• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

CHCHD4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS644993

CHCHD4, a component of human mitochondria, belongs to a protein family whose members share 6 highly conserved cysteine residues constituting a -CXC-CX(9)C-CX(9)C- motif in the C terminus (Hofmann et al., 2005 [PubMed 16185709]). More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MIA40 (CHCHD4), Polyclonal Antibody
Full Product Name
MIA40, CT (CHCHD4, MIA40, Mitochondrial intermembrane space import and assembly protein 40, Coiled-coil-helix-coiled-coil-helix domain-containing protein 4)
Product Gene Name
anti-CHCHD4 antibody
Product Synonym Names
Anti -MIA40, CT (CHCHD4, MIA40, Mitochondrial intermembrane space import and assembly protein 40, Coiled-coil-helix-coiled-coil-helix domain-containing protein 4)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
15,996 Da
Chromosome Location
Chromosome: 3; NC_000003.11 (14153577..14166371, complement). Location: 3p25.1
OMIM
611077
3D Structure
ModBase 3D Structure for Q8N4Q1
Immunogen
MIA40 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-137 amino acids from the C-terminal region of human MIA40.

Product Categories/Family for anti-CHCHD4 antibody

Antibodies; Abs to Mitochondrial Proteins
Applications Tested/Suitable for anti-CHCHD4 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-CHCHD4 antibody
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50

Western Blot (WB) of anti-CHCHD4 antibody

MIA40 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the MIA40 antibody detected the MIA40 protein (arrow).
Rabbit Anti-CHCHD4 Polyclonal Antibody – Cat# MBS644993

Immunohistochemistry (IHC) of anti-CHCHD4 antibody

MIA40 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MIA40 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-CHCHD4 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS644993

Other Notes

Small volumes of anti-CHCHD4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CHCHD4 purchase

MBS644993 is an antibody targeting CHCHD4. Also known as MIA40, CT (CHCHD4, MIA40, Mitochondrial intermembrane space import and assembly protein 40, Coiled-coil-helix-coiled-coil-helix domain-containing protein 4). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CHCHD4. It may not necessarily be applicable to this product.

NCBI GI #
148612859
NCBI GeneID
131474
NCBI Accession #
NP_001091972.1
UniProt Primary Accession #
Q8N4Q1
UniProt Secondary Accession #
Q96AI2; Q96MY6; A8K3Z9
UniProt Related Accession #
Q8N4Q1
GenBank Nucleotide #
NM_001098502.1
NCBI Official Full Name
mitochondrial intermembrane space import and assembly protein 40 isoform 1
NCBI Official Synonym Full Names
coiled-coil-helix-coiled-coil-helix domain containing 4
NCBI Official Symbol
CHCHD4
NCBI Official Synonym Symbols
MIA40; TIMM40
UniProt Protein Name
Mitochondrial intermembrane space import and assembly protein 40
UniProt Synonym Protein Names
Coiled-coil-helix-coiled-coil-helix domain-containing protein 4
Protein Family
Mitochondrial intermembrane space import and assembly protein
UniProt Gene Name
CHCHD4
UniProt Synonym Gene Names
MIA40
UniProt Entry Name
MIA40_HUMAN
NCBI Protein Information
mitochondrial intermembrane space import and assembly protein 40; translocase of inner mitochondrial membrane 40 homolog; coiled-coil-helix-coiled-coil-helix domain-containing protein 4; mitochondrial intermembrane space import and assembly 40 homolog

UniProt Comments for CHCHD4

CHCHD4: Required for the import and folding of small cysteine- containing proteins (small Tim) in the mitochondrial intermembrane space (IMS). Probably acts by forming a redox cycle with GFER/ERV1 that involves a disulfide relay system. Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria. The oxidized form of MIA40 forms a transient intermolecular disulfide bridge with the reduced precursor protein, resulting in oxidation of the precursor protein that now contains an intramolecular disulfide bond and is able to undergo folding in the IMS (Probable). 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 3p25.1

Cellular Component: mitochondrion; mitochondrial intermembrane space

Molecular Function: protein disulfide oxidoreductase activity

Biological Process: 'de novo' posttranslational protein folding; protein maturation via protein folding; cellular protein metabolic process; protein import into mitochondrial intermembrane space; protein targeting to mitochondrion
Show more

Research Articles on CHCHD4

1. mitochondrial localization of MIA40 requires sulfhydryl oxidase ALR in heterologous expression yeast system.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CHCHD4 antibody

 Products by Pathway  Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Mitochondrial Protein Import Pathway antibodies  Mitochondrial Protein Import Pathway Diagram

Organs/Tissues associated with anti-CHCHD4 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >1 publications with CHCHD4 and Lung
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000