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T286 (NLK) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS645645

MBS645645 is an antibody targeting NLK-T286. Also known as NLK-T286, ID (NLK, LAK1, Serine/threonine-protein kinase NLK, Nemo-like kinase, Protein LAK1). Reactive in Human. Host: Rabbit. More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NLK-T286, Polyclonal Antibody
Full Product Name
NLK-T286, ID (NLK, LAK1, Serine/threonine-protein kinase NLK, Nemo-like kinase, Protein LAK1)
Product Synonym Names
Anti -NLK-T286, ID (NLK, LAK1, Serine/threonine-protein kinase NLK, Nemo-like kinase, Protein LAK1)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
NLK-T286 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 268-298 amino acids from the Central region of human PMAT-Y198.

Product Categories/Family for anti-NLK-T286 antibody

Antibodies; Abs to Protein Kinases
Applications Tested/Suitable for anti-NLK-T286 antibody
ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-NLK-T286 antibody
Suitable for use in Western Blot, Immunofluorescence, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50
Immunofluorescence: 1:10-50

Western Blot (WB) of anti-NLK-T286 antibody

NLK-T286 western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the NLK antibody detected the NLK protein (arrow).
Rabbit Anti-NLK Polyclonal Antibody – Cat# MBS645645

Flow Cytometry (FC/FACS) of anti-NLK-T286 antibody

NLK-T286 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-NLK Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS645645

Immunofluorescence (IF) of anti-NLK-T286 antibody

Confocal immunofluorescent analysis of NLK-T286 Antibody (Center) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-NLK Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS645645

Other Notes

Small volumes of anti-NLK-T286 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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