• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

HSD17B12 Antibody – Mouse Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS645578

The enzyme 17-beta hydroxysteroid dehydrogenase-12 (HSD17B12) uses NADPH to reduce 3-ketoacyl-CoA to 3-hydroxyacyl-CoA during the second step of fatty acid elongation. More details

$880 / 0.05 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HSD17B12, Polyclonal Antibody
Full Product Name
HSD17B12 (Estradiol 17-beta-dehydrogenase 12, 17-beta-hydroxysteroid Dehydrogenase 12, 3-ketoacyl-CoA Reductase)
Product Gene Name
anti-HSD17B12 antibody
Product Synonym Names
Anti -HSD17B12 (Estradiol 17-beta-dehydrogenase 12, 17-beta-hydroxysteroid Dehydrogenase 12, 3-ketoacyl-CoA Reductase)
Immunogen Sequence
MESALPAAGF LYWVGAGTVA YLALRISYSL FTALRVWGVG NEAGVGPGLG EWAVVTGSTD GIGKSYAEEL AKHGMKVVLI SRSKDKLDQV SSEISNYT
Clonality
Polyclonal
Isotype
IgG
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human HSD17B12.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
34,324 Da
Chromosome Location
Chromosome: 11; NC_000011.9 (43702108..43878169). Location: 11p11.2
OMIM
609574
3D Structure
ModBase 3D Structure for Q53GQ0
Immunogen
Full length human HSD17B12, aa1-98 (AAH12536.1).

Product Categories/Family for anti-HSD17B12 antibody

Antibodies; Abs to Enzymes; Dehydrogenase
Applications Tested/Suitable for anti-HSD17B12 antibody
Western Blot (WB)
Application Notes for anti-HSD17B12 antibody
Suitable for use in Western Blot.

Western Blot (WB) of anti-HSD17B12 antibody

Western Blot analysis of HSD17B12 expression in transfected 293T cell line by HSD17B12 polyclonal antibody. Lane 1: HSD17B12 transfected lysate (10.78kD). Lane 2: Non-transfected lysate.
Mouse Anti-HSD17B12 Polyclonal Antibody – Cat# MBS645578

Other Notes

Small volumes of anti-HSD17B12 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HSD17B12 purchase

MBS645578 is an antibody targeting HSD17B12. Also known as HSD17B12 (Estradiol 17-beta-dehydrogenase 12, 17-beta-hydroxysteroid Dehydrogenase 12, 3-ketoacyl-CoA Reductase). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for HSD17B12. It may not necessarily be applicable to this product.

NCBI GI #
15214803
NCBI GeneID
51144
NCBI Accession #
AAH12536.1
UniProt Primary Accession #
Q53GQ0
UniProt Secondary Accession #
Q96JU2; Q9Y6G8; A8K9B0; D3DR23
UniProt Related Accession #
Q53GQ0
NCBI Official Full Name
HSD17B12 protein
NCBI Official Synonym Full Names
hydroxysteroid (17-beta) dehydrogenase 12
NCBI Official Symbol
HSD17B12
NCBI Official Synonym Symbols
KAR; SDR12C1
UniProt Protein Name
Estradiol 17-beta-dehydrogenase 12
UniProt Synonym Protein Names
17-beta-hydroxysteroid dehydrogenase 12; 17-beta-HSD 12; 3-ketoacyl-CoA reductase
Protein Family
Very-long-chain 3-oxoacyl-CoA reductase
UniProt Gene Name
HSD17B12
UniProt Synonym Gene Names
17-beta-HSD 12; KAR
UniProt Entry Name
DHB12_HUMAN
NCBI Protein Information
estradiol 17-beta-dehydrogenase 12; 17-beta-HSD 12; 17beta-HSD type 12; 3-ketoacyl-CoA reductase; steroid dehydrogenase homolog; 17-beta-hydroxysteroid dehydrogenase 12; short chain dehydrogenase/reductase family 12C, member 1

NCBI Summary for HSD17B12

This gene encodes a very important 17beta-hydroxysteroid dehydrogenase (17beta-HSD) that converts estrone into estradiol in ovarian tissue. This enzyme is also involved in fatty acid elongation. [provided by RefSeq, Oct 2011]

UniProt Comments for HSD17B12

Function: Catalyzes the transformation of estrone (E1) into estradiol (E2), suggesting a central role in estrogen formation. Its strong expression in ovary and mammary gland suggest that it may constitute the major enzyme responsible for the conversion of E1 to E2 in women. Also has 3-ketoacyl-CoA reductase activity, reducing both long chain 3-ketoacyl-CoAs and long chain fatty acyl-CoAs, suggesting a role in long fatty acid elongation. Ref.7 Ref.9

Catalytic activity: 17-beta-estradiol + NAD(P)+ = estrone + NAD(P)H.

Pathway: Steroid biosynthesis; estrogen biosynthesis.Lipid metabolism; fatty acid biosynthesis.

Subunit structure: Interacts with ELOVL1 and LASS2. Ref.10

Subcellular location: Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.7.

Tissue specificity: Expressed in most tissues tested. Highly expressed in the ovary and mammary. Expressed in platelets. Ref.7 Ref.8 Ref.9

Domain: The di-lysine motif confers endoplasmic reticulum localization for type I membrane proteins

By similarity.

Sequence similarities: Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.

Biophysicochemical propertiesKinetic parameters:KM=3.5 µM for estrone Ref.9

Sequence caution: The sequence AK027882 differs from that shown. Reason: Frameshift at position 92.
Show more

Research Articles on HSD17B12

1. These evidences were suggestive of a 46,XY DSD due to 17betaHSD3 deficiency. An homozygous mutation (IVS3 -1 G>C or c.326-1G>C) of the 17betaHSD3 gene was discovered.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-HSD17B12 antibody

 Products by Pathway  Pathway Diagram
 Biosynthesis Of Unsaturated Fatty Acids Pathway antibodies  Biosynthesis Of Unsaturated Fatty Acids Pathway Diagram
 Biosynthesis Of Unsaturated Fatty Acids Pathway antibodies  Biosynthesis Of Unsaturated Fatty Acids Pathway Diagram
 Fatty Acyl-CoA Biosynthesis Pathway antibodies  Fatty Acyl-CoA Biosynthesis Pathway Diagram
 Fatty Acid Biosynthesis, Elongation, Endoplasmic Reticulum Pathway antibodies  Fatty Acid Biosynthesis, Elongation, Endoplasmic Reticulum Pathway Diagram
 Fatty Acid Biosynthesis, Elongation, Endoplasmic Reticulum Pathway antibodies  Fatty Acid Biosynthesis, Elongation, Endoplasmic Reticulum Pathway Diagram
 Fatty Acid Elongation Pathway antibodies  Fatty Acid Elongation Pathway Diagram
 Fatty Acid Elongation Pathway antibodies  Fatty Acid Elongation Pathway Diagram
 Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway antibodies  Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram

Diseases associated with anti-HSD17B12 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >11 publications with HSD17B12 and Neoplasms
 Breast Neoplasms Antibodies  >4 publications with HSD17B12 and Breast Neoplasms
 Neoplasms, Experimental Antibodies  >3 publications with HSD17B12 and Neoplasms, Experimental
 Prostatic Neoplasms Antibodies  >3 publications with HSD17B12 and Prostatic Neoplasms
 Inflammation Antibodies  >2 publications with HSD17B12 and Inflammation
 Lung Diseases Antibodies  >1 publications with HSD17B12 and Lung Diseases
 Lung Neoplasms Antibodies  >1 publications with HSD17B12 and Lung Neoplasms

Organs/Tissues associated with anti-HSD17B12 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >7 publications with HSD17B12 and Blood
 Prostate Antibodies  >3 publications with HSD17B12 and Prostate
 Lymph Antibodies  >1 publications with HSD17B12 and Lymph
 Mammary Gland Antibodies  >1 publications with HSD17B12 and Mammary Gland
 Vascular Antibodies  >1 publications with HSD17B12 and Vascular
 Embryonic Tissue Antibodies  >1 publications with HSD17B12 and Embryonic Tissue
 Lung Antibodies  >1 publications with HSD17B12 and Lung
 Uncharacterized Tissue Antibodies  >1 publications with HSD17B12 and Uncharacterized Tissue
 Testis Antibodies  >1 publications with HSD17B12 and Testis
 Lymph Node Antibodies  >1 publications with HSD17B12 and Lymph Node
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000