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DLK1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS646035

DLK1 (Protein delta homolog 1; Preadipocyte factor 1; Pref-1) is a regulator of adipocyte differentiation found in serum and urine. DLK1 might be a decoy ligand. Serum or plasma levels of soluble DLK1 affect negatively or positively adipogenesis and control… More details

$540 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DLK1, Monoclonal Antibody
Full Product Name
DLK1 (Protein Delta Homolog 1, Fetal Antigen 1, Preadipocyte Factor 1, Pref-1, FA1)
Product Gene Name
Product Synonym Names
Anti -DLK1 (Protein Delta Homolog 1, Fetal Antigen 1, Preadipocyte Factor 1, Pref-1, FA1)
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
PF299-1
Host
Mouse
Form/Format
Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human DLK1. Does not cross-react with mouse DLK1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
41,320 Da
Chromosome Location
Chromosome: 12; NC_000078.6 (109452823..109463336). Location: 12 E-F1; 12 60.17 cM
3D Structure
ModBase 3D Structure for Q09163
Immunogen
Recombinant human DLK1 (extracellular domain).
Applications Tested/Suitable for anti-Dlk1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-Dlk1 antibody
Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry, Western Blot

Immunohistochemistry (IHC) of anti-Dlk1 antibody

Immunohistochemical staining of DLK1 MBS646035 in placenta, villi (1:500 dilution).|This antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Mouse Anti-DLK1 Monoclonal Antibody – Cat# MBS646035

Flow Cytometry (FC/FACS) of anti-Dlk1 antibody

FACS: DLK1-transfected HEK 293 (Full length) cells were stained significantly using MBS646035
Mouse Anti-DLK1 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS646035

Western Blot (WB) of anti-Dlk1 antibody

Western blot analysis of human Pref-1using MBS646035 at 1: 2000 dilution:|A.|1. Human Pref-1 Fc protein.|2. Transfected human Pref-1 full length cell lysate (HEK 293).|3. Mock Transfected HEK293 cell lysate.|B.|1. Human Pref-1 Fc protein.|2. Mouse Pref-1 Fc protein.|3. Human DNER Fc protein.|4. Human DLL1 Fc protein.|5. Human DLL3 Fc protein.|6. Human DLL4 Fc protein.|7. Human Jagged1 Fc protein.|8. Human FGF23 Fc protein.
Mouse Anti-DLK1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS646035

Other Notes

Small volumes of anti-Dlk1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Dlk1 purchase

MBS646035 is an antibody targeting Dlk1. Also known as DLK1 (Protein Delta Homolog 1, Fetal Antigen 1, Preadipocyte Factor 1, Pref-1, FA1). Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Dlk1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Dlk1
NCBI Official Synonym Full Names
delta-like 1 homolog (Drosophila)
NCBI Official Symbol
Dlk1
NCBI Official Synonym Symbols
FA1; ZOG; pG2; DlkI; Peg9; SCP1; DLK-1; Ly107; pref-1; AW742678
UniProt Protein Name
Protein delta homolog 1
UniProt Synonym Protein Names
Adipocyte differentiation inhibitor protein; Preadipocyte factor 1
Protein Family
UniProt Gene Name
Dlk1
UniProt Synonym Gene Names
Dlk; Pref1; Scp-1; DLK-1; Pref-1; FA1
UniProt Entry Name
DLK1_MOUSE
NCBI Protein Information
protein delta homolog 1; fetal antigen 1; preadipocyte factor 1; stromal cell derived protein 1; adipocyte differentiation inhibitor protein

UniProt Comments for Dlk1

Function: May have a role in neuroendocrine differentiation. Inhibits adipocyte differentiation. Ref.1 Ref.2

Subunit structure: Monomer.

Subcellular location: Membrane; Single-pass type I membrane protein.

Tissue specificity: Highly expressed in fetal liver, placenta, adult adrenal gland, brain, testis and ovary and, to a lesser degree, in adult kidney, muscle, thymus and heart. Ref.7

Developmental stage: Expression is elevated in liver after birth but starts to decline around postnatal day 16. Ref.7

Post-translational modification: N- and O-glycosylated. Ref.6

Sequence similarities: Contains 6 EGF-like domains.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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