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PAPD7 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS645899

The protein encoded by this gene is a DNA polymerase that is likely involved in DNA repair. In addition, the encoded protein may be required for sister chromatid adhesion. Alternatively spliced transcript variants that encode different isoforms have been… More details

$880 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PAPD7, Monoclonal Antibody
Full Product Name
PAPD7 (POLS, DNA Polymerase sigma, DNA Polymerase kappa, LAK-1, PAP-associated Domain-containing Protein 7, Terminal Uridylyltransferase 5, TUTase 5, Topoisomerase-related Function Protein 4-1, TRF4-1, TRF4, TUTASE5)
Product Synonym Names
Anti -PAPD7 (POLS, DNA Polymerase sigma, DNA Polymerase kappa, LAK-1, PAP-associated Domain-containing Protein 7, Terminal Uridylyltransferase 5, TUTase 5, Topoisomerase-related Function Protein 4-1, TRF4-1, TRF4, TUTASE5)
Immunogen Sequence
SPCPEEAAMR REVVKRIETV VKDLWPTADV QIFGSFSTGL YLPTSDIDLV VFGKWERPPL QLLEQALRKH NVAEPCSIKV LDKATVPIIK LTDQETEVKV DISFNMETG
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
2F8
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human POLS.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Partial recombinant corresponding to aa2-111 from human POLS (NP_008930) with GST tag. MW of the GST tag alone is 26kD.
Applications Tested/Suitable for anti-PAPD7 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-PAPD7 antibody
Suitable for use in ELISA and Western Blot.

Western Blot (WB) of anti-PAPD7 antibody

Western Blot detection against Immunogen (38.1kD).
Mouse Anti-PAPD7 Monoclonal Antibody – Cat# MBS645899

Testing Data of anti-PAPD7 antibody

Detection limit for recombinant GST tagged POLS is ~0.3ng/ml as a capture antibody.
Mouse Anti-PAPD7 Monoclonal Antibody, Testing Data – Cat# MBS645899

Western Blot (WB) of anti-PAPD7 antibody

Western blot analysis of POLS over-expressed 293 cell line, cotransfected with POLS Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with POLS monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.
Mouse Anti-PAPD7 Monoclonal Antibody, Western Blot (WB) – Cat# MBS645899

Other Notes

Small volumes of anti-PAPD7 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PAPD7 purchase

MBS645899 is an antibody targeting PAPD7. Also known as PAPD7 (POLS, DNA Polymerase sigma, DNA Polymerase kappa, LAK-1, PAP-associated Domain-containing Protein 7, Terminal Uridylyltransferase 5, TUTase 5, Topoisomerase-related Function Protein 4-1, TRF4-1, TRF4, TUTASE5). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PAPD7. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
NCBI Official Full Name
PAPD7

UniProt Comments for PAPD7

POLS: DNA polymerase, probably involved in DNA repair. May play a role in sister chromatid cohesion. Does not play a role in replication-dependent histone mRNA degradation. Belongs to the DNA polymerase type-B-like family.

Protein type: DNA repair, damage; EC 2.7.7.19; DNA replication; Transferase

Chromosomal Location of Human Ortholog: 5p15

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: polynucleotide adenylyltransferase activity; DNA binding; SMC protein binding; metal ion binding; DNA-directed DNA polymerase activity

Biological Process: response to drug; mitotic chromosome condensation; cell division; double-strand break repair; sister chromatid cohesion
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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