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RPL11 (rplK) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS645998

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
RPL11 (rplK), Polyclonal Antibody
Full Product Name
RPL11, NT (RPL11, 60S ribosomal protein L11, CLL-associated antigen KW-12)
Product Gene Name
Product Synonym Names
Anti -RPL11, NT (RPL11, 60S ribosomal protein L11, CLL-associated antigen KW-12)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
14,934 Da
Chromosome Location
NC_002663.1 (1965655..1966083, complement)
3D Structure
ModBase 3D Structure for Q9CK85
Immunogen
RPL11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human RPL11.
Applications Tested/Suitable for anti-rplK antibody
ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-rplK antibody
Suitable for use in Western Blot, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-rplK antibody

Western Blot analysis in Jurkat cell line lysates (35ug/lane) using MBS645998. This demonstrates that MBS645998 detected the RPL11 protein (arrow).
Rabbit Anti-rplK Polyclonal Antibody – Cat# MBS645998

Flow Cytometry (FC/FACS) of anti-rplK antibody

Flow Cytometric analysis of Jurkat cells using MBS645998 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-rplK Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS645998

Other Notes

Small volumes of anti-rplK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for rplK purchase

MBS645998 is an antibody targeting rplK. Also known as RPL11, NT (RPL11, 60S ribosomal protein L11, CLL-associated antigen KW-12). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for rplK. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
RpL11
NCBI Official Symbol
rplK
UniProt Protein Name
50S ribosomal protein L11
Protein Family
UniProt Gene Name
rplK
UniProt Synonym Gene Names
rpl11
UniProt Entry Name
RL11_PASMU
NCBI Protein Information
50S ribosomal protein L11

UniProt Comments for rplK

Function: Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors

By similarity. HAMAP-Rule MF_00736

Subunit structure: Part of the ribosomal stalk of the 50S ribosomal subunit. Interacts with L10 and the large rRNA to form the base of the stalk. L10 forms an elongated spine to which L12 dimers bind in a sequential fashion forming a multimeric L10(L12)X complex

By similarity.

Post-translational modification: One or more lysine residues are methylated

By similarity. HAMAP-Rule MF_00736

Sequence similarities: Belongs to the ribosomal protein L11P family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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