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HSPA5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS645158

When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to… More details

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Product Overview

Online Datasheet in PDF
Product Name
HSPA5, Polyclonal Antibody
Full Product Name
HSPA5, CT (HSPA5, GRP78, 78kD glucose-regulated protein, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Heat shock 70kD protein 5, Immunoglobulin heavy chain-binding protein)
Product Gene Name
anti-HSPA5 antibody
Product Synonym Names
Anti -HSPA5, CT (HSPA5, GRP78, 78kD glucose-regulated protein, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Heat shock 70kD protein 5, Immunoglobulin heavy chain-binding protein)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
72,333 Da
Chromosome Location
Chromosome: 9; NC_000009.11 (127997127..128003666, complement). Location: 9q33.3
OMIM
138120
3D Structure
ModBase 3D Structure for P11021
Immunogen
HSPA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 548-587 amino acids from the C-terminal region of human HSPA5.

Product Categories/Family for anti-HSPA5 antibody

Antibodies; Abs to Heat Shock Proteins
Applications Tested/Suitable for anti-HSPA5 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-HSPA5 antibody
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-HSPA5 antibody

Western Blot analysis in A375, A2058, Hela cell line lysates (35ug/lane) using MBS645158. This demonstrates that MBS645158 detected the HSPA5 protein (arrow).
Rabbit Anti-HSPA5 Polyclonal Antibody – Cat# MBS645158

Immunohistochemistry (IHC) of anti-HSPA5 antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma using MBS645158 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS645158 for immunohistochemistry.
Rabbit Anti-HSPA5 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS645158

Flow Cytometry (FC/FACS) of anti-HSPA5 antibody

Flow Cytometric analysis of Hela cells using MBS645158 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-HSPA5 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS645158

Other Notes

Small volumes of anti-HSPA5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HSPA5 purchase

MBS645158 is an antibody targeting HSPA5. Also known as HSPA5, CT (HSPA5, GRP78, 78kD glucose-regulated protein, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Heat shock 70kD protein 5, Immunoglobulin heavy chain-binding protein). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for HSPA5. It may not necessarily be applicable to this product.

NCBI GI #
86577744
NCBI GeneID
3309
NCBI Accession #
AAI12964.1
UniProt Primary Accession #
P11021
UniProt Secondary Accession #
Q2EF78; Q9NPF1; Q9UK02; B0QZ61
UniProt Related Accession #
P11021
NCBI Official Full Name
HSPA5 protein
NCBI Official Synonym Full Names
heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
NCBI Official Symbol
HSPA5
NCBI Official Synonym Symbols
BIP; MIF2; GRP78
UniProt Protein Name
78 kDa glucose-regulated protein
UniProt Synonym Protein Names
Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein
Protein Family
Transmembrane protein
UniProt Gene Name
HSPA5
UniProt Synonym Gene Names
GRP78; GRP-78; BiP
UniProt Entry Name
GRP78_HUMAN
NCBI Protein Information
78 kDa glucose-regulated protein; immunoglobulin heavy chain-binding protein; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78

NCBI Summary for HSPA5

The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.[provided by RefSeq, Sep 2010]
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UniProt Comments for HSPA5

GRP78: a member of the HSP family of molecular chaperones required for endoplasmic reticulum integrity and stress-induced autophagy. Plays a central role in regulating the unfolded protein response (UPR), and is an obligatory component of autophagy in mammalian cells. May play an important role in cellular adaptation and oncogenic survival. One of the client proteins of GRP78 is protein double-stranded RNA-activated protein-like endoplasmic reticulum kinase (PERK). Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.

Protein type: Heat shock protein; Chaperone

Chromosomal Location of Human Ortholog: 9q33.3

Cellular Component: signalosome; endoplasmic reticulum membrane; cell surface; focal adhesion; smooth endoplasmic reticulum; mitochondrion; endoplasmic reticulum; endoplasmic reticulum lumen; ER-Golgi intermediate compartment; membrane; plasma membrane; melanosome; integral to endoplasmic reticulum membrane; midbody; nucleus

Molecular Function: protein domain specific binding; protein binding; enzyme binding; chaperone binding; ubiquitin protein ligase binding; unfolded protein binding; ATPase activity; ribosome binding; misfolded protein binding; calcium ion binding; glycoprotein binding; ATP binding

Biological Process: platelet activation; ER-associated protein catabolic process; cerebellum structural organization; unfolded protein response; cerebellar Purkinje cell layer development; cellular response to glucose starvation; cellular protein metabolic process; platelet degranulation; substantia nigra development; unfolded protein response, activation of signaling protein activity; positive regulation of protein ubiquitination; negative regulation of transforming growth factor beta receptor signaling pathway; blood coagulation; ER overload response; positive regulation of cell migration; negative regulation of apoptosis
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Research Articles on HSPA5

1. BiP was particularly observed in the pathological vasculature and retinal microvascular endothelial cells, and the increase of BiP expression was correlated with retinal neovascularization.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HSPA5 antibody

 Reference Product  PubMed Publications
 ATF6 antibody  >24 publications with HSPA5 and ATF6
 ATF4 antibody  >21 publications with HSPA5 and ATF4
 SIL1 antibody  >4 publications with HSPA5 and SIL1
 ERN1 antibody  >4 publications with HSPA5 and ERN1
 EIF2AK3 antibody  >2 publications with HSPA5 and EIF2AK3
 NFYA antibody  >1 publications with HSPA5 and NFYA

Pathways associated with anti-HSPA5 antibody

 Products by Pathway  Pathway Diagram
 Activation Of Chaperone Genes By ATF6-alpha Pathway antibodies  Activation Of Chaperone Genes By ATF6-alpha Pathway Diagram
 Activation Of Chaperones By ATF6-alpha Pathway antibodies  Activation Of Chaperones By ATF6-alpha Pathway Diagram
 Activation Of Chaperones By IRE1alpha Pathway antibodies  Activation Of Chaperones By IRE1alpha Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway antibodies  Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram

Diseases associated with anti-HSPA5 antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >53 publications with HSPA5 and Cardiovascular Diseases
 Nervous System Diseases Antibodies  >50 publications with HSPA5 and Nervous System Diseases
 Disease Models, Animal Antibodies  >44 publications with HSPA5 and Disease Models, Animal
 Brain Diseases Antibodies  >43 publications with HSPA5 and Brain Diseases
 Neoplasms Antibodies  >31 publications with HSPA5 and Neoplasms
 Inflammation Antibodies  >21 publications with HSPA5 and Inflammation
 Heart Diseases Antibodies  >19 publications with HSPA5 and Heart Diseases
 Liver Diseases Antibodies  >16 publications with HSPA5 and Liver Diseases
 Necrosis Antibodies  >15 publications with HSPA5 and Necrosis
 Kidney Diseases Antibodies  >13 publications with HSPA5 and Kidney Diseases

Organs/Tissues associated with anti-HSPA5 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >67 publications with HSPA5 and Brain
 Muscle Antibodies  >41 publications with HSPA5 and Muscle
 Blood Antibodies  >34 publications with HSPA5 and Blood
 Heart Antibodies  >32 publications with HSPA5 and Heart
 Liver Antibodies  >29 publications with HSPA5 and Liver
 Kidney Antibodies  >27 publications with HSPA5 and Kidney
 Nerve Antibodies  >18 publications with HSPA5 and Nerve
 Vascular Antibodies  >18 publications with HSPA5 and Vascular
 Pancreas Antibodies  >13 publications with HSPA5 and Pancreas
 Testis Antibodies  >11 publications with HSPA5 and Testis
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