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BCLAF1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS644615

This gene encodes a transcriptional repressor that interacts with several members of the BCL2 family of proteins. Overexpression of this protein induces apoptosis, which can be suppressed by co-expression of BCL2 proteins. The protein localizes to dot-like… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
BCLAF1, Polyclonal Antibody
Full Product Name
BCLAF1, CT (BCLAF1, BTF, KIAA0164, Bcl-2-associated transcription factor 1)
Product Gene Name
anti-BCLAF1 antibody
Product Synonym Names
Anti -BCLAF1, CT (BCLAF1, BTF, KIAA0164, Bcl-2-associated transcription factor 1)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
106,122 Da
Chromosome Location
Chromosome: 6; NC_000006.11 (136578001..136611783, complement). Location: 6q22-q23
OMIM
612588
3D Structure
ModBase 3D Structure for Q9NYF8
Immunogen
BCLAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 658-688 amino acids from the C-terminal region of human BCLAF1.

Product Categories/Family for anti-BCLAF1 antibody

Antibodies; Abs to Transcription Factors
Applications Tested/Suitable for anti-BCLAF1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-BCLAF1 antibody
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100

Western Blot (WB) of anti-BCLAF1 antibody

BCLAF1 Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the BCLAF1 antibody detected the BCLAF1 protein (arrow).
Rabbit Anti-BCLAF1 Polyclonal Antibody – Cat# MBS644615

Immunohistochemistry (IHC) of anti-BCLAF1 antibody

BCLAF1 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BCLAF1 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-BCLAF1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS644615

Other Notes

Small volumes of anti-BCLAF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BCLAF1 purchase

MBS644615 is an antibody targeting BCLAF1. Also known as BCLAF1, CT (BCLAF1, BTF, KIAA0164, Bcl-2-associated transcription factor 1). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for BCLAF1. It may not necessarily be applicable to this product.

NCBI GI #
7661958
NCBI GeneID
9774
NCBI Accession #
NP_055554.1
UniProt Primary Accession #
Q9NYF8
UniProt Secondary Accession #
Q14673; Q86WU6; Q86WY0; A2RU75; B7ZM58; E1P586
UniProt Related Accession #
Q9NYF8
GenBank Nucleotide #
NM_014739.2
NCBI Official Full Name
bcl-2-associated transcription factor 1 isoform 1
NCBI Official Synonym Full Names
BCL2-associated transcription factor 1
NCBI Official Symbol
BCLAF1
NCBI Official Synonym Symbols
BTF; bK211L9.1
UniProt Protein Name
Bcl-2-associated transcription factor 1
Protein Family
Bcl-2-associated transcription factor
UniProt Gene Name
BCLAF1
UniProt Synonym Gene Names
BTF; KIAA0164; Btf
UniProt Entry Name
BCLF1_HUMAN
NCBI Protein Information
bcl-2-associated transcription factor 1

UniProt Comments for BCLAF1

BCLAF1: a ubiquitous death-promoting transcriptional repressor. Interacts with Bcl-2 related proteins, lamin A, emerin, the adenovirus E1B 19 kDa protein and DNA. An emerin mutant S54F, which binds normally to barrier-to-autointegration factor (BAF), lamin A and GCL, selectively disrupts its binding to BCLAF1. BCLAF1 is found in dot-like structures throughout the nuclear interior in HeLa cells. Proapoptotic stimuli induce its redistribution to a distinct zone near the nuclear envelope. Four alternatively spliced human isoforms have been described.

Protein type: Transcription factor; RNA-binding

Chromosomal Location of Human Ortholog: 6q23.3

Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; DNA binding

Biological Process: regulation of transcription in response to stress; transcription, DNA-dependent; apoptosis; positive regulation of apoptosis; negative regulation of transcription, DNA-dependent
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Research Articles on BCLAF1

1. BCLAF1 co-localized with gammaH2AX foci in nuclei and stabilized the Ku70/DNA-PKcs complex therein, facilitating non-homologous end joining (NHEJ)-based DSB repair in surviving cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-BCLAF1 antibody

 Reference Product  PubMed Publications
 BCL2L1 antibody  >1 publications with BCLAF1 and BCL2L1

Diseases associated with anti-BCLAF1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >13 publications with BCLAF1 and Neoplasms
 Nervous System Diseases Antibodies  >2 publications with BCLAF1 and Nervous System Diseases
 Necrosis Antibodies  >2 publications with BCLAF1 and Necrosis
 Cardiovascular Diseases Antibodies  >1 publications with BCLAF1 and Cardiovascular Diseases
 Neoplasms, Experimental Antibodies  >1 publications with BCLAF1 and Neoplasms, Experimental
 Ataxia Antibodies  >1 publications with BCLAF1 and Ataxia
 Urinary Bladder Neoplasms Antibodies  >1 publications with BCLAF1 and Urinary Bladder Neoplasms
 Skin Diseases Antibodies  >1 publications with BCLAF1 and Skin Diseases
 Disease Models, Animal Antibodies  >1 publications with BCLAF1 and Disease Models, Animal
 Hyperplasia Antibodies  >1 publications with BCLAF1 and Hyperplasia

Organs/Tissues associated with anti-BCLAF1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >5 publications with BCLAF1 and Blood
 Bone Antibodies  >3 publications with BCLAF1 and Bone
 Lung Antibodies  >3 publications with BCLAF1 and Lung
 Muscle Antibodies  >2 publications with BCLAF1 and Muscle
 Prostate Antibodies  >2 publications with BCLAF1 and Prostate
 Kidney Antibodies  >2 publications with BCLAF1 and Kidney
 Mouth Antibodies  >1 publications with BCLAF1 and Mouth
 Pancreas Antibodies  >1 publications with BCLAF1 and Pancreas
 Nerve Antibodies  >1 publications with BCLAF1 and Nerve
 Heart Antibodies  >1 publications with BCLAF1 and Heart
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