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TTL Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS644679

TTL is a cytosolic enzyme involved in the posttranslational modification of alpha-tubulin (see MIM 602529). Alpha-tubulin within assembled microtubules is detyrosinated over time at the C terminus. After microtubule disassembly, TTL restores the tyrosine… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TTL, Polyclonal Antibody
Full Product Name
TTL, CT (TTL, Tubulin-tyrosine ligase)
Product Gene Name
Product Synonym Names
Anti -TTL, CT (TTL, Tubulin-tyrosine ligase)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
43,212 Da
Chromosome Location
Chromosome: 2; NC_000002.11 (113239743..113290223). Location: 2q13
OMIM
608291
3D Structure
ModBase 3D Structure for Q8NG68
Immunogen
TTL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 355-385 amino acids from the C-terminal region of human TTL.

Product Categories/Family for anti-TTL antibody

Applications Tested/Suitable for anti-TTL antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-TTL antibody
Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500

Western Blot (WB) of anti-TTL antibody

TTL Antibody (C-term) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the TTL antibody detected the TTL protein (arrow).
Rabbit Anti-TTL Polyclonal Antibody – Cat# MBS644679

Other Notes

Small volumes of anti-TTL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TTL purchase

MBS644679 is an antibody targeting TTL. Also known as TTL, CT (TTL, Tubulin-tyrosine ligase). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TTL. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
tubulin--tyrosine ligase
NCBI Official Synonym Full Names
tubulin tyrosine ligase
NCBI Official Symbol
TTL
UniProt Protein Name
Tubulin--tyrosine ligase
Protein Family
UniProt Gene Name
TTL
UniProt Synonym Gene Names
TTL
UniProt Entry Name
TTL_HUMAN
NCBI Protein Information
tubulin--tyrosine ligase; 2410003M22Rik; tubulin-tyrosine ligase

UniProt Comments for TTL

TTL: Catalyzes the post-translational addition of a tyrosine to the C-terminal end of detyrosinated alpha-tubulin. Belongs to the tubulin--tyrosine ligase family.

Protein type: Ligase; Cell development/differentiation; EC 6.3.2.25

Chromosomal Location of Human Ortholog: 2q13

Molecular Function: tubulin-tyrosine ligase activity; ATP binding

Biological Process: protein modification process; microtubule cytoskeleton organization and biogenesis; regulation of axon extension
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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