AADAT Antibody – Mouse Polyclonal

Function: Transaminase with broad substrate specificity. Has transaminase activity towards aminoadipate, kynurenine, methionine and glutamate. Shows activity also towards tryptophan, aspartate and hydroxykynurenine. Accepts a variety of oxo-acids as amino-group acceptors, with a preference for 2-oxoglutarate, 2-oxocaproic acid, phenylpyruvate and alpha-oxo-gamma-methiol butyric acid. Can also use glyoxylate as amino-group acceptor (in vitro). Ref.5

Catalytic activity: L-kynurenine + 2-oxoglutarate = 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate. Ref.2 Ref.5 Ref.6L-2-aminoadipate + 2-oxoglutarate = 2-oxoadipate + L-glutamate. Ref.2 Ref.5 Ref.6

Cofactor: Pyridoxal phosphate. Ref.2 Ref.6 Ref.7

Enzyme regulation: Kynurenine transaminase activity is competitively inhibited by aminoadipate, asparagine, glutamate, histidine, cysteine, lysine, 3-hydroxy-kynurenine and phenylalanine. Ref.5

Pathway: Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 4/6.

Subunit structure: Homodimer. Ref.5 Ref.6 Ref.7

Subcellular location: Mitochondrion

Potential.

Tissue specificity: Higher expression in the liver. Also found in heart, brain, kidney, pancreas, prostate, testis and ovary.

Sequence similarities: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Biophysicochemical propertiesKinetic parameters:KM=0.9 mM for aminoadipate Ref.5KM=4.7 mM for kynurenineKM=1.7 mM for methionineKM=1.6 mM for glutamateKM=1.8 mM for tyrosineKM=1.2 mM for 2-oxoglutarateKM=1.5 mM for 2-oxocaproic acidKM=1.8 mM for phenylpyruvateKM=1.4 mM for ino-3-pyruvatepH dependence:Optimum pH is 7-9.Temperature dependence:Optimum temperature is 50 degrees Celsius.