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NLRC4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS646130

In C. elegans, Ced4 binds and activates Ced3, an apoptotic initiator caspase, via caspase-associated recruitment domains (CARDs). Human Ced4 homologs include APAF1 (MIM 602233), NOD1/CARD4 (MIM 605980), and NOD2/CARD15 (MIM 605956). These proteins have at… More details

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Product Overview

Online Datasheet in PDF
Product Name
NLRC4, Polyclonal Antibody
Full Product Name
NLRC4, NT (NLRC4, CARD12, CLAN, CLAN1, IPAF, NLR family CARD domain-containing protein 4, CARD, LRR, and NACHT-containing protein, Caspase recruitment domain-containing protein 12, Ice protease-activating factor)
Product Gene Name
anti-NLRC4 antibody
Product Synonym Names
Anti -NLRC4, NT (NLRC4, CARD12, CLAN, CLAN1, IPAF, NLR family CARD domain-containing protein 4, CARD, LRR, and NACHT-containing protein, Caspase recruitment domain-containing protein 12, Ice protease-activating factor)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
116,159 Da
Chromosome Location
Chromosome: 2; NC_000002.11 (32449518..32490812, complement). Location: 2p22-p21
OMIM
606831
3D Structure
ModBase 3D Structure for Q9NPP4
Immunogen
NLRC4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-145 amino acids from the N-terminal region of human NLRC4.

Product Categories/Family for anti-NLRC4 antibody

Antibodies; Abs to Apoptosis; Caspase
Applications Tested/Suitable for anti-NLRC4 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-NLRC4 antibody
Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500

Western Blot (WB) of anti-NLRC4 antibody

NLRC4 Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the NLRC4 antibody detected the NLRC4 protein (arrow).
Rabbit Anti-NLRC4 Polyclonal Antibody – Cat# MBS646130

Other Notes

Small volumes of anti-NLRC4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NLRC4 purchase

MBS646130 is an antibody targeting NLRC4. Also known as NLRC4, NT (NLRC4, CARD12, CLAN, CLAN1, IPAF, NLR family CARD domain-containing protein 4, CARD, LRR, and NACHT-containing protein, Caspase recruitment domain-containing protein 12, Ice protease-activating factor). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NLRC4. It may not necessarily be applicable to this product.

NCBI GI #
312433962
NCBI GeneID
58484
NCBI Accession #
NP_001186068.1
UniProt Primary Accession #
Q9NPP4
UniProt Secondary Accession #
Q96J81; Q96J82; Q96J83; A8K9F8; B2RBQ3; B3KTF0; D6W580
UniProt Related Accession #
Q9NPP4
GenBank Nucleotide #
NM_001199139.1
NCBI Official Full Name
NLR family CARD domain-containing protein 4
NCBI Official Synonym Full Names
NLR family, CARD domain containing 4
NCBI Official Symbol
NLRC4
NCBI Official Synonym Symbols
CLAN; IPAF; CLAN1; CLANA; CLANB; CLANC; CLAND; CARD12; CLR2.1
UniProt Protein Name
NLR family CARD domain-containing protein 4
UniProt Synonym Protein Names
CARD, LRR, and NACHT-containing protein; Clan protein; Caspase recruitment domain-containing protein 12; Ice protease-activating factor
Protein Family
NLR family CARD domain-containing protein
UniProt Gene Name
NLRC4
UniProt Synonym Gene Names
CARD12; CLAN; CLAN1; IPAF; Clan protein; Ipaf
UniProt Entry Name
NLRC4_HUMAN
NCBI Protein Information
NLR family CARD domain-containing protein 4; NOD-like receptor C4; ICE-protease activating factor; ice protease-activating factor; CARD, LRR, and NACHT-containing protein; caspase recruitment domain family, member 12; caspase recruitment domain-containing protein 12; nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 4

UniProt Comments for NLRC4

Function: Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis. Ref.11

Subunit structure: Component of the NLRC4 inflammasome

By similarity. Homooligomer. Interacts with ASC, pro-caspase-1, NOD2, BCL10 and NALP1 (NAC) by CARD-CARD interaction. Interacts with EIF2AK2/PKR. Ref.12

Subcellular location: Cytoplasm. Cytoplasm › cytosol

By similarity. Note: Cytoplasmic filaments. Ref.3

Tissue specificity: Isoform 2 is expressed ubiquitously, although highly expressed in lung and spleen. Isoform 1 is highly expressed in lung, followed by leukocytes especially monocytes, lymph node, colon, brain, prostate, placenta, spleen, bone marrow and fetal liver. Isoform 4 is only detected in brain.

Post-translational modification: Phosphorylated at Ser-533 following infection of macrophages with S.typhimurium (Salmonella). Phosphorylation is essential for NLRC4 inflammasome function to promote caspase-1 activation and pyroptosis. PRKCD phosphorylates Ser-533 in vitro

By similarity.

Sequence similarities: Contains 1 CARD domain.Contains 14 LRR (leucine-rich) repeats.Contains 1 NACHT domain.
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Research Articles on NLRC4

1. This review describes the surprisingly diverse mechanisms by which NLRC4 senses bacteria and initiates innate immune responses.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NLRC4 antibody

 Reference Product  PubMed Publications
 CASP1 antibody  >132 publications with NLRC4 and CASP1
 NLRP3 antibody  >89 publications with NLRC4 and NLRP3
 NLRP1 antibody  >31 publications with NLRC4 and NLRP1
 PYCARD antibody  >23 publications with NLRC4 and PYCARD
 NAIP antibody  >16 publications with NLRC4 and NAIP
 NOD2 antibody  >12 publications with NLRC4 and NOD2
 NOD1 antibody  >10 publications with NLRC4 and NOD1
 NLRP4 antibody  >4 publications with NLRC4 and NLRP4

Pathways associated with anti-NLRC4 antibody

 Products by Pathway  Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Inflammasomes Pathway antibodies  Inflammasomes Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Legionellosis Pathway antibodies  Legionellosis Pathway Diagram
 Legionellosis Pathway antibodies  Legionellosis Pathway Diagram
 NOD Pathway antibodies  NOD Pathway Diagram
 NOD-like Receptor Signaling Pathway antibodies  NOD-like Receptor Signaling Pathway Diagram
 NOD-like Receptor Signaling Pathway antibodies  NOD-like Receptor Signaling Pathway Diagram
 Nucleotide-binding Domain, Leucine Rich Repeat Containing Receptor (NLR) Signaling Pathways antibodies  Nucleotide-binding Domain, Leucine Rich Repeat Containing Receptor (NLR) Signaling Pathways Diagram

Diseases associated with anti-NLRC4 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >64 publications with NLRC4 and Inflammation
 Death Antibodies  >61 publications with NLRC4 and Death
 Necrosis Antibodies  >14 publications with NLRC4 and Necrosis
 Immune System Diseases Antibodies  >12 publications with NLRC4 and Immune System Diseases
 Pneumonia Antibodies  >10 publications with NLRC4 and Pneumonia
 Intestinal Diseases Antibodies  >7 publications with NLRC4 and Intestinal Diseases
 Lung Diseases Antibodies  >6 publications with NLRC4 and Lung Diseases
 Autoimmune Diseases Antibodies  >5 publications with NLRC4 and Autoimmune Diseases
 Disease Models, Animal Antibodies  >3 publications with NLRC4 and Disease Models, Animal
 Lung Neoplasms Antibodies  >2 publications with NLRC4 and Lung Neoplasms

Organs/Tissues associated with anti-NLRC4 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >24 publications with NLRC4 and Blood
 Bone Marrow Antibodies  >21 publications with NLRC4 and Bone Marrow
 Liver Antibodies  >6 publications with NLRC4 and Liver
 Brain Antibodies  >5 publications with NLRC4 and Brain
 Thymus Antibodies  >2 publications with NLRC4 and Thymus
 Kidney Antibodies  >2 publications with NLRC4 and Kidney
 Testis Antibodies  >1 publications with NLRC4 and Testis
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