism Pathway antibodies  Pyrimidine Metabolism Pathway Diagram

Organs/Tissues associated with anti-Nt5e antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >8 publications with Nt5e and Brain
 Connective Tissue Antibodies  >3 publications with Nt5e and Connective Tissue
 Heart Antibodies  >2 publications with Nt5e and Heart
 Prostate Antibodies  >1 publications with Nt5e and Prostate
 Testis Antibodies  >1 publications with Nt5e and Testis
 Liver Antibodies  >1 publications with Nt5e and Liver
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