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kdm1a Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS646290

LSD1 is a histone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. LSD1 contains a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
LSD1 (kdm1a), Polyclonal Antibody
Full Product Name
LSD1, CT (KDM1A, AOF2, KDM1, KIAA0601, LSD1, Lysine-specific histone demethylase 1A, BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2)
Product Gene Name
anti-kdm1a antibody
Product Synonym Names
Anti -LSD1, CT (KDM1A, AOF2, KDM1, KIAA0601, LSD1, Lysine-specific histone demethylase 1A, BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Purified
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Chromosome Location
Chromosome: 17; NC_007128.5 (28143505..28161117, complement). Location: chromosome: 17
Immunogen
LSD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human LSD1.

Product Categories/Family for anti-kdm1a antibody

Antibodies; Abs to Enzymes; Oxidase
Applications Tested/Suitable for anti-kdm1a antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-kdm1a antibody
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western BLot 1:100-500
Immunohistochemistry: 1:50-100

Western Blot (WB) of anti-kdm1a antibody

Western Blot analysis in 293 cell lysates (2ug/lane) using MBS646290. Lane 1: Nontransfected Lane 2: Transiently transfected with the AOF2 gene.
Rabbit Anti-kdm1a Polyclonal Antibody – Cat# MBS646290

Western Blot (WB) of anti-kdm1a antibody

Western Blot of nuclear extracts of control and PhIP-treated HMEC. Proteins were transferred onto polyvinylidene difluoride and blotted with MBS646290. Nuclear LSD1 protein levels increased in carcinogen-treated HMEC compared with control HMEC.
Rabbit Anti-kdm1a Polyclonal Antibody, Western Blot (WB) – Cat# MBS646290

Immunohistochemistry (IHC) of anti-kdm1a antibody

Immunohistochemistry analysis of formalin-fixed and paraffin-embedded human cancer tissue using MBS646290 followed by peroxidase-conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS646290 for immunohistochemistry
Rabbit Anti-kdm1a Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS646290

Immunofluorescence (IF) of anti-kdm1a antibody

Confocal immunofluorescent analysis of 293 cells using MBS646290 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Rabbit Anti-kdm1a Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS646290

Other Notes

Small volumes of anti-kdm1a antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for kdm1a purchase

MBS646290 is an antibody targeting kdm1a. Also known as LSD1, CT (KDM1A, AOF2, KDM1, KIAA0601, LSD1, Lysine-specific histone demethylase 1A, BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for kdm1a. It may not necessarily be applicable to this product.

NCBI GI #
339715214
NCBI GeneID
558450
NCBI Accession #
NP_001229924.1
GenBank Nucleotide #
NM_001242995.1
NCBI Official Full Name
lysine-specific histone demethylase 1A
NCBI Official Synonym Full Names
lysine (K)-specific demethylase 1a
NCBI Official Symbol
kdm1a
NCBI Official Synonym Symbols
aof2; kdm1; wu:fb82e06; wu:fc51a02; wu:fj36a05; wu:fj66b06
Protein Family
Lysine-specific histone demethylase
NCBI Protein Information
lysine-specific histone demethylase 1A; lsd1; lysine (K)-specific demethylase 1; amine oxidase (flavin containing) domain 2

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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