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RHOXF2B Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS646184

MBS646184 is an antibody targeting RHOXF2B. Also known as RHOXF2B, ID (RHOXF2B, Rhox homeobox family member 2B). Reactive in Human. Host: Rabbit. The function of this protein remains unknown. More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RHOXF2B, Polyclonal Antibody
Full Product Name
RHOXF2B, ID (RHOXF2B, Rhox homeobox family member 2B)
Product Gene Name
Product Synonym Names
Anti -RHOXF2B, ID (RHOXF2B, Rhox homeobox family member 2B)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
31,637 Da
Chromosome Location
Chromosome: X; NC_000023.10 (119206229..119211707, complement). Location: Xq24
3D Structure
ModBase 3D Structure for P0C7M4
Immunogen
RHOXF2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 94-121 amino acids from the Central region of human RHOXF2B.
Applications Tested/Suitable for anti-RHOXF2B antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-RHOXF2B antibody
Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500

Western Blot (WB) of anti-RHOXF2B antibody

RHOXF2B Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the RHOXF2B antibody detected the RHOXF2B protein (arrow).
Rabbit Anti-RHOXF2B Polyclonal Antibody – Cat# MBS646184

Immunofluorescence (IF) of anti-RHOXF2B antibody

Fluorescent confocal image of A549 cell stained with RHOXF2B Antibody (Center) .A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RHOXF2B primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).RHOXF2B immunoreactivity is localized to Nucleus significantly.
Rabbit Anti-RHOXF2B Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS646184

Other Notes

Small volumes of anti-RHOXF2B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RHOXF2B. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
rhox homeobox family member 2B
NCBI Official Synonym Full Names
Rhox homeobox family, member 2B
NCBI Official Symbol
RHOXF2B
UniProt Protein Name
Rhox homeobox family member 2B
Protein Family
UniProt Gene Name
RHOXF2B
UniProt Entry Name
RHF2B_HUMAN
NCBI Protein Information
rhox homeobox family member 2B

UniProt Comments for RHOXF2B

Subcellular location: Nucleus

By similarity.

Sequence similarities: Belongs to the paired-like homeobox family. PEPP subfamily.Contains 1 homeobox DNA-binding domain.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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