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KLF4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS645556

Kr¸ppel-like factor 4 (KLF4) is a transcription factor involved in both proliferation and differentiation in the colon. It is down-regulated in both mouse and human colonic adenomas and has been implicated as a tumor suppressor in the gut, whereas in breast… More details

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Product Overview

Online Datasheet in PDF
Product Name
KLF4, Polyclonal Antibody
Full Product Name
KLF4, NT (C74) (KLF4, EZF, GKLF, Krueppel-like factor 4, Epithelial zinc finger protein EZF, Gut-enriched krueppel-like factor)
Product Gene Name
anti-KLF4 antibody
Product Synonym Names
Anti -KLF4, NT (C74) (KLF4, EZF, GKLF, Krueppel-like factor 4, Epithelial zinc finger protein EZF, Gut-enriched krueppel-like factor)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Purified
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Specificity
Human, mouse
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
54,671 Da
Chromosome Location
Chromosome: 9; NC_000009.11 (110247133..110252047, complement). Location: 9q31
OMIM
602253
3D Structure
ModBase 3D Structure for O43474
Immunogen
KLF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68~98 amino acids from the N-terminal region of human KLF4.

Product Categories/Family for anti-KLF4 antibody

Antibodies; Abs to Transcription Factors; Zinc (Ring) Finger
Applications Tested/Suitable for anti-KLF4 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-KLF4 antibody
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Dilution: ELISA: 1:1,000
Western blot1:50-100
Immunohistochemistry: 1:10-50

Western Blot (WB) of anti-KLF4 antibody

Western blot analysis of KLF4 Antibody (N-term C74) in Jurkat,293 cell line lysates (35ug/lane).KLF4 (arrow) was detected using the purified Pab.
Rabbit Anti-KLF4 Polyclonal Antibody – Cat# MBS645556

Western Blot (WB) of anti-KLF4 antibody

Western blot analysis of KLF4 Antibody (N-term C74) in mouse stomach tissue lysates (35ug/lane). KLF4 (arrow) was detected using the purified Pab.
Rabbit Anti-KLF4 Polyclonal Antibody, Western Blot (WB) – Cat# MBS645556

Immunofluorescence (IF) of anti-KLF4 antibody

Fluorescent confocal image of HeLa cells stained with AP2725a KLF4 (N-term C74) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with AP2725a KLF4 (N-term C74) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (5.25 μM, 25 min). KLF4 immunoreactivity is localized to the nuclei in HeLa cells.
Rabbit Anti-KLF4 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS645556

Immunofluorescence (IF) of anti-KLF4 antibody

Fluorescent confocal image of SY5Y cells stained with AP2725a KLF4 (N-term C74) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with AP2725a KLF4 (N-term C74) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (5.25 μM, 25 min). KLF4 immunoreactivity is localized to the nuclei in SY5Y cells.
Rabbit Anti-KLF4 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS645556

Immunofluorescence (IF) of anti-KLF4 antibody

Fluorescent confocal image of SY5Y cells stained with AP2725a KLF4 (N-term C74) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with AP2725a KLF4 (N-term C74) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (5.25 μM, 25 min). KLF4 immunoreactivity is localized to the nuclei in SY5Y cells.
Rabbit Anti-KLF4 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS645556

Other Notes

Small volumes of anti-KLF4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KLF4 purchase

MBS645556 is an antibody targeting KLF4. Also known as KLF4, NT (C74) (KLF4, EZF, GKLF, Krueppel-like factor 4, Epithelial zinc finger protein EZF, Gut-enriched krueppel-like factor). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for KLF4. It may not necessarily be applicable to this product.

NCBI GI #
194248077
NCBI GeneID
9314
NCBI Accession #
NP_004226.3
UniProt Primary Accession #
O43474
UniProt Secondary Accession #
P78338; Q5T3J8; Q5T3J9; Q8N717; Q9UNP3; B2R8S4; B3KT79; L0R3I6; L0R4N5
UniProt Related Accession #
O43474
GenBank Nucleotide #
NM_004235.4
NCBI Official Full Name
Krueppel-like factor 4
NCBI Official Synonym Full Names
Kruppel-like factor 4 (gut)
NCBI Official Symbol
KLF4
NCBI Official Synonym Symbols
EZF; GKLF
UniProt Protein Name
Krueppel-like factor 4
UniProt Synonym Protein Names
Epithelial zinc finger protein EZF; Gut-enriched krueppel-like factor
Protein Family
Krueppel-like factor
UniProt Gene Name
KLF4
UniProt Synonym Gene Names
EZF; GKLF
UniProt Entry Name
KLF4_HUMAN
NCBI Protein Information
Krueppel-like factor 4; gut-enriched krueppel-like factor; epithelial zinc finger protein EZF; endothelial Kruppel-like zinc finger protein

UniProt Comments for KLF4

Function: Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription. Ref.10 Ref.15

Subunit structure: Interacts with POU5F1/OCT4 and SOX2

By similarity. Interacts with MUC1 (via the C-terminal domain). Interacts with MEIS2 isoform 4 and PBX1 isoform PBX1a. Ref.10 Ref.17

Subcellular location: Nucleus.

Domain: the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. Ref.12

Biotechnological use: POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 are the four Yamanaka factors. When combined, these factors are sufficient to reprogram differentiated cells to an embryonic-like state designated iPS (induced pluripotent stem) cells. iPS cells exhibit the morphology and growth properties of ES cells and express ES cell marker genes. Ref.11

Sequence similarities: Belongs to the krueppel C2H2-type zinc-finger protein family.Contains 3 C2H2-type zinc fingers.

Sequence caution: The sequence AAB48399.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAC03462.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAD42165.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAH29923.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAH30811.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence ABG25917.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAG36271.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence EAW59020.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence EAW59021.1 differs from that shown. Reason: Erroneous gene model prediction.
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Research Articles on KLF4

1. The findings of this study suggested an essential contribution of combined KLF4 K409Q and TRAF7 mutations in the genesis of secretory meningioma and demonstrate a role for TRAF7 alterations in other non-NF2 meningiomas.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-KLF4 antibody

 Reference Product  PubMed Publications
 SP1 antibody  >35 publications with KLF4 and SP1
 CTNNB1 antibody  >29 publications with KLF4 and CTNNB1
 TGFB1 antibody  >12 publications with KLF4 and TGFB1
 TP53 antibody  >11 publications with KLF4 and TP53
 HDAC2 antibody  >9 publications with KLF4 and HDAC2
 RELA antibody  >3 publications with KLF4 and RELA
 EP300 antibody  >2 publications with KLF4 and EP300
 KAT5 antibody  >1 publications with KLF4 and KAT5
 CREBBP antibody  >1 publications with KLF4 and CREBBP

Pathways associated with anti-KLF4 antibody

 Products by Pathway  Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Peptide Hormone Metabolism Pathway antibodies  Peptide Hormone Metabolism Pathway Diagram
 Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway antibodies  Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway Diagram
 SRF And MiRs In Smooth Muscle Differentiation And Proliferation Pathway antibodies  SRF And MiRs In Smooth Muscle Differentiation And Proliferation Pathway Diagram
 Synthesis, Secretion, And Deacylation Of Ghrelin Pathway antibodies  Synthesis, Secretion, And Deacylation Of Ghrelin Pathway Diagram
 Transcriptional Regulation Of White Adipocyte Differentiation Pathway antibodies  Transcriptional Regulation Of White Adipocyte Differentiation Pathway Diagram

Diseases associated with anti-KLF4 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >246 publications with KLF4 and Neoplasms
 Cardiovascular Diseases Antibodies  >51 publications with KLF4 and Cardiovascular Diseases
 Nervous System Diseases Antibodies  >44 publications with KLF4 and Nervous System Diseases
 Inflammation Antibodies  >41 publications with KLF4 and Inflammation
 Disease Models, Animal Antibodies  >40 publications with KLF4 and Disease Models, Animal
 Breast Neoplasms Antibodies  >30 publications with KLF4 and Breast Neoplasms
 Brain Diseases Antibodies  >28 publications with KLF4 and Brain Diseases
 Adenocarcinoma Antibodies  >26 publications with KLF4 and Adenocarcinoma
 Atherosclerosis Antibodies  >25 publications with KLF4 and Atherosclerosis
 Heart Diseases Antibodies  >24 publications with KLF4 and Heart Diseases

Organs/Tissues associated with anti-KLF4 antibody

 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >164 publications with KLF4 and Embryonic Tissue
 Skin Antibodies  >104 publications with KLF4 and Skin
 Vascular Antibodies  >85 publications with KLF4 and Vascular
 Heart Antibodies  >58 publications with KLF4 and Heart
 Brain Antibodies  >51 publications with KLF4 and Brain
 Liver Antibodies  >44 publications with KLF4 and Liver
 Intestine Antibodies  >42 publications with KLF4 and Intestine
 Eye Antibodies  >36 publications with KLF4 and Eye
 Lung Antibodies  >31 publications with KLF4 and Lung
 Kidney Antibodies  >23 publications with KLF4 and Kidney
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