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B2-Microglobulin (HLA-G) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS646087

Beta-2-microglobulin is a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. More details

$880 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
b2-Microglobulin (HLA-G), Monoclonal Antibody
Full Product Name
b2-Microglobulin (B2M, Beta-2-microglobulin, CDABP0092, HDCMA22P)
Product Gene Name
anti-HLA-G antibody
Product Synonym Names
Anti -b2-Microglobulin (B2M, Beta-2-microglobulin, CDABP0092, HDCMA22P)
Immunogen Sequence
MSRSVALAVL ALLSLSGLEA IQRTPKIQVY SRHPAENGKS NFLNCYVSGF HPSDIEVDLL KNGERIEKVE HSDLSFSKDW SFYLLYYTEF TPTEKDEYAC RVNHVTLSQP KIVKWDRDM
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
3F9-2C2
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.4.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Concentration
0.5 mg/ml (lot specific)
Specificity
Recognizes human B2M.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Chromosome Location
Chromosome: 6; NC_000006.11 (29794756..29798899). Location: 6p21.3
OMIM
142871
3D Structure
ModBase 3D Structure for P17693
Immunogen
Full length recombinant corresponding to aa1-119 from human B2M (AAH32589) with GST tag. MW of the GST tag alone is 26kD.

Product Categories/Family for anti-HLA-G antibody

Antibodies; Abs to Serum Proteins
Applications Tested/Suitable for anti-HLA-G antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-HLA-G antibody
Immunohistochemistry (Formalin fixed paraffin embedded)

Western Blot (WB) of anti-HLA-G antibody

Western blot analysis of B2M over-expressed 293 cell line, cotransfected with B2M Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with B2M monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.
Mouse Anti-HLA-G Monoclonal Antibody – Cat# MBS646087

Immunohistochemistry (IHC) of anti-HLA-G antibody

Immunoperoxidase of monoclonal antibody to B2M on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 3ug/ml].
Mouse Anti-HLA-G Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS646087

Immunofluorescence (IF) of anti-HLA-G antibody

Immunofluorescence of monoclonal antibody to B2M on HeLa cell. [antibody concentration 10ug/ml].
Mouse Anti-HLA-G Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS646087

Testing Data of anti-HLA-G antibody

Detection limit for recombinant GST tagged B2M is ~0.03ng/ml as a capture antibody.
Mouse Anti-HLA-G Monoclonal Antibody, Testing Data – Cat# MBS646087

Testing Data of anti-HLA-G antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between CALR and B2M. HeLa cells were stained with CALR rabbit purified polyclonal 1:1200 and B2M mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Mouse Anti-HLA-G Monoclonal Antibody, Testing Data – Cat# MBS646087

Western Blot (WB) of anti-HLA-G antibody

B2M monoclonal antibody, Western Blot analysis of B2M expression in U-2 OS.
Mouse Anti-HLA-G Monoclonal Antibody, Western Blot (WB) – Cat# MBS646087

Western Blot (WB) of anti-HLA-G antibody

Western Blot analysis of B2M expression in transfected 293T cell line by B2M monoclonal antibody. Lane 1: B2M transfected lysate (13.7kD). Lane 2: Non-transfected lysate.
Mouse Anti-HLA-G Monoclonal Antibody, Western Blot (WB) – Cat# MBS646087

Other Notes

Small volumes of anti-HLA-G antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HLA-G purchase

MBS646087 is an antibody targeting HLA-G. Also known as b2-Microglobulin (B2M, Beta-2-microglobulin, CDABP0092, HDCMA22P). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for HLA-G. It may not necessarily be applicable to this product.

NCBI GI #
188468
NCBI GeneID
3135
NCBI Accession #
AAA59836.1
UniProt Primary Accession #
P17693
UniProt Related Accession #
P17693
NCBI Official Full Name
b2 microglobulin
NCBI Official Synonym Full Names
major histocompatibility complex, class I, G
NCBI Official Symbol
HLA-G
NCBI Official Synonym Symbols
MHC-G
UniProt Protein Name
HLA class I histocompatibility antigen, alpha chain G
UniProt Synonym Protein Names
HLA G antigen; MHC class I antigen G
Protein Family
HLA class I histocompatibility antigen
UniProt Gene Name
HLA-G
UniProt Synonym Gene Names
HLA-6.0; HLAG
UniProt Entry Name
HLAG_HUMAN
NCBI Protein Information
HLA class I histocompatibility antigen, alpha chain G; HLA G antigen; b2 microglobulin; HLA class I molecule; MHC class I antigen G; HLA-G histocompatibility antigen, class I, G

NCBI Summary for HLA-G

HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]
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UniProt Comments for HLA-G

Function: Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T-lymphocytes, macrophages and mononuclear cells.

Subunit structure: Heterodimer of an alpha chain and a beta chain (beta-2-microglobulin). Homodimer; disulfide-linked. Binds to LILRB1 and LILRB2. Ref.6 Ref.8

Subcellular location: Membrane; Single-pass type I membrane protein.

Tissue specificity: Expressed in trophoblasts.

Sequence similarities: Belongs to the MHC class I family.Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
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Research Articles on HLA-G

1. This meta-analysis suggests that the 14 bp insertion/deletion HLA-G allele is associated with increased risk of unexplained recurrent spontaneous abortion.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HLA-G antibody

 Reference Product  PubMed Publications
 CD4 antibody  >89 publications with HLA-G and CD4
 IL10 antibody  >75 publications with HLA-G and IL10
 LILRB2 antibody  >40 publications with HLA-G and LILRB2
 KLRC1 antibody  >11 publications with HLA-G and KLRC1
 LIF antibody  >11 publications with HLA-G and LIF
 ADM2 antibody  >2 publications with HLA-G and ADM2
 SPRY2 antibody  >1 publications with HLA-G and SPRY2

Pathways associated with anti-HLA-G antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway antibodies  Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing-Cross Presentation Pathway antibodies  Antigen Processing-Cross Presentation Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram

Diseases associated with anti-HLA-G antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >325 publications with HLA-G and Neoplasms
 Adenocarcinoma Antibodies  >84 publications with HLA-G and Adenocarcinoma
 Skin Diseases Antibodies  >63 publications with HLA-G and Skin Diseases
 Inflammation Antibodies  >61 publications with HLA-G and Inflammation
 Necrosis Antibodies  >43 publications with HLA-G and Necrosis
 Nervous System Diseases Antibodies  >34 publications with HLA-G and Nervous System Diseases
 Gastrointestinal Diseases Antibodies  >31 publications with HLA-G and Gastrointestinal Diseases
 Asthma Antibodies  >27 publications with HLA-G and Asthma
 Breast Neoplasms Antibodies  >24 publications with HLA-G and Breast Neoplasms
 Cardiovascular Diseases Antibodies  >24 publications with HLA-G and Cardiovascular Diseases

Organs/Tissues associated with anti-HLA-G antibody

 Organ/Tissue Name  Pubmed Publications
 Placenta Antibodies  >442 publications with HLA-G and Placenta
 Lung Antibodies  >33 publications with HLA-G and Lung
 Brain Antibodies  >27 publications with HLA-G and Brain
 Thymus Antibodies  >22 publications with HLA-G and Thymus
 Stomach Antibodies  >8 publications with HLA-G and Stomach
 Intestine Antibodies  >7 publications with HLA-G and Intestine
 Testis Antibodies  >5 publications with HLA-G and Testis
 Pancreas Antibodies  >3 publications with HLA-G and Pancreas
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