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ENTPD3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS646154

ENTPD3 is similar to E-type nucleotidases (NTPases). NTPases, such as CD39, mediate catabolism of extracellular nucleotides. ENTPD3 contains 4 apyrase-conserved regions which is characteristic of NTPases. More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ENTPD3, Polyclonal Antibody
Full Product Name
ENTPD3, CT (ENTPD3, CD39L3, Ectonucleoside triphosphate diphosphohydrolase 3, CD39 antigen-like 3, Ecto-ATP diphosphohydrolase 3, Ecto-apyrase 3, HB6)
Product Gene Name
Product Synonym Names
Anti -ENTPD3, CT (ENTPD3, CD39L3, Ectonucleoside triphosphate diphosphohydrolase 3, CD39 antigen-like 3, Ecto-ATP diphosphohydrolase 3, Ecto-apyrase 3, HB6)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
59,105 Da
Chromosome Location
Chromosome: 3; NC_000003.11 (40428647..40470110). Location: 3p21.3
OMIM
603161
3D Structure
ModBase 3D Structure for O75355
Immunogen
ENTPD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 499-531 amino acids from the C-terminal region of human ENTPD3.

Product Categories/Family for anti-ENTPD3 antibody

Applications Tested/Suitable for anti-ENTPD3 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-ENTPD3 antibody
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50

Western Blot (WB) of anti-ENTPD3 antibody

Western Blot analysis in K562 and HL-60 cell line lysates (35ug/lane) using MBS646154. This demonstrates that MBS646154 detected the ENTPD3 protein (arrow).
Rabbit Anti-ENTPD3 Polyclonal Antibody – Cat# MBS646154

Immunohistochemistry (IHC) of anti-ENTPD3 antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue using MBS646154. Peroxidase-conjugated secondary antibody and DAB staining were used to visualize the antigen.
Rabbit Anti-ENTPD3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS646154

Flow Cytometry (FC/FACS) of anti-ENTPD3 antibody

Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat anti-rabbit secondary antibody was used.
Rabbit Anti-ENTPD3 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS646154

Other Notes

Small volumes of anti-ENTPD3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ENTPD3 purchase

MBS646154 is an antibody targeting ENTPD3. Also known as ENTPD3, CT (ENTPD3, CD39L3, Ectonucleoside triphosphate diphosphohydrolase 3, CD39 antigen-like 3, Ecto-ATP diphosphohydrolase 3, Ecto-apyrase 3, HB6). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ENTPD3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
ENTPD3 protein
NCBI Official Synonym Full Names
ectonucleoside triphosphate diphosphohydrolase 3
NCBI Official Symbol
ENTPD3
NCBI Official Synonym Symbols
HB6; CD39L3; NTPDase-3
UniProt Protein Name
Ectonucleoside triphosphate diphosphohydrolase 3
UniProt Synonym Protein Names
CD39 antigen-like 3; Ecto-ATP diphosphohydrolase 3; Ecto-ATPDase 3; Ecto-ATPase 3; Ecto-apyrase 3; HB6
UniProt Gene Name
ENTPD3
UniProt Synonym Gene Names
CD39L3; NTPDase 3; Ecto-ATPDase 3
UniProt Entry Name
ENTP3_HUMAN
NCBI Protein Information
ectonucleoside triphosphate diphosphohydrolase 3; NTPDase 3; CD39-like 3; ecto-ATPase 3; ecto-ATPDase 3; ecto-apyrase 3; CD39 antigen-like 3; ecto-ATP diphosphohydrolase 3

UniProt Comments for ENTPD3

Function: Has a threefold preference for the hydrolysis of ATP over ADP.

Catalytic activity: A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

Cofactor: Ca2+ or Mg2+.

Subcellular location: Membrane; Multi-pass membrane protein

Potential.

Tissue specificity: Expressed in adult brain, pancreas, spleen and prostate. Moderate or low expression is seen in most tissues. Not expressed in liver and peripheral blood leukocytes.

Sequence similarities: Belongs to the GDA1/CD39 NTPase family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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