Rabbit Anti-MYLK3 Polyclonal Antibody – Cat# MBS646079

Western Blot analysis in mouse heart lysates (20ug) using MBS646079 (1:2000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution Predicted band size: 86.4kD Blocking/Dilution buffer: 5% NFDM/TBST.

MYLK3 Antibody – Rabbit Polyclonal

Name: MYLK3 Antibody; Polyclonal; Rabbit; Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS646079
Price: 895 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS646079

MBS646079 is an antibody targeting Mylk3. Also known as Mylk3, NT (Mylk3, Putative myosin light chain kinase 3). Reactive in Mouse. Host: Rabbit. Mylk3 may play a role in smooth muscle contraction (By similarity). More details

$895 / 0.2 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Mylk3, Polyclonal Antibody

Full Product Name

Mylk3, NT (Mylk3, Putative myosin light chain kinase 3)

Product Gene Name

anti-Mylk3 antibody

Product Synonym Names

Anti -Mylk3, NT (Mylk3, Putative myosin light chain kinase 3)

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Mouse

Form/Format

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Purity/Purification

Affinity Purified
Purified by Protein A affinity chromatography.

Specificity

Mouse

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

85,541 Da

Chromosome Location

Chromosome: 19; NC_005118.3 (38373230..38407268). Location: 19q11

3D Structure

ModBase 3D Structure for E9PT87

Immunogen

Mouse Mylk3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 173-203 amino acids from the N-terminal region of human Mouse Mylk3.

Product Categories/Family for anti-Mylk3 antibody

Antibodies; Abs to Protein Kinases

Applications Tested/Suitable for anti-Mylk3 antibody

ELISA (EL/EIA), Western Blot (WB)

Application Notes for anti-Mylk3 antibody

Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500

Other Notes

Small volumes of anti-Mylk3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Mylk3. It may not necessarily be applicable to this product.

NCBI GI #

408407655

NCBI GeneID

291926

NCBI Accession #

E9PT87.1

UniProt Primary Accession #

E9PT87

UniProt Secondary Accession #

A8WE96

UniProt Related Accession #

E9PT87

NCBI Official Full Name

Myosin light chain kinase 3

NCBI Official Synonym Full Names

myosin light chain kinase 3

NCBI Official Symbol

Mylk3

NCBI Official Synonym Symbols

MLCK; RGD1305801

UniProt Protein Name

Myosin light chain kinase 3

UniProt Synonym Protein Names

Cardiac-MyBP-C-associated Ca/CaM kinase

Protein Family

Myosin light chain kinase

UniProt Gene Name

Mylk3

UniProt Synonym Gene Names

Cardiac-MLCK

UniProt Entry Name

MYLK3_RAT

NCBI Protein Information

myosin light chain kinase 3; cardiac-MLCK; putative myosin light chain kinase 3; cardiac-MyBP-C-associated Ca/CaM kinase

UniProt Comments for Mylk3

Function: Calmodulin-dependent kinase that phosphorylates MYL2 in vitro. Promotes sarcomere formation in cardiomyocytes. Increases cardiomyocyte contractility. Ref.1 Ref.3

Catalytic activity: ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate.

Cofactor: Magnesium

By similarity.

Subcellular location: Cytoplasm Ref.3.

Tissue specificity: Expressed in cardiomyocytes (at protein level). Up-regulated in heart after experimental myocardial infarction at the mRNA level. Ref.1 Ref.3

Developmental stage: Up-regulated in the heart from 1 week after birth through adulthood. Ref.3

Induction: by NRG1 (at protein level). Ref.1

Post-translational modification: Phosphorylated on serine residues

By similarity.

Sequence similarities: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.Contains 1 protein kinase domain.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Mylk3 antibody

Products by Pathway	Pathway Diagram
Calcium Signaling Pathway antibodies	Calcium Signaling Pathway Diagram
Calcium Signaling Pathway antibodies	Calcium Signaling Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Gastric Acid Secretion Pathway antibodies	Gastric Acid Secretion Pathway Diagram
Gastric Acid Secretion Pathway antibodies	Gastric Acid Secretion Pathway Diagram
Regulation Of Actin Cytoskeleton Pathway antibodies	Regulation Of Actin Cytoskeleton Pathway Diagram
Regulation Of Actin Cytoskeleton Pathway antibodies	Regulation Of Actin Cytoskeleton Pathway Diagram
Vascular Smooth Muscle Contraction Pathway antibodies	Vascular Smooth Muscle Contraction Pathway Diagram
Vascular Smooth Muscle Contraction Pathway antibodies	Vascular Smooth Muscle Contraction Pathway Diagram

Diseases associated with anti-Mylk3 antibody

Disease Name	Pubmed Publications
Heart Diseases Antibodies	>2 publications with Mylk3 and Heart Diseases
Cardiovascular Diseases Antibodies	>2 publications with Mylk3 and Cardiovascular Diseases
Cardiomegaly Antibodies	>1 publications with Mylk3 and Cardiomegaly
Disease Models, Animal Antibodies	>1 publications with Mylk3 and Disease Models, Animal
Hypertrophy Antibodies	>1 publications with Mylk3 and Hypertrophy

Organs/Tissues associated with anti-Mylk3 antibody

Organ/Tissue Name	Pubmed Publications
Heart Antibodies	>3 publications with Mylk3 and Heart