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AMT Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6462833

MBS6462833 is an antibody targeting AMT. Also known as AMT, NT (Aminomethyltransferase, Mitochondrial, Glycine Cleavage System T Protein, GCVT, GCST) (MaxLight 490). Reactive in Human. Host: Rabbit. More details

$1060 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
AMT, Polyclonal Antibody
Full Product Name
AMT, NT (Aminomethyltransferase, Mitochondrial, Glycine Cleavage System T Protein, GCVT, GCST) (MaxLight 490)
Product Gene Name
anti-AMT antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Specificity
Recognizes human AMT.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
43.3kDa (398aa) confirmed by MALDI-TOF
OMIM
238310
3D Structure
ModBase 3D Structure for P48728
Immunogen
Synthetic peptide selected from the N-terminal region of human AMT (KLH).
Conjugate
MaxLight490
Note
Preservative Free

Product Categories/Family for anti-AMT antibody

Antibodies; Enzymes
Applications Tested/Suitable for anti-AMT antibody
Western Blot (WB), FLISA
Application Notes for anti-AMT antibody
WB: 1:50-1:100
FLISA: 1:1,000
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-AMT antibody

Western blot analysis of AMT Antibody (N-term) in HepG2 cell line lysates (35ug/lane). AMT (arrow) was detected using the purified Pab.
Rabbit Anti-AMT Polyclonal Antibody – Cat# MBS6462833

Immunohistochemistry (IHC) of anti-AMT antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with AMT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-AMT Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6462833

Flow Cytometry (FC/FACS) of anti-AMT antibody

AMT Antibody (N-term) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-AMT Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6462833

Product References and Citations for anti-AMT antibody

1. Nanao, K., Genomics 19(1), 27-30 (1994).

Other Notes

Small volumes of anti-AMT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for AMT. It may not necessarily be applicable to this product.

NCBI GI #
44662838
NCBI GeneID
275
NCBI Accession #
NP_000472
UniProt Primary Accession #
P48728
UniProt Secondary Accession #
Q96IG6; A8K3I5; B4DE61; B4DJQ0; E9PBG1
UniProt Related Accession #
P48728
GenBank Nucleotide #
NM_000481.3
NCBI Official Full Name
aminomethyltransferase, mitochondrial isoform 1
NCBI Official Synonym Full Names
aminomethyltransferase
NCBI Official Symbol
AMT
NCBI Official Synonym Symbols
GCE; NKH; GCST; GCVT
UniProt Protein Name
Aminomethyltransferase, mitochondrial
UniProt Synonym Protein Names
Glycine cleavage system T protein; GCVT
Protein Family
Probable ammonia channel
UniProt Gene Name
AMT
UniProt Synonym Gene Names
GCST; GCVT
UniProt Entry Name
GCST_HUMAN
NCBI Protein Information
aminomethyltransferase, mitochondrial

NCBI Summary for AMT

This gene encodes one of four critical components of the glycine cleavage system. Mutations in this gene have been associated with glycine encephalopathy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

UniProt Comments for AMT

AMT: The glycine cleavage system catalyzes the degradation of glycine. Defects in AMT are a cause of non-ketotic hyperglycinemia (NKH); also known as glycine encephalopathy (GCE). NKH is an autosomal recessive disease characterized by accumulation of a large amount of glycine in body fluid and by severe neurological symptoms. Belongs to the GcvT family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - glycine, serine and threonine; Cofactor and Vitamin Metabolism - one carbon pool by folate; EC 2.1.2.10; Energy Metabolism - nitrogen; Methyltransferase; Mitochondrial

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: mitochondrial matrix; mitochondrion

Molecular Function: aminomethyltransferase activity

Biological Process: glycine catabolic process; glycine decarboxylation via glycine cleavage system; glyoxylate metabolic process

Disease: Glycine Encephalopathy
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Research Articles on AMT

1. The position and frequency of the breakpoint for CNVs correlated with intron size and presence of Alu elements. Missense mutations, most often recurring, were the most common type of disease-causing mutation in AMT

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-AMT antibody

 Reference Product  PubMed Publications
 GLDC antibody  >16 publications with AMT and GLDC
 GCSH antibody  >14 publications with AMT and GCSH
 DLD antibody  >2 publications with AMT and DLD
 SHMT2 antibody  >1 publications with AMT and SHMT2

Pathways associated with anti-AMT antibody

 Products by Pathway  Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Glycine Degradation Pathway antibodies  Glycine Degradation Pathway Diagram
 Glycine, Serine And Threonine Metabolism Pathway antibodies  Glycine, Serine And Threonine Metabolism Pathway Diagram
 Glycine, Serine And Threonine Metabolism Pathway antibodies  Glycine, Serine And Threonine Metabolism Pathway Diagram
 Glyoxylate And Dicarboxylate Metabolism Pathway antibodies  Glyoxylate And Dicarboxylate Metabolism Pathway Diagram
 Glyoxylate And Dicarboxylate Metabolism Pathway antibodies  Glyoxylate And Dicarboxylate Metabolism Pathway Diagram
 Glyoxylate Metabolism And Glycine Degradation Pathway antibodies  Glyoxylate Metabolism And Glycine Degradation Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram

Diseases associated with anti-AMT antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >312 publications with AMT and Nervous System Diseases
 Brain Diseases Antibodies  >132 publications with AMT and Brain Diseases
 Cardiovascular Diseases Antibodies  >105 publications with AMT and Cardiovascular Diseases
 Inflammation Antibodies  >101 publications with AMT and Inflammation
 Vascular Diseases Antibodies  >84 publications with AMT and Vascular Diseases
 Heart Diseases Antibodies  >55 publications with AMT and Heart Diseases
 Depressive Disorder Antibodies  >50 publications with AMT and Depressive Disorder
 Memory Disorders Antibodies  >49 publications with AMT and Memory Disorders
 Disease Models, Animal Antibodies  >44 publications with AMT and Disease Models, Animal
 Cognition Disorders Antibodies  >38 publications with AMT and Cognition Disorders

Organs/Tissues associated with anti-AMT antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >369 publications with AMT and Blood
 Brain Antibodies  >300 publications with AMT and Brain
 Eye Antibodies  >198 publications with AMT and Eye
 Embryonic Tissue Antibodies  >185 publications with AMT and Embryonic Tissue
 Muscle Antibodies  >134 publications with AMT and Muscle
 Vascular Antibodies  >80 publications with AMT and Vascular
 Bone Antibodies  >79 publications with AMT and Bone
 Liver Antibodies  >73 publications with AMT and Liver
 Heart Antibodies  >65 publications with AMT and Heart
 Kidney Antibodies  >49 publications with AMT and Kidney
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