Rabbit Anti-UBE2M Monoclonal Antibody – Cat# MBS646307

Western blot analysis of extracts from various cell lines using MBS646307.

UBE2M Antibody – Rabbit Monoclonal

Name: UBE2M Antibody; Monoclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS646307
Price: 815 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS646307

Similar to ubiquitin, Nedd8 signals to target proteins through an enzymatic cascade composed of Nedd8-specific E1 (activating)- and E2 (conjugating)-enzymes (1,2). The E2 ligase specific for Nedd8 is Ubc12 (3-5). Ubc12 forms a heterodimeric conjugate with… More details

$815 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

UBE2M, Monoclonal Antibody

Full Product Name

UBE2M (Ubiquitin-conjugating Enzyme E2 M, Ubiquitin-protein Ligase M, Ubiquitin Carrier Protein M, Nedd8-conjugating Enzyme Ubc12, NEDD8 Protein Ligase, NEDD8 Carrier Protein, UBC12)

Product Gene Name

anti-UBE2M antibody

Product Synonym Names

Anti -UBE2M (Ubiquitin-conjugating Enzyme E2 M, Ubiquitin-protein Ligase M, Ubiquitin Carrier Protein M, Nedd8-conjugating Enzyme Ubc12, NEDD8 Protein Ligase, NEDD8 Carrier Protein, UBC12)

Clonality

Monoclonal

Isotype

IgG

Clone Number

D13D7

Host

Rabbit

Species Reactivity

Human, Monkey, Mouse, Rat

Form/Format

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol.

Purity/Purification

Supernatant
Supernatant

Specificity

Recognizes endogeneous levels of total human Ubc12. Species Crossreactivity: Monkey, mouse and rat.

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

20,900 Da

Chromosome Location

Chromosome: 18; AC_000175.1 (65964092..65967276, complement). Location: chromosome: 18

3D Structure

ModBase 3D Structure for A3KN22

Immunogen

Synthetic peptide corresponding to residues surrounding Lys36 of human Ubc12.

Product Categories/Family for anti-UBE2M antibody

Antibodies; Abs to Ubiquitin Ligases

Applications Tested/Suitable for anti-UBE2M antibody

Western Blot (WB), Immunoprecipitation (IP)

Application Notes for anti-UBE2M antibody

Suitable for use in Western Blot and Immunoprecipitation.
Dilution: Western Blot: 1:1000
Immunoprecipitation: 1:50

Other Notes

Small volumes of anti-UBE2M antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for UBE2M purchase

MBS646307 is an antibody targeting UBE2M. Also known as UBE2M (Ubiquitin-conjugating Enzyme E2 M, Ubiquitin-protein Ligase M, Ubiquitin Carrier Protein M, Nedd8-conjugating Enzyme Ubc12, NEDD8 Protein Ligase, NEDD8 Carrier Protein, UBC12). Reactive in Human, Monkey, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for UBE2M. It may not necessarily be applicable to this product.

NCBI GI #

126920920

NCBI GeneID

613343

NCBI Accession #

AAI33502.1

UniProt Primary Accession #

A3KN22

UniProt Related Accession #

A3KN22

NCBI Official Full Name

UBE2M protein

NCBI Official Synonym Full Names

ubiquitin-conjugating enzyme E2M

NCBI Official Symbol

UBE2M

UniProt Protein Name

NEDD8-conjugating enzyme Ubc12

UniProt Synonym Protein Names

NEDD8 carrier protein; NEDD8 protein ligase; Ubiquitin-conjugating enzyme E2 M

UniProt Gene Name

UBE2M

UniProt Synonym Gene Names

UBC12

UniProt Entry Name

UBC12_BOVIN

NCBI Protein Information

NEDD8-conjugating enzyme Ubc12; NEDD8 protein ligase; NEDD8 carrier protein; ubiquitin-conjugating enzyme E2 M; ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)

UniProt Comments for UBE2M

Function: Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation

By similarity.

Catalytic activity: ATP + NEDD8 + protein lysine = AMP + diphosphate + protein N-NEDD8yllysine.

Pathway: Protein modification; protein neddylation.

Subunit structure: Interacts with UBA3, DCN1L and RBX1

By similarity.

Domain: Both the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8

By similarity.

Post-translational modification: The acetylation of Met-1 increases affinity for DCUN1D1 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins

By similarity.

Sequence similarities: Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-UBE2M antibody

Products by Pathway	Pathway Diagram
Ubiquitin Mediated Proteolysis Pathway antibodies	Ubiquitin Mediated Proteolysis Pathway Diagram
Ubiquitin Mediated Proteolysis Pathway antibodies	Ubiquitin Mediated Proteolysis Pathway Diagram

Diseases associated with anti-UBE2M antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>2 publications with UBE2M and Neoplasms
Urinary Bladder Neoplasms Antibodies	>1 publications with UBE2M and Urinary Bladder Neoplasms