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ZO1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS645406

ZO1 encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ZO1, Polyclonal Antibody
Full Product Name
ZO1, CT (TJP1, ZO1, Tight junction protein ZO-1, Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1)
Product Synonym Names
Anti -ZO1, CT (TJP1, ZO1, Tight junction protein ZO-1, Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Purified
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
ZO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1578~1607 amino acids from the C-terminal region of human ZO1.

Product Categories/Family for anti-ZO1 antibody

Antibodies; Abs to Connexin; Junction Proteins
Applications Tested/Suitable for anti-ZO1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-ZO1 antibody
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry
Dilution: ELISA: 1:1,000
Western Blot: 1:50-100
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-ZO1 antibody

Western blot analysis of ZO1 Antibody (C-term) in K562 cell line lysates (35ug/lane). ZO1 (arrow) was detected using the purified Pab.
Rabbit Anti-ZO1 Polyclonal Antibody – Cat# MBS645406

Immunohistochemistry (IHC) of anti-ZO1 antibody

ZO1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ZO1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-ZO1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS645406

Flow Cytometry (FC/FACS) of anti-ZO1 antibody

ZO1 Antibody (C-term) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-ZO1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS645406

Other Notes

Small volumes of anti-ZO1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ZO1 purchase

MBS645406 is an antibody targeting ZO1. Also known as ZO1, CT (TJP1, ZO1, Tight junction protein ZO-1, Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1). Reactive in Human. Host: Rabbit.
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