PITPNM1 Antibody – Rabbit Polyclonal

Function: Regulates RHOA activity, and plays a role in cytoskeleton remodeling. Necessary for normal completion of cytokinesis. Plays a role in maintaining normal diacylglycerol levels in the Golgi apparatus. Binds phosphatidyl inositol phosphates (in vitro). May catalyze the transfer of phosphatidylinositol and phosphatidylcholine between membranes

By similarity. Necessary for maintaining the normal structure of the endoplasmic reticulum and the Golgi apparatus. Required for protein export from the endoplasmic reticulum and the Golgi. Binds calcium ions. Ref.2 Ref.8 Ref.10 Ref.11

Subunit structure: Interacts with PIK4CA

By similarity. Interacts with PTK2B via its C-terminus. Interacts with RHOA. Has higher affinity for the inactive, GDP-bound form of RHOA. The CDK1-phosphorylated form interacts with PLK1. Interacts with VAPB. Ref.2 Ref.8 Ref.9 Ref.10

Subcellular location: Cytoplasm. Golgi apparatus › Golgi stack membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Lipid droplet. Cleavage furrow. Midbody. Note: Peripheral membrane protein associated with Golgi stacks in interphase cells. A minor proportion is associated with the endoplasmic reticulum. Associated with lipid droplets. Dissociates from the Golgi early on in mitosis and localizes to the cleavage furrow and midbody during cytokinesis. Ref.7 Ref.9

Tissue specificity: Ubiquitous. Ref.2

Post-translational modification: Phosphorylated on multiple sites by CDK1 at the onset of mitosis. Phosphorylation facilitates dissociation from the Golgi complex and is required for interaction with PLK1. Ref.2 Ref.7 Ref.9Phosphorylated on threonine residues upon treatment with oleic acid. Ref.2 Ref.7 Ref.9Phosphorylated on tyrosine residues by PTK2B. Ref.2 Ref.7 Ref.9

Sequence similarities: Belongs to the PtdIns transfer protein family. PI transfer class IIA subfamily.Contains 1 DDHD domain.