FBXL5 Antibody – Rabbit Polyclonal

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains several tandem leucine-rich repeats. Alternatively spliced transcript variants have been described for this locus. [provided by RefSeq, Aug 2010]

Function: Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in iron homeostasis by promoting the ubiquitination and subsequent degradation of IREB2/IRP2. Upon high iron and oxygen level, it specifically recognizes and binds IREB2/IRP2, promoting its ubiquitination and degradation by the proteasome. Promotes ubiquitination and subsequent degradation of DCTN1/p150-glued. Ref.11 Ref.12 Ref.13

Pathway: Protein modification; protein ubiquitination.

Subunit structure: Part of a SCF (SKP1-cullin-F-box) protein ligase complex. Interacts with ACO1/IRP1, IREB2/IRP2; the interaction depends on the 4Fe-4S cluster. Interacts with DCTN1/p150-glued. Ref.11 Ref.12 Ref.13

Subcellular location: Cytoplasm › perinuclear region Ref.11.

Domain: The hemerythrin-like region acts as an oxygen and iron sensor by binding oxygen through a diiron metal-center. In absence of oxygen and iron, the protein is ubiquitinated and degraded. Ref.12 Ref.13

Post-translational modification: Polybiquitinated upon iron and oxygen depletion, leading to its degradation by the proteasome. Ubiquitination is regulated by the hemerythrin-like region that acts as an oxygen and iron sensor.

Sequence similarities: Contains 1 F-box domain.Contains 7 LRR (leucine-rich) repeats.

Sequence caution: The sequence AAF66616.1 differs from that shown. Reason: Erroneous initiation. The sequence AAF67489.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH30656.1 differs from that shown. Reason: Erroneous initiation. The sequence EAW92737.1 differs from that shown. Reason: Erroneous gene model prediction.