LIME1 Antibody – Rabbit Polyclonal

Function: Involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and TCR (T-cell antigen receptor)-mediated T-cell signaling in T-cells. In absence of TCR signaling, may be involved in CD4-mediated inhibition of T-cell activation. Couples activation of these receptors and their associated kinases with distal intracellular events such as calcium mobilization or MAPK activation through the recruitment of PLCG2, GRB2, GRAP2, and other signaling molecules. Ref.1 Ref.3

Subunit structure: When phosphorylated in response to TCR stimulation and/or CD4 costimulation, interacts with LCK, CSK, FYN, PTPN11/SHP2, GRB2, PIK3R1 and GRAP2

By similarity. When phosphorylated in response to BCR activation, interacts with LYN, PIK3R1, PLCG2 and GRB2. Ref.1 Ref.3

Subcellular location: Cell membrane; Single-pass type III membrane protein. Note: Present in lipid rafts. Recruited to the immunological synapse upon conjugation of T-cell with antigen-presenting cell. Ref.1

Tissue specificity: Expressed in spleen and lung. Present in primary B-cells and peripheral T-cells (at protein level). Ref.1 Ref.3

Induction: Up-regulated in T-cells following TCR engagement. Ref.1

Post-translational modification: Palmitoylation of Cys-28 and Cys-31 is required for raft targeting. Ref.1Phosphorylated on tyrosines upon TCR activation and/or CD4 coreceptor stimulation, or upon BCR stimulation; which leads to the recruitment of SH2-containing proteins. Ref.1 Ref.3