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RPA4 Antibody – Mouse Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS644897

MBS644897 is an antibody targeting RPA4. Also known as RPA4 (Replication Factor A Protein 4, RF-A Protein 4, Replication Protein A 30 kDa Subunit, RP-A p30, HSU24186). Reactive in Human. Host: Mouse. More details

$880 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
RPA4, Polyclonal Antibody
Full Product Name
RPA4 (Replication Factor A Protein 4, RF-A Protein 4, Replication Protein A 30 kDa Subunit, RP-A p30, HSU24186)
Product Gene Name
anti-RPA4 antibody
Product Synonym Names
Anti -RPA4 (Replication Factor A Protein 4, RF-A Protein 4, Replication Protein A 30 kDa Subunit, RP-A p30, HSU24186)
Immunogen Sequence
MSKSGFGSYG SISAADGASG GSDQLCERDA TPAIKTQRPK VRIQDVVPCN VNQLLSSTVF DPVFKVRGII VSQVSIVGVI RGAEKASNHI CYKIDDMTAK PIEARQWFGR EKVKQVTPLS VGVYVKVFGI LKCPTGTKSL EVLKIHVLED MNEFTVHILE TVNAHMMLDK ARRDTTVESV PVSPSEVNDA GDNDESHRNF IQDEVLRLIH ECPHQEGKSI HELRAQLCDL SVKAIKEAID YLTVEGHIYP TVDREHFKSA D
Clonality
Polyclonal
Isotype
IgG
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human RPA4.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
28,868 Da
Chromosome Location
Chromosome: X; NC_000023.10 (96138907..96140466). Location: Xq21.33
OMIM
300767
3D Structure
ModBase 3D Structure for Q13156
Immunogen
Full length human RPA4, aa1-261 (NP_037479.1).

Product Categories/Family for anti-RPA4 antibody

Antibodies; Abs to DNA Replication; Repair
Applications Tested/Suitable for anti-RPA4 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-RPA4 antibody
Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
Dilution: Immunofluorescence: 10ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml

Western Blot (WB) of anti-RPA4 antibody

RPA4 polyclonal antibody. Western Blot analysis of RPA4 expression in human spleen.
Mouse Anti-RPA4 Polyclonal Antibody – Cat# MBS644897

Western Blot (WB) of anti-RPA4 antibody

Western Blot analysis of RPA4 expression in transfected 293T cell line by RPA4 polyclonal antibody. Lane 1: RPA4 transfected lysate (28.71kD). Lane 2: Non-transfected lysate.
Mouse Anti-RPA4 Polyclonal Antibody, Western Blot (WB) – Cat# MBS644897

Immunohistochemistry (IHC) of anti-RPA4 antibody

Immunoperoxidase of purified antibody to RPA4 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml].
Mouse Anti-RPA4 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS644897

Immunofluorescence (IF) of anti-RPA4 antibody

Immunofluorescence of purified antibody to RPA4 on HeLa cell. [antibody concentration 10ug/ml].
Mouse Anti-RPA4 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS644897

Other Notes

Small volumes of anti-RPA4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RPA4. It may not necessarily be applicable to this product.

NCBI GI #
9558731
NCBI GeneID
29935
NCBI Accession #
NP_037479.1
UniProt Primary Accession #
Q13156
UniProt Secondary Accession #
Q3SY03
UniProt Related Accession #
Q13156
GenBank Nucleotide #
NM_013347.4
NCBI Official Full Name
replication protein A 30 kDa subunit
NCBI Official Synonym Full Names
replication protein A4, 30kDa
NCBI Official Symbol
RPA4
NCBI Official Synonym Symbols
HSU24186
UniProt Protein Name
Replication protein A 30 kDa subunit
UniProt Synonym Protein Names
Replication factor A protein 4
Protein Family
Replication protein
UniProt Gene Name
RPA4
UniProt Synonym Gene Names
RP-A p30
UniProt Entry Name
RFA4_HUMAN
NCBI Protein Information
replication protein A 30 kDa subunit; RP-A p30; RF-A protein 4; replication protein A4, 34kDa; replication factor A protein 4; replication protein A complex 34 kd subunit homolog Rpa4

NCBI Summary for RPA4

This gene encodes a single-stranded DNA-binding protein that is the 30-kDa subunit of the replication protein A complex. Replication protein A is an essential factor for DNA double-strand break repair and cell cycle checkpoint activation. The encoded protein localizes to DNA repair foci and may be involved in the cellular DNA damage response. This protein may also play a role in inhibiting viral replication.[provided by RefSeq, Apr 2010]
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UniProt Comments for RPA4

RPA4: functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange. Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. Interacts with RPA1 and RPA3. Localizes to DNA repair foci after DNA damage.

Chromosomal Location of Human Ortholog: Xq21.33

Cellular Component: nucleoplasm; DNA replication factor A complex; nucleus

Molecular Function: single-stranded DNA binding

Biological Process: DNA replication initiation; nucleotide-excision repair; DNA damage checkpoint; DNA replication during S phase; mitotic cell cycle; G1/S transition of mitotic cell cycle; DNA recombination
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Research Articles on RPA4

1. Findings provide the first direct evidence for the function of alternative form of RPA in human DNA metabolism and support a model for aRPA functioning in chromosome maintenance functions in nonproliferating cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RPA4 antibody

 Reference Product  PubMed Publications
 RPA3 antibody  >6 publications with RPA4 and RPA3
 RPA1 antibody  >4 publications with RPA4 and RPA1

Pathways associated with anti-RPA4 antibody

 Products by Pathway  Pathway Diagram
 Activation Of The Pre-replicative Complex Pathway antibodies  Activation Of The Pre-replicative Complex Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 DNA Replication Pre-Initiation Pathway antibodies  DNA Replication Pre-Initiation Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 Fanconi Anemia Pathway antibodies  Fanconi Anemia Pathway Diagram
 Fanconi Anemia Pathway antibodies  Fanconi Anemia Pathway Diagram

Diseases associated with anti-RPA4 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >2 publications with RPA4 and Neoplasms
 Poisoning Antibodies  >2 publications with RPA4 and Poisoning
 Nervous System Diseases Antibodies  >1 publications with RPA4 and Nervous System Diseases
 Brain Diseases Antibodies  >1 publications with RPA4 and Brain Diseases
 Lymphadenitis Antibodies  >1 publications with RPA4 and Lymphadenitis
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