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Steroidogenic Acute Regulatory (STAR) ELISA Kit for Rat

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS006463

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated STAR concentrations in Rat serum, plasma and other body fluids. Using Purified Rat STAR antibody to coat Microelisa Stripplate wells to… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Steroidogenic Acute Regulatory Protein (STAR), ELISA Kit
Full Product Name
Rat Steroidogenic Acute Regulatory Protein ELISA Kit
Product Gene Name
STAR elisa kit
Species Reactivity
Rat
Assay Type
Sandwich
Samples
Serum, Plasma, Tissue Homogenate, Feces and Urine
Detection Range
31.2mumol/L-1000mumol/L.
Sensitivity
5.0mumol/L.
Specificity
No significant cross-reactivity or interference between Rat STAR and analogues was observed.
Preparation and Storage
Store all reagents at 2-8 degree C
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Molecular Weight
31,914 Da
Chromosome Location
Chromosome: 8; NC_000008.10 (38000218..38008600, complement). Location: 8p11.2
3D Structure
ModBase 3D Structure for Q6IBK0
Intended Uses
This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated STAR concentrations in Rat serum, plasma and other body fluids. Using Purified Rat STAR antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add STAR and STAR antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of STAR in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Other Notes

Small volumes of STAR elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for STAR purchase

MBS006463 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Steroidogenic Acute Regulatory Protein (STAR) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing STAR. The ELISA analytical biochemical technique of the MBS006463 kit is based on STAR antibody-STAR antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect STAR antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, STAR. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for STAR. It may not necessarily be applicable to this product.

NCBI GI #
49456655
NCBI GeneID
6770
NCBI Accession #
CAG46648.1
UniProt Primary Accession #
Q6IBK0
UniProt Related Accession #
P49675
NCBI Official Full Name
STAR
NCBI Official Synonym Full Names
steroidogenic acute regulatory protein
NCBI Official Symbol
STAR
NCBI Official Synonym Symbols
STARD1
UniProt Protein Name
STAR protein
Protein Family
Steroidogenic acute regulatory protein
UniProt Gene Name
STAR
UniProt Entry Name
Q6IBK0_HUMAN
NCBI Protein Information
steroidogenic acute regulatory protein, mitochondrial; cholesterol trafficker; START domain containing 1; steroid acute regulatory protein; START domain-containing protein 1; mitochondrial steroid acute regulatory protein; StAR-related lipid transfer (START) domain containing 1

NCBI Summary for STAR

The protein encoded by this gene plays a key role in the acute regulation of steroid hormone synthesis by enhancing the conversion of cholesterol into pregnenolone. This protein permits the cleavage of cholesterol into pregnenolone by mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane. Mutations in this gene are a cause of congenital lipoid adrenal hyperplasia (CLAH), also called lipoid CAH. A pseudogene of this gene is located on chromosome 13. [provided by RefSeq, Jul 2008]
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UniProt Comments for STAR

STAR: Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone. May interact with TSPO. Expressed in gonads, adrenal cortex and kidney.

Protein type: Mitochondrial; Carrier; Lipid-binding

Chromosomal Location of Human Ortholog: 8p11.2

Cellular Component: mitochondrial crista; neuron projection; cell soma; mitochondrial intermembrane space; cytosol

Molecular Function: cholesterol transporter activity; cholesterol binding

Biological Process: response to nicotine; steroid metabolic process; diterpenoid metabolic process; dibenzo-p-dioxin metabolic process; regulation of steroid biosynthetic process; estrogen biosynthetic process; biphenyl metabolic process; circadian sleep/wake cycle, REM sleep; negative regulation of neuron apoptosis; response to nutrient; response to corticosterone stimulus; response to drug; bile acid biosynthetic process; cholesterol metabolic process; positive regulation of neurogenesis; progesterone biosynthetic process; insecticide metabolic process; fractalkine metabolic process; male gonad development; phthalate metabolic process; intracellular cholesterol transport; response to herbicide; glucocorticoid metabolic process; response to ethanol; cellular response to insulin stimulus; response to hydrogen peroxide; response to estrogen stimulus; response to lead ion; C21-steroid hormone biosynthetic process; response to ionizing radiation; brain development; response to activity; steroid biosynthetic process; regulation of neuronal synaptic plasticity; phenol metabolic process

Disease: Lipoid Congenital Adrenal Hyperplasia
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Research Articles on STAR

1. deficiencies in the steroidogenic acute regulatory protein and the cholesterol side chain cleavage enzyme cause neonatal adrenal failure

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with STAR elisa kit

 Products by Pathway  Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Metabolism Of Steroid Hormones And Vitamin D Pathway antibodies  Metabolism Of Steroid Hormones And Vitamin D Pathway Diagram
 Ovarian Steroidogenesis Pathway antibodies  Ovarian Steroidogenesis Pathway Diagram
 Ovarian Steroidogenesis Pathway antibodies  Ovarian Steroidogenesis Pathway Diagram
 Pregnenolone Biosynthesis Pathway antibodies  Pregnenolone Biosynthesis Pathway Diagram

Diseases associated with STAR elisa kit

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >694 publications with STAR and Neoplasms
 Nervous System Diseases Antibodies  >496 publications with STAR and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >354 publications with STAR and Cardiovascular Diseases
 Drug Toxicity Antibodies  >283 publications with STAR and Drug Toxicity
 Brain Diseases Antibodies  >277 publications with STAR and Brain Diseases
 Inflammation Antibodies  >168 publications with STAR and Inflammation
 Heart Diseases Antibodies  >163 publications with STAR and Heart Diseases
 Kidney Diseases Antibodies  >162 publications with STAR and Kidney Diseases
 Breast Neoplasms Antibodies  >158 publications with STAR and Breast Neoplasms
 Hyperplasia Antibodies  >148 publications with STAR and Hyperplasia

Organs/Tissues associated with STAR elisa kit

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >638 publications with STAR and Brain
 Ovary Antibodies  >470 publications with STAR and Ovary
 Testis Antibodies  >411 publications with STAR and Testis
 Eye Antibodies  >408 publications with STAR and Eye
 Kidney Antibodies  >268 publications with STAR and Kidney
 Adrenal Gland Antibodies  >254 publications with STAR and Adrenal Gland
 Muscle Antibodies  >239 publications with STAR and Muscle
 Skin Antibodies  >211 publications with STAR and Skin
 Lung Antibodies  >149 publications with STAR and Lung
 Embryonic Tissue Antibodies  >145 publications with STAR and Embryonic Tissue
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