Mouse Anti-RFXAP Polyclonal Antibody – Cat# MBS645659

Western Blot analysis of RFXAP expression in transfected 293T cell line by RFXAP polyclonal antibody. Lane 1: RFXAP transfected lysate (29.92kD). Lane 2: Non-transfected lysate.

RFXAP Antibody – Mouse Polyclonal

Name: RFXAP Antibody; Polyclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS645659
Price: 880 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS645659

Major histocompatibility (MHC) class II molecules are transmembrane proteins that have a central role in development and control of the immune system. The protein encoded by this gene, along with regulatory factor X-associated ankyrin-containing protein and… More details

$880 / 0.05 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

RFXAP, Polyclonal Antibody

Full Product Name

RFXAP (Regulatory Factor X Associated Protein, Regulatory Factor X-associated Protein, RFX-associated Protein, RFX DNA-binding Complex 36kD Subunit)

Product Gene Name

anti-RFXAP antibody

Product Synonym Names

Anti -RFXAP (Regulatory Factor X Associated Protein, Regulatory Factor X-associated Protein, RFX-associated Protein, RFX DNA-binding Complex 36kD Subunit)

Immunogen Sequence

MEAQSVAEGA GPGAASGVPH PAALAPAAAP TLAPASVAAA ASQFTLLVMQ PCAGQDEAAA PGGSVGAGKP VRYLCEGAGD GEEEAGEDEA DLLDTSDPPG GGESAASLED LEDEETHSGG EGSSGGARRR GSGGGSMSKT CTYEGCSETT SQVAKQRKPW MCKKHRNKMY KDKYKKKKSD QALNCGGTAS TGSAGNVKLE ESADNILSIV KQRTGSFGDR PARPTLLEQV LNQKRLSLLR SPEVVQFLQK QQQLLNQQVL EQRQQQFPGT SM

Clonality

Polyclonal

Isotype

IgG

Host

Mouse

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2.

Purity/Purification

Affinity Purified
Purified by Protein A affinity chromatography.

Specificity

Recognizes human RFXAP.

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

28,232 Da

Chromosome Location

Chromosome: 13; NC_000013.10 (37393339..37403740). Location: 13q14

OMIM

209920

3D Structure

ModBase 3D Structure for O00287

Immunogen

Full length human RFXAP, aa1-272 (AAH26088.1).

Product Categories/Family for anti-RFXAP antibody

Antibodies; Abs to Transcription Factors

Applications Tested/Suitable for anti-RFXAP antibody

Western Blot (WB)

Application Notes for anti-RFXAP antibody

Suitable for use in Western Blot.

Other Notes

Small volumes of anti-RFXAP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RFXAP purchase

MBS645659 is an antibody targeting RFXAP. Also known as RFXAP (Regulatory Factor X Associated Protein, Regulatory Factor X-associated Protein, RFX-associated Protein, RFX DNA-binding Complex 36kD Subunit). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for RFXAP. It may not necessarily be applicable to this product.

NCBI GI #

4506501

NCBI GeneID

5994

NCBI Accession #

NP_000529.1

UniProt Primary Accession #

O00287

UniProt Secondary Accession #

Q5VZM6; Q8TC40; B2R9T8

UniProt Related Accession #

O00287

GenBank Nucleotide #

NM_000538.3

NCBI Official Full Name

regulatory factor X-associated protein

NCBI Official Synonym Full Names

regulatory factor X-associated protein

NCBI Official Symbol

RFXAP

UniProt Protein Name

Regulatory factor X-associated protein

UniProt Synonym Protein Names

RFX DNA-binding complex 36 kDa subunit

Protein Family

Regulatory factor X-associated protein

UniProt Gene Name

RFXAP

UniProt Synonym Gene Names

RFX-associated protein

UniProt Entry Name

RFXAP_HUMAN

NCBI Protein Information

regulatory factor X-associated protein; RFX-associated protein; RFX DNA-binding complex 36 kDa subunit

UniProt Comments for RFXAP

Function: Part of the RFX complex that binds to the X-box of MHC II promoters.

Subunit structure: The RFX heterotetrameric complex consists of 2 molecules of RFX5 and one each of RFXAP and RFX-B/RFXANK; with each subunit representing a separate complementation group. RFX forms cooperative DNA binding complexes with X2BP and CBF/NF-Y. RFX associates with CIITA to form an active transcriptional complex. Ref.8

Subcellular location: Nucleus.

Tissue specificity: Ubiquitous.

Domain: The C-terminal domain is necessary for the RFX complex formation. Ref.8

Post-translational modification: Phosphorylated.

Involvement in disease: Bare lymphocyte syndrome 2 (BLS2) [MIM:209920]: A severe combined immunodeficiency disease with early onset. It is characterized by a profound defect in constitutive and interferon-gamma induced MHC II expression, absence of cellular and humoral T-cell response to antigen challenge, hypogammaglobulinemia and impaired antibody production. The consequence include extreme susceptibility to viral, bacterial and fungal infections.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.1 Ref.6

Research Articles on RFXAP

1. RFXAP(C) consists of two alpha-helices that form a V-shaped structure that packs within the RFX5(N)(2) staple.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RFXAP antibody

Reference Product	PubMed Publications
RFX5 antibody	>35 publications with RFXAP and RFX5
CIITA antibody	>28 publications with RFXAP and CIITA
RFXANK antibody	>27 publications with RFXAP and RFXANK
SMARCA4 antibody	>1 publications with RFXAP and SMARCA4

Pathways associated with anti-RFXAP antibody

Products by Pathway	Pathway Diagram
Antigen Processing And Presentation Pathway antibodies	Antigen Processing And Presentation Pathway Diagram
Antigen Processing And Presentation Pathway antibodies	Antigen Processing And Presentation Pathway Diagram
Primary Immunodeficiency Pathway antibodies	Primary Immunodeficiency Pathway Diagram
Primary Immunodeficiency Pathway antibodies	Primary Immunodeficiency Pathway Diagram
Tuberculosis Pathway antibodies	Tuberculosis Pathway Diagram
Tuberculosis Pathway antibodies	Tuberculosis Pathway Diagram

Diseases associated with anti-RFXAP antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>2 publications with RFXAP and Neoplasms
Neoplasms, Experimental Antibodies	>1 publications with RFXAP and Neoplasms, Experimental
Inflammation Antibodies	>1 publications with RFXAP and Inflammation
Bare lymphocyte syndrome 2 Antibodies	>1 publications with RFXAP and Bare lymphocyte syndrome 2

Organs/Tissues associated with anti-RFXAP antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>14 publications with RFXAP and Blood
Embryonic Tissue Antibodies	>1 publications with RFXAP and Embryonic Tissue
Placenta Antibodies	>1 publications with RFXAP and Placenta