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HECTD1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS645574

Probable E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. May be required for development of the head mesenchyme and neural… More details

$880 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HECTD1, Monoclonal Antibody
Full Product Name
HECTD1 (E3 Ubiquitin-protein Ligase HECTD1, E3 Ligase for Inhibin Receptor, EULIR, HECT Domain-containing Protein 1, KIAA1131)
Product Synonym Names
Anti -HECTD1 (E3 Ubiquitin-protein Ligase HECTD1, E3 Ligase for Inhibin Receptor, EULIR, HECT Domain-containing Protein 1, KIAA1131)
Immunogen Sequence
DVDPDTLLEW LQMGQGDERD MQLIALEQLC MLLLMSDNVD RCFETCPPRT FLPALCKIFL DESAPDNVLE VTARAITYYL DVSAECTRRI VGVDGAIKAL CNRLVVVE*
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
1E10
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human HECTD1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Partial recombinant corresponding to aa3-111 from human HECTD1 (NP_056197) with GST tag. MW of the GST tag alone is 26kD.
Applications Tested/Suitable for anti-HECTD1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-HECTD1 antibody
Suitable for use in ELISA and Western Blot.

Western Blot (WB) of anti-HECTD1 antibody

Western Blot detection against Immunogen (37.99kD).
Mouse Anti-HECTD1 Monoclonal Antibody – Cat# MBS645574

Testing Data of anti-HECTD1 antibody

Detection limit for recombinant GST tagged HECTD1 is ~0.3ng/ml as a capture antibody.
Mouse Anti-HECTD1 Monoclonal Antibody, Testing Data – Cat# MBS645574

Other Notes

Small volumes of anti-HECTD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HECTD1 purchase

MBS645574 is an antibody targeting HECTD1. Also known as HECTD1 (E3 Ubiquitin-protein Ligase HECTD1, E3 Ligase for Inhibin Receptor, EULIR, HECT Domain-containing Protein 1, KIAA1131). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for HECTD1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
NCBI Official Full Name
HECTD1

UniProt Comments for HECTD1

HECTD1: a ubiquitin-protein ligase required for development of the head mesenchyme and neural tube closure. Accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.

Protein type: EC 6.3.2.-; Ubiquitin ligase; Ligase; EC 6.3.2.19; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 14q12

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; metal ion binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: protein ubiquitination during ubiquitin-dependent protein catabolic process; neural tube closure
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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