Rabbit Anti-RPA4 Polyclonal Antibody – Cat# MBS646008

RPA4 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the RPA4 antibody detected the RPA4 protein (arrow).

RPA4 Antibody – Rabbit Polyclonal

Name: RPA4 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS646008
Price: 895 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS646008

This gene encodes a single-stranded DNA-binding protein that is the 30-kDa subunit of the replication protein A complex. Replication protein A is an essential factor for DNA double-strand break repair and cell cycle checkpoint activation. The encoded protein… More details

$895 / 0.2 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

RPA4, Polyclonal Antibody

Full Product Name

RPA4, CT (RPA4, Replication protein A 30kD subunit, Replication factor A protein 4)

Product Gene Name

anti-RPA4 antibody

Product Synonym Names

Anti -RPA4, CT (RPA4, Replication protein A 30kD subunit, Replication factor A protein 4)

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Purity/Purification

Affinity Purified
Purified by Protein A affinity chromatography.

Specificity

Human

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

28,868 Da

Chromosome Location

Chromosome: X; NC_000023.10 (96138907..96140466). Location: Xq21.33

OMIM

300767

3D Structure

ModBase 3D Structure for Q13156

Immunogen

RPA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic between 233-261 amino acids selected from the C-terminal region of human RPA4.

Product Categories/Family for anti-RPA4 antibody

Antibodies; Abs to DNA Replication; Repair

Applications Tested/Suitable for anti-RPA4 antibody

ELISA (EL/EIA), Western Blot (WB)

Application Notes for anti-RPA4 antibody

Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500

Other Notes

Small volumes of anti-RPA4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RPA4 purchase

MBS646008 is an antibody targeting RPA4. Also known as RPA4, CT (RPA4, Replication protein A 30kD subunit, Replication factor A protein 4). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RPA4. It may not necessarily be applicable to this product.

NCBI GI #

9558731

NCBI GeneID

29935

NCBI Accession #

NP_037479.1

UniProt Primary Accession #

Q13156

UniProt Secondary Accession #

Q3SY03

UniProt Related Accession #

Q13156

GenBank Nucleotide #

NM_013347.4

NCBI Official Full Name

replication protein A 30 kDa subunit

NCBI Official Synonym Full Names

replication protein A4, 30kDa

NCBI Official Symbol

RPA4

NCBI Official Synonym Symbols

HSU24186

UniProt Protein Name

Replication protein A 30 kDa subunit

UniProt Synonym Protein Names

Replication factor A protein 4

Protein Family

Replication protein

UniProt Gene Name

RPA4

UniProt Synonym Gene Names

RP-A p30

UniProt Entry Name

RFA4_HUMAN

NCBI Protein Information

replication protein A 30 kDa subunit; RP-A p30; RF-A protein 4; replication protein A4, 34kDa; replication factor A protein 4; replication protein A complex 34 kd subunit homolog Rpa4

UniProt Comments for RPA4

Function: Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange. Ref.6 Ref.7 Ref.8 Ref.9

Subunit structure: Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. Interacts with RPA1 and RPA3. Ref.1 Ref.6

Subcellular location: Nucleus. Note: Localizes to DNA repair foci after DNA damage. Ref.9

Tissue specificity: Preferentially expressed in placental and colon mucosa. Widely expressed at intermediate or lower levels. Ref.8

Research Articles on RPA4

1. Findings provide the first direct evidence for the function of alternative form of RPA in human DNA metabolism and support a model for aRPA functioning in chromosome maintenance functions in nonproliferating cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RPA4 antibody

Reference Product	PubMed Publications
RPA3 antibody	>6 publications with RPA4 and RPA3
RPA1 antibody	>4 publications with RPA4 and RPA1

Pathways associated with anti-RPA4 antibody

Products by Pathway	Pathway Diagram
Activation Of The Pre-replicative Complex Pathway antibodies	Activation Of The Pre-replicative Complex Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle, Mitotic Pathway antibodies	Cell Cycle, Mitotic Pathway Diagram
DNA Replication Pathway antibodies	DNA Replication Pathway Diagram
DNA Replication Pathway antibodies	DNA Replication Pathway Diagram
DNA Replication Pre-Initiation Pathway antibodies	DNA Replication Pre-Initiation Pathway Diagram
DNA Replication Pathway antibodies	DNA Replication Pathway Diagram
DNA Replication Pathway antibodies	DNA Replication Pathway Diagram
Fanconi Anemia Pathway antibodies	Fanconi Anemia Pathway Diagram
Fanconi Anemia Pathway antibodies	Fanconi Anemia Pathway Diagram

Diseases associated with anti-RPA4 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>2 publications with RPA4 and Neoplasms
Poisoning Antibodies	>2 publications with RPA4 and Poisoning
Nervous System Diseases Antibodies	>1 publications with RPA4 and Nervous System Diseases
Brain Diseases Antibodies	>1 publications with RPA4 and Brain Diseases
Lymphadenitis Antibodies	>1 publications with RPA4 and Lymphadenitis