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DGKZ Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS644740

DGKZ belongs to the eukaryotic diacylglycerol kinase family. It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellular signaling cascade and signal transduction. More details

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Product Overview

Online Datasheet in PDF
Product Name
DGKZ, Polyclonal Antibody
Full Product Name
DGKZ, CT (DGKZ, DAGK6, Diacylglycerol kinase zeta, Diglyceride kinase zeta)
Product Gene Name
anti-DGKZ antibody
Product Synonym Names
Anti -DGKZ, CT (DGKZ, DAGK6, Diacylglycerol kinase zeta, Diglyceride kinase zeta)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
124,128 Da
Chromosome Location
Chromosome: 11; NC_000011.9 (46354455..46402104). Location: 11p11.2
OMIM
601441
3D Structure
ModBase 3D Structure for Q13574
Immunogen
DGKZ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 997~1026 amino acids from the C-terminal region of human DGKZ.

Product Categories/Family for anti-DGKZ antibody

Antibodies; Abs to Protein Kinases
Applications Tested/Suitable for anti-DGKZ antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-DGKZ antibody
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Immunofluorescence

Western Blot (WB) of anti-DGKZ antibody

DGKZ Antibody (D1004) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the DGKZ antibody detected the DGKZ protein (arrow).
Rabbit Anti-DGKZ Polyclonal Antibody – Cat# MBS644740

Western Blot (WB) of anti-DGKZ antibody

Western blot analysis of DGKZ (arrow) using DGKZ Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DGKZ gene (Lane 2) (Origene Technologies).
Rabbit Anti-DGKZ Polyclonal Antibody, Western Blot (WB) – Cat# MBS644740

Immunohistochemistry (IHC) of anti-DGKZ antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-DGKZ Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS644740

Immunofluorescence (IF) of anti-DGKZ antibody

SF1 colocalizes with DGK and PA in the nuclei of H295R cells. Cells were plated onto glass coverslips, fixed, permeabilized, and incubated with anti-SF1 and anti-DGKZ (B) for 1 h. Coverslips were washed and incubated with anti-fluorescein isothiocyanate and antirhodamine, and immunofluorescence was detected by confocal microscopy.
Rabbit Anti-DGKZ Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS644740

Other Notes

Small volumes of anti-DGKZ antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DGKZ purchase

MBS644740 is an antibody targeting DGKZ. Also known as DGKZ, CT (DGKZ, DAGK6, Diacylglycerol kinase zeta, Diglyceride kinase zeta). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DGKZ. It may not necessarily be applicable to this product.

NCBI GI #
313103001
NCBI GeneID
8525
NCBI Accession #
NP_963291.2
UniProt Primary Accession #
Q13574
UniProt Secondary Accession #
O00542; Q6ZVG7; Q8IVW9; B7Z2M9; E9PPW4; G3V0F6; J3KNJ6
UniProt Related Accession #
Q13574
GenBank Nucleotide #
NM_201533.3
NCBI Official Full Name
diacylglycerol kinase zeta isoform 3
NCBI Official Synonym Full Names
diacylglycerol kinase, zeta
NCBI Official Symbol
DGKZ
NCBI Official Synonym Symbols
DAGK5; DAGK6; DGK-ZETA; hDGKzeta
UniProt Protein Name
Diacylglycerol kinase zeta
UniProt Synonym Protein Names
Diglyceride kinase zeta
Protein Family
Diacylglycerol kinase
UniProt Gene Name
DGKZ
UniProt Synonym Gene Names
DAGK6; DAG kinase zeta; DGK-zeta
UniProt Entry Name
DGKZ_HUMAN
NCBI Protein Information
diacylglycerol kinase zeta; DAG kinase zeta; diglyceride kinase zeta; diacylglycerol kinase, zeta 104kDa

NCBI Summary for DGKZ

The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellular signaling cascade and signal transduction. Alternative splicing occurs at this locus and multiple transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Nov 2010]
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UniProt Comments for DGKZ

DGKZ: Displays a strong preference for 1,2-diacylglycerols over 1,3-diacylglycerols, but lacks substrate specificity among molecular species of long chain diacylglycerols. Isoform 2 but not isoform 1 regulates RASGRP1 activity. Belongs to the eukaryotic diacylglycerol kinase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - glycerolipid; EC 2.7.1.107; Kinase, lipid; Motility/polarity/chemotaxis; Lipid Metabolism - glycerophospholipid

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: nucleoplasm; lamellipodium; cytoplasm; plasma membrane; nucleus

Molecular Function: protein C-terminus binding; enzyme inhibitor activity; lipid kinase activity; protein binding; metal ion binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity

Biological Process: G1 DNA damage checkpoint; platelet activation; cell migration; protein kinase C activation; negative regulation of catalytic activity; negative regulation of Ras protein signal transduction; negative regulation of mitotic cell cycle; blood coagulation; lipid phosphorylation; phosphorylation
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Research Articles on DGKZ

1. Data indicate that after P2Y6 receptor stimulation both phospholipase D (PLD) and DGKzeta enzymes are responsible for producing phosphatidic acid (PA).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-DGKZ antibody

 Products by Pathway  Pathway Diagram
 Effects Of PIP2 Hydrolysis Pathway antibodies  Effects Of PIP2 Hydrolysis Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 Glycerolipid Metabolism Pathway antibodies  Glycerolipid Metabolism Pathway Diagram
 Glycerolipid Metabolism Pathway antibodies  Glycerolipid Metabolism Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Myometrial Relaxation And Contraction Pathways antibodies  Myometrial Relaxation And Contraction Pathways Diagram

Diseases associated with anti-DGKZ antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >1 publications with DGKZ and Neoplasms

Organs/Tissues associated with anti-DGKZ antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >2 publications with DGKZ and Brain
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