Mouse Anti-DOHH Polyclonal Antibody – Cat# MBS645239

Western Blot analysis of HLRC1 expression in transfected 293T cell line by HLRC1 polyclonal antibody. Lane 1: HLRC1 transfected lysate (33.22kD). Lane 2: Non-transfected lysate.

HLRC1 (DOHH) Antibody – Mouse Polyclonal

Name: DOHH Antibody; Polyclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS645239
Price: 880 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS645239

Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor. More details

$880 / 0.05 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

HLRC1 (DOHH), Polyclonal Antibody

Full Product Name

HLRC1 (Deoxyhypusine Hydroxylase, hDOHH, Deoxyhypusine Dioxygenase, Deoxyhypusine Monooxygenase, HEAT-like Repeat-containing Protein 1, DOHH, MGC4293)

Product Gene Name

anti-DOHH antibody

Product Synonym Names

Anti -HLRC1 (Deoxyhypusine Hydroxylase, hDOHH, Deoxyhypusine Dioxygenase, Deoxyhypusine Monooxygenase, HEAT-like Repeat-containing Protein 1, DOHH, MGC4293)

Immunogen Sequence

MVTEQEVDAI GQTLVDPKQP LQARFRALFT LRGLGGPGAI AWISQAFDDD SALLKHELAY CLGQMQDARA IPMLVDVLQD TRQEPMVRHE AGEALGAIGD PEVLEILKQY SSDPVIEVAE TCQLAVRRLE WLQQHGGEPA AGPYLSVDPA PPAEERDVGR LREALLDESR PLFERYRAMF ALRNAGGEEA ALALAEGLHC GSALFRHEVG YVLGQLQHEA AVPQLAAALA RCTENPMVRH ECAEALGAIA RPACLAALQA HADDPERVVR ESCEVALDMY EHETGRAFQY ADGLEQLRGA PS

Clonality

Polyclonal

Isotype

IgG

Host

Mouse

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2.

Purity/Purification

Affinity Purified
Purified by Protein A affinity chromatography.

Specificity

Recognizes human HLRC1.

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

32,904 Da

Chromosome Location

Chromosome: 19; NC_000019.9 (3490819..3500938, complement). Location: 19p13.3

OMIM

611262

3D Structure

ModBase 3D Structure for Q9BU89

Immunogen

Full length human HLRC1, aa1-302 (NP_112594).

Product Categories/Family for anti-DOHH antibody

Antibodies; Abs to Enzymes

Applications Tested/Suitable for anti-DOHH antibody

Western Blot (WB)

Application Notes for anti-DOHH antibody

Suitable for use in Western Blot.

Other Notes

Small volumes of anti-DOHH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DOHH purchase

MBS645239 is an antibody targeting DOHH. Also known as HLRC1 (Deoxyhypusine Hydroxylase, hDOHH, Deoxyhypusine Dioxygenase, Deoxyhypusine Monooxygenase, HEAT-like Repeat-containing Protein 1, DOHH, MGC4293). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for DOHH. It may not necessarily be applicable to this product.

NCBI GI #

119589723

NCBI GeneID

83475

NCBI Accession #

EAW69317.1

UniProt Primary Accession #

Q9BU89

UniProt Secondary Accession #

O75265

UniProt Related Accession #

Q9BU89

NCBI Official Full Name

HEAT-like (PBS lyase) repeat containing 1, isoform CRA_b

NCBI Official Synonym Full Names

deoxyhypusine hydroxylase/monooxygenase

NCBI Official Symbol

DOHH

NCBI Official Synonym Symbols

HLRC1; hDOHH

UniProt Protein Name

Deoxyhypusine hydroxylase

UniProt Synonym Protein Names

Deoxyhypusine dioxygenase; Deoxyhypusine monooxygenase; HEAT-like repeat-containing protein 1

Protein Family

Deoxyhypusine hydroxylase

UniProt Gene Name

DOHH

UniProt Synonym Gene Names

HLRC1; hDOHH

UniProt Entry Name

DOHH_HUMAN

NCBI Protein Information

deoxyhypusine hydroxylase; deoxyhypusine dioxygenase; deoxyhypusine monooxygenase; HEAT-like repeat-containing protein 1; HEAT-like (PBS lyase) repeat containing 1

NCBI Summary for DOHH

This gene encodes a metalloenzyme that catalyzes the last step in the conversion of lysine to the unique amino acid hypusine in eukaryotic initiation factor 5A. The encoded protein hydroxylates deoxyhypusine to form hypusine in the mature eukaryotic initiation factor 5A protein. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2009]

UniProt Comments for DOHH

DOHH: Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)- L-lysine intermediate to form hypusine, an essential post- translational modification only found in mature eIF-5A factor. Belongs to the deoxyhypusine hydroxylase family.

Protein type: EC 1.14.99.29; Oxidoreductase

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cytosol

Molecular Function: protein binding; metal ion binding; deoxyhypusine monooxygenase activity

Biological Process: cellular protein metabolic process; hypusine biosynthetic process from peptidyl-lysine; post-translational protein modification

Research Articles on DOHH

1. eIF-5A as well as the hypusine-forming enzymes deoxyhypusine synthase (DHS) and deoxyhypusine hydroxylase (DOHH) are highly overexpressed in glioblastoma patient sample

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DOHH antibody

Reference Product	PubMed Publications
DHPS antibody	>1 publications with DOHH and DHPS

Pathways associated with anti-DOHH antibody

Products by Pathway	Pathway Diagram
Hypusine Synthesis From EIF5A-lysine Pathway antibodies	Hypusine Synthesis From EIF5A-lysine Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram
PTM: Gamma Carboxylation, Hypusine Formation And Arylsulfatase Activation Pathway antibodies	PTM: Gamma Carboxylation, Hypusine Formation And Arylsulfatase Activation Pathway Diagram
Post-translational Protein Modification Pathway antibodies	Post-translational Protein Modification Pathway Diagram
Hypusine Biosynthesis Pathway antibodies	Hypusine Biosynthesis Pathway Diagram
Hypusine Biosynthesis Pathway antibodies	Hypusine Biosynthesis Pathway Diagram

Diseases associated with anti-DOHH antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>18 publications with DOHH and Neoplasms
Necrosis Antibodies	>5 publications with DOHH and Necrosis
Hyperplasia Antibodies	>1 publications with DOHH and Hyperplasia
Carcinoma, Hepatocellular Antibodies	>1 publications with DOHH and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-DOHH antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>9 publications with DOHH and Blood