• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

PROX-1 (PROX1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS644835

Apolipoprotein H has been implicated in a variety of physiologic pathways including lipoprotein metabolism, coagulation, and the production of antiphospholipid autoantibodies. APOH may be a required cofactor for anionic phospholipid binding by the… More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PROX-1 (PROX1), Polyclonal Antibody
Full Product Name
PROX-1 (S514) (PROX1, Prospero homeobox protein 1, Homeobox prospero-like protein PROX1)
Product Gene Name
Product Synonym Names
Anti -PROX-1 (S514) (PROX1, Prospero homeobox protein 1, Homeobox prospero-like protein PROX1)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human, mouse
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
83,141 Da
Chromosome Location
Chromosome: 3; NC_006090.3 (20626242..20662934, complement). Location: chromosome: 3
3D Structure
ModBase 3D Structure for Q91018
Immunogen
PROX-1-S514 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acid 500-530 from the region of human PROX.
Applications Tested/Suitable for anti-PROX1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-PROX1 antibody
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-PROX1 antibody

Western Blot analysis in 293 cell line lysates (35ug/lane) using MBS644835. PROX-1 (arrow) was detected using MBS644835.
Rabbit Anti-PROX1 Polyclonal Antibody – Cat# MBS644835

Western Blot (WB) of anti-PROX1 antibody

Western Blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane) using MBS644835. PROX-1 (arrow) was detected using MBS644835.
Rabbit Anti-PROX1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS644835

Testing Data of anti-PROX1 antibody

All lanes: 044084 (1:2000) Lane 1: HepG2 whole cell lysate (20ug/lane) Lane 2: SH-SY5Y whole cell lysate (20ug/lane) Lane 3: SW620 whole cell lysate (20ug/lane). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution. Predicted band size: 83kD Blocking/Dilution buffer: 5% NFDM/TBST.
Rabbit Anti-PROX1 Polyclonal Antibody, Testing Data – Cat# MBS644835

Immunohistochemistry (IHC) of anti-PROX1 antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue using MBS644835 followed by peroxidase conjugation of the secondary antibody and DAB staining.
Rabbit Anti-PROX1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS644835

Flow Cytometry (FC/FACS) of anti-PROX1 antibody

Flow Cytometric analysis of 293 cells using MBS644835 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-PROX1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS644835

Other Notes

Small volumes of anti-PROX1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PROX1 purchase

MBS644835 is an antibody targeting PROX1. Also known as PROX-1 (S514) (PROX1, Prospero homeobox protein 1, Homeobox prospero-like protein PROX1). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PROX1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
prospero homeobox protein 1
NCBI Official Synonym Full Names
prospero homeobox 1
NCBI Official Symbol
PROX1
UniProt Protein Name
Prospero homeobox protein 1
UniProt Synonym Protein Names
Homeobox prospero-like protein PROX1
UniProt Gene Name
PROX1
UniProt Synonym Gene Names
PROX-1
UniProt Entry Name
PROX1_CHICK
NCBI Protein Information
prospero homeobox protein 1; PROX-1; prospero-related homeobox 1; homeobox prospero-like protein PROX1

UniProt Comments for PROX1

Function: May be essential in the development and function of the eye.

Subcellular location: Nucleus

Probable.

Tissue specificity: Expressed most actively in the developing lens and midgut and at lower levels in the developing brain, heart, muscle and retina.

Developmental stage: First detected at stage 14 in the early lens placode. At later stages of development, it was observed throughout the lens, but it appeared more abundant around the bow region of the equator than in the anterior epithelium or the fibers. In the retina, expression was detected mainly in the inner nuclear layer during later stages of histogenesis.

Sequence similarities: Belongs to the Prospero homeobox family.Contains 1 Prospero-type homeobox DNA-binding domain.
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PROX1 antibody

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000