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EXTL3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS645103

MBS645103 is an antibody targeting EXTL3. Also known as EXTL3, NT (EXTL3, EXTL1L, EXTR1, KIAA0519, Exostosin-like 3, EXT-related protein 1, Glucuronyl-galactosyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase, Hereditary multiple exostoses gene isolog,… More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
EXTL3, Polyclonal Antibody
Full Product Name
EXTL3, NT (EXTL3, EXTL1L, EXTR1, KIAA0519, Exostosin-like 3, EXT-related protein 1, Glucuronyl-galactosyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase, Hereditary multiple exostoses gene isolog, Multiple exostosis-like protein 3, Putative tumor su
Product Gene Name
Product Synonym Names
Anti -EXTL3, NT (EXTL3, EXTL1L, EXTR1, KIAA0519, Exostosin-like 3, EXT-related protein 1, Glucuronyl-galactosyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase, Hereditary multiple exostoses gene isolog, Multiple exostosis-like protein 3, Putative tumor su
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
104,749 Da
Chromosome Location
Chromosome: 8; NC_000008.10 (28558990..28611207). Location: 8p21
OMIM
605744
3D Structure
ModBase 3D Structure for O43909
Immunogen
EXTL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50~80 amino acids from the N-terminal region of human EXTL3.
Applications Tested/Suitable for anti-EXTL3 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-EXTL3 antibody
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-EXTL3 antibody

Western blot analysis of EXTL3 Antibody (N-term) in MDA-MB435, MDA-MB468 cell line lysates (35ug/lane). EXTL3 (arrow) was detected using the purified Pab.
Rabbit Anti-EXTL3 Polyclonal Antibody – Cat# MBS645103

Immunohistochemistry (IHC) of anti-EXTL3 antibody

EXTL3 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EXTL3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-EXTL3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS645103

Flow Cytometry (FC/FACS) of anti-EXTL3 antibody

EXTL3 Antibody (N-term) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-EXTL3 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS645103

Other Notes

Small volumes of anti-EXTL3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for EXTL3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
exostosin-like 3
NCBI Official Synonym Full Names
exostosin-like glycosyltransferase 3
NCBI Official Symbol
EXTL3
NCBI Official Synonym Symbols
RPR; BOTV; REGR; EXTR1; EXTL1L
UniProt Protein Name
Exostosin-like 3
UniProt Synonym Protein Names
EXT-related protein 1; Glucuronyl-galactosyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase; Hereditary multiple exostoses gene isolog; Multiple exostosis-like protein 3; Putative tumor suppressor protein EXTL3
Protein Family
UniProt Gene Name
EXTL3
UniProt Synonym Gene Names
EXTL1L; EXTR1; KIAA0519
UniProt Entry Name
EXTL3_HUMAN
NCBI Protein Information
exostosin-like 3; reg receptor; EXT-related 1; exostosin tumor-like 3; exostoses (multiple)-like 3; putative tumor suppressor protein EXTL3; hereditary multiple exostoses gene isolog; glucuronyl-galactosyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase

NCBI Summary for EXTL3

This gene encodes a single-pass membrane protein which functions as a glycosyltransferase. The encoded protein catalyzes the transfer of N-acetylglucosamine to glycosaminoglycan chains. This reaction is important in heparin and heparan sulfate synthesis. Alternative splicing results in the multiple transcript variants. [provided by RefSeq, Nov 2012]
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UniProt Comments for EXTL3

EXTL3: Probable glycosyltransferase. Belongs to the glycosyltransferase 47 family.

Protein type: Membrane protein, integral; EC 2.4.1.223; Transferase; Glycan Metabolism - heparan sulfate biosynthesis

Chromosomal Location of Human Ortholog: 8p21

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane

Molecular Function: metal ion binding; glucuronyl-galactosyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity

Biological Process: glycosaminoglycan biosynthetic process; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; unfolded protein response; heparan sulfate proteoglycan biosynthetic process; positive regulation of cell growth
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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