SULT2B1 Antibody – Mouse Monoclonal

Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene sulfates dehydroepiandrosterone but not 4-nitrophenol, a typical substrate for the phenol and estrogen sulfotransferase subfamilies. Two alternatively spliced variants that encode different isoforms have been described. [provided by RefSeq, Jul 2008]

Function: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs and xenobiotic compounds. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. Sulfates hydroxysteroids like DHEA. Isoform 1 preferentially sulfonates cholesterol, and isoform 2 avidly sulfonates pregnenolone but not cholesterol. Ref.1 Ref.4

Catalytic activity: 3'-phosphoadenylyl sulfate + an alcohol = adenosine 3',5'-bisphosphate + an alkyl sulfate.

Subcellular location: Cytoplasm. Microsome. Nucleus. Note: Phosphorylation of Ser-348 is required for translocation to the nucleus. Ref.5 Ref.6

Tissue specificity: Expressed highly in placenta, prostate and trachea and lower expression in the small intestine and lung. Ref.1

Sequence similarities: Belongs to the sulfotransferase 1 family.

Biophysicochemical propertiesKinetic parameters:KM=10.9 µM for DHEA (at 37 degrees Celsius, in the presence of 1mM MgCl2) Ref.5 Ref.6KM=3.8 µM for DHEA (at 37 degrees Celsius, in the presence of 10mM MgCl2)KM=11.8 µM for pregnenolone (at 37 degrees Celsius, in the presence of 1mM MgCl2)KM=0.6 µM for PAPS (at 37 degrees Celsius, in the presence of 1mM MgCl2)Vmax=1752 pmol/min/mg enzyme toward DHEA (at 37 degrees Celsius, in the presence of 10 mM MgCl2)Temperature dependence:Optimum temperature is 37 degrees Celsius. Retains 70% and 20% of activity when incubated at 42 degrees Celsius for 45 and 120 minutes, respectively. Activity is lost after 200 minutes incubation at 42 degrees Celsius.