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GAA Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS645420

This gene encodes acid alpha-glucosidase, which is essential for the degradation of glycogen to glucose in lysosomes. Different forms of acid alpha-glucosidase are obtained by proteolytic processing. Defects in this gene are the cause of glycogen storage… More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GAA, Polyclonal Antibody
Full Product Name
GAA, NT (GAA, Lysosomal alpha-glucosidase, Acid maltase, Aglucosidase alfa, 76kD lysosomal alpha-glucosidase, 70kD lysosomal alpha-glucosidase)
Product Synonym Names
Anti -GAA, NT (GAA, Lysosomal alpha-glucosidase, Acid maltase, Aglucosidase alfa, 76kD lysosomal alpha-glucosidase, 70kD lysosomal alpha-glucosidase)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
GAA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 173-203 amino acids from the N-terminal region of human GAA.
Applications Tested/Suitable for anti-GAA antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-GAA antibody
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50

Western Blot (WB) of anti-GAA antibody

Western Blot analysis of lysate from Jurkat cell line (20ug/lane) using MBS645420 (1:2000). An IgG H&L (HRP) goat anti-rabbit (1:10,000) used as the secondary antibody.
Rabbit Anti-GAA Polyclonal Antibody – Cat# MBS645420

Western Blot (WB) of anti-GAA antibody

Western Blot analysis of lysates from A549, MCF-7, SW620, PC-3 cell line (20ug/lane) (from left to right), using MBS645420 (1:100). An IgG H&L (HRP) goat anti-rabbit (1:10,000) was used as the secondary antibody.
Rabbit Anti-GAA Polyclonal Antibody, Western Blot (WB) – Cat# MBS645420

Western Blot (WB) of anti-GAA antibody

Western Blot analysis in HL-60 cell line lysates (35ug/lane) using MBS645420.This demonstrates that MBS645420 detected the GAA protein (arrow).
Rabbit Anti-GAA Polyclonal Antibody, Western Blot (WB) – Cat# MBS645420

Immunohistochemistry (IHC) of anti-GAA antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma using MBS645420 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use MBS645420 for immunohistochemistry.
Rabbit Anti-GAA Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS645420

Other Notes

Small volumes of anti-GAA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GAA purchase

MBS645420 is an antibody targeting GAA. Also known as GAA, NT (GAA, Lysosomal alpha-glucosidase, Acid maltase, Aglucosidase alfa, 76kD lysosomal alpha-glucosidase, 70kD lysosomal alpha-glucosidase). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GAA. It may not necessarily be applicable to this product.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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