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BFAR Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS645887

BFAR is a apoptosis regulator. Has anti-apoptotic activity, both for apoptosis triggered via death-receptors and via mitochondrial factors. More details

$880 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
BFAR, Monoclonal Antibody
Full Product Name
BFAR (Bifunctional Apoptosis Regulator, RING Finger Protein 47, BAR, RNF47)
Product Gene Name
Product Synonym Names
Anti -BFAR (Bifunctional Apoptosis Regulator, RING Finger Protein 47, BAR, RNF47)
Immunogen Sequence
LRFEDIQQNN DIVQSLAAFQ KYGNDQIPLA PNTGRANQQM GGGFFSGVLT ALTGVAVVLL VYHWSSRESE HDLLVHKAVA KWTAEEVVLW LEQLGPWASL
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
1C6
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human BFAR.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
52,738 Da
Chromosome Location
Chromosome: 16; NC_000016.9 (14726668..14763093). Location: 16p13.12
3D Structure
ModBase 3D Structure for Q9NZS9
Immunogen
Partial recombinant corresponding to aa101-200 from human BFAR (AAH03054) with GST tag. MW of the GST tag alone is 26kD.

Product Categories/Family for anti-BFAR antibody

Applications Tested/Suitable for anti-BFAR antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-BFAR antibody
Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml

Western Blot (WB) of anti-BFAR antibody

Western Blot detection against Immunogen (37kD).
Mouse Anti-BFAR Monoclonal Antibody – Cat# MBS645887

Western Blot (WB) of anti-BFAR antibody

Western Blot analysis of BFAR expression in transfected 293T cell line by BFAR monoclonal antibody.|Lane 1: BFAR transfected lysate (52.7kD).|Lane 2: Non-transfected lysate.
Mouse Anti-BFAR Monoclonal Antibody, Western Blot (WB) – Cat# MBS645887

Immunohistochemistry (IHC) of anti-BFAR antibody

Immunoperoxidase of monoclonal antibody to BFAR on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml].
Mouse Anti-BFAR Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS645887

Western Blot (WB) of anti-BFAR antibody

Western blot analysis of BFAR over-expressed 293 cell line, cotransfected with BFAR Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with BFAR monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.
Mouse Anti-BFAR Monoclonal Antibody, Western Blot (WB) – Cat# MBS645887

Other Notes

Small volumes of anti-BFAR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BFAR purchase

MBS645887 is an antibody targeting BFAR. Also known as BFAR (Bifunctional Apoptosis Regulator, RING Finger Protein 47, BAR, RNF47). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for BFAR. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
bifunctional apoptosis regulator
NCBI Official Synonym Full Names
bifunctional apoptosis regulator
NCBI Official Symbol
BFAR
NCBI Official Synonym Symbols
BAR; RNF47
UniProt Protein Name
Bifunctional apoptosis regulator
UniProt Synonym Protein Names
RING finger protein 47
UniProt Gene Name
BFAR
UniProt Synonym Gene Names
BAR; RNF47
UniProt Entry Name
BFAR_HUMAN
NCBI Protein Information
bifunctional apoptosis regulator; RING finger protein 47; bifunctional apoptosis inhibitor

UniProt Comments for BFAR

Function: Apoptosis regulator. Has anti-apoptotic activity, both for apoptosis triggered via death-receptors and via mitochondrial factors. Ref.5

Subunit structure: Interacts with CASP8, BCL2 and BCL2L1 through SAM domain and also with HIP1, IFT57, ESRRBL1 and BCAP31. Ref.1 Ref.5

Subcellular location: Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.1 Ref.5.

Tissue specificity: Expressed highly in brain, moderately in small intestine, weakly in testes and only faintly in liver and skeletal muscle. Not expressed in heart, kidney, lung and spleen. Ref.1 Ref.5

Sequence similarities: Contains 1 RING-type zinc finger.Contains 1 SAM (sterile alpha motif) domain.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-BFAR antibody

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