PLA2G16 Antibody – Goat Polyclonal

Function: Exhibits PLA1/2 activity, catalyzing the calcium-independent hydrolysis of acyl groups in various phosphatidylcholines (PC) and phosphatidylethanolamine (PE). For most substrates, PLA1 activity is much higher than PLA2 activity. Specifically catalyzes the release of fatty acids from phospholipids in adipose tissue

By similarity. N- and O-acylation activity is hardly detectable. Might decrease protein phosphatase 2A (PP2A) activity. Ref.4 Ref.10

Catalytic activity: Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate. Ref.12 Ref.13Phosphatidylcholine + H2O = 2-acylglycerophosphocholine + a carboxylate. Ref.12 Ref.13

Subunit structure: Interacts with PPP2R1A; this interaction might decrease PP2A activity. Ref.10

Subcellular location: Cytoplasm. Cytoplasm › perinuclear region

By similarity. Membrane; Single-pass membrane protein Ref.10 Ref.12.

Tissue specificity: Widely expressed. low expression, if any, in hematopoietic cells and thymus. In testis, confined to round spermatids. Expressed in normal ovarian epithelial cells. Down-regulated in some ovarian carcinomas and testicular germ cell tumors. Ref.1 Ref.3 Ref.4 Ref.9

Induction: By IFNG and IRF1. Ref.9

Sequence similarities: Belongs to the H-rev107 family.

Biophysicochemical propertiesKinetic parameters:KM=300 µM for dipalmitoyl-PC Ref.4Vmax=2.57 µmol/min/mg enzyme with dipalmitoyl-PC as substrateVmax=267 nmol/min/mg enzyme with dipalmitoyl-PE as substratepH dependence:Optimum pH is 9.