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RPC31 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS645223

RPC8 is DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. It is… More details

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Product Overview

Online Datasheet in PDF
Product Name
RPC8 (RPC31), Polyclonal Antibody
Full Product Name
RPC8, NT (POLR3H, KIAA1665, RPC8, DNA-directed RNA polymerase III subunit RPC8, DNA-directed RNA polymerase III subunit H, RNA polymerase III subunit 22.9kD subunit)
Product Gene Name
Product Synonym Names
Anti -RPC8, NT (POLR3H, KIAA1665, RPC8, DNA-directed RNA polymerase III subunit RPC8, DNA-directed RNA polymerase III subunit H, RNA polymerase III subunit 22.9kD subunit)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human, mouse
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
27,724 Da
Chromosome Location
Chromosome: XIV; NC_001146.8 (347766..348521, complement). Location: chromosome: XIV
3D Structure
ModBase 3D Structure for P17890
Immunogen
RPC8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acid 10-40 from the N-terminal region of human RPC8.

Product Categories/Family for anti-RPC31 antibody

Applications Tested/Suitable for anti-RPC31 antibody
ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-RPC31 antibody
Suitable for use in Western Blot, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-RPC31 antibody

Western blot analysis of RPC8 Antibody (N-term) in mouse testis tissue lysates (35ug/lane). RPC8 (arrow) was detected using the purified Pab.
Rabbit Anti-RPC31 Polyclonal Antibody – Cat# MBS645223

Flow Cytometry (FC/FACS) of anti-RPC31 antibody

RPC8 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-RPC31 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS645223

Other Notes

Small volumes of anti-RPC31 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RPC31 purchase

MBS645223 is an antibody targeting RPC31. Also known as RPC8, NT (POLR3H, KIAA1665, RPC8, DNA-directed RNA polymerase III subunit RPC8, DNA-directed RNA polymerase III subunit H, RNA polymerase III subunit 22.9kD subunit). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RPC31. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
Rpc31p
NCBI Official Symbol
RPC31
NCBI Official Synonym Symbols
ACP2; RPC8
UniProt Protein Name
DNA-directed RNA polymerase III subunit RPC7
UniProt Synonym Protein Names
DNA-directed RNA polymerase III 31 kDa polypeptide
UniProt Gene Name
RPC31
UniProt Synonym Gene Names
ACP2; RPC8; RNA polymerase III subunit C7; C31
UniProt Entry Name
RPC7_YEAST
NCBI Protein Information
Rpc31p

UniProt Comments for RPC31

Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. C31 is involved in the formation of the initiation complex.

Subunit structure: Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.

Subcellular location: Nucleus.

Miscellaneous: The acidic domain is essential for its function.

Sequence similarities: Belongs to the eukaryotic RPC7 RNA polymerase subunit family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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