Rabbit Anti-APH1 Polyclonal Antibody – Cat# MBS6463315

APH1 Antibody – Rabbit Polyclonal

Name: APH1 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS6463315
Price: 1060 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6463315

MBS6463315 is an antibody targeting APH1. Also known as APH1, NT (Gamma-secretase Subunit APH-1A, APH-1a, APH-1a, Aph-1alpha, Presenilin-stabilization Factor, PSF, CGI-78, UNQ579/PRO1141) (MaxLight 650). Reactive in Human. Host: Rabbit. More details

$1060 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

APH1, Polyclonal Antibody

Full Product Name

APH1, NT (Gamma-secretase Subunit APH-1A, APH-1a, APH-1a, Aph-1alpha, Presenilin-stabilization Factor, PSF, CGI-78, UNQ579/PRO1141) (MaxLight 650)

Product Gene Name

anti-APH1 antibody

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

Purity/Purification

Purified by Protein G Affinity Chromatography.

Specificity

Recognizes human APH1.

Preparation and Storage

Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Molecular Weight

28.996

OMIM

607629

3D Structure

ModBase 3D Structure for Q96BI3

Immunogen

Synthetic peptide selected from the N-terminal region of human APH1 (KLH).

Conjugate

MaxLight650

Note

Preservative Free

Product Categories/Family for anti-APH1 antibody

Antibodies; Membrane Proteins

Applications Tested/Suitable for anti-APH1 antibody

Immunohistochemistry (IHC), FLISA, Western Blot (WB)

Application Notes for anti-APH1 antibody

IHC: 1:50-1:100
FLISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.

Product References and Citations for anti-APH1 antibody

1. Ota, T., et al., Nat. Genet. 36(1):40-45 (2004). 2. Clark, H.F., et al., Genome Res. 13(10):2265-2270 (2003). 3. Marlow, L., et al., Biochem. Biophys. Res. Commun. 305(3):502-509 (2003). 4. Kimberly, W.T., et al., Proc. Natl. Acad. Sci. USA 100(11):6382-6387 (2003). 5. Edbauer, D., et al., Nat. Cell Biol. 5(5):486-488 (2003).

Other Notes

Small volumes of anti-APH1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for APH1. It may not necessarily be applicable to this product.

NCBI GI #

117606362

NCBI GeneID

51107

NCBI Accession #

NP_001071096

NCBI Related Accession #

NP_057106

UniProt Primary Accession #

Q96BI3

UniProt Related Accession #

Q96BI3

GenBank Nucleotide #

NP_001071096.1

NCBI Official Full Name

gamma-secretase subunit APH-1A isoform 1

NCBI Official Synonym Full Names

aph-1 homolog A, gamma-secretase subunit

NCBI Official Symbol

APH1A

NCBI Official Synonym Symbols

APH-1; APH-1A; CGI-78; 6530402N02Rik

UniProt Protein Name

Gamma-secretase subunit APH-1A

UniProt Synonym Protein Names

Aph-1alpha; Presenilin-stabilization factor

Protein Family

Bis(5'-nucleosyl)-tetraphosphatase

UniProt Gene Name

APH1A

UniProt Synonym Gene Names

PSF; APH-1a

UniProt Entry Name

APH1A_HUMAN

NCBI Protein Information

gamma-secretase subunit APH-1A

NCBI Summary for APH1

This gene encodes a component of the gamma secretase complex that cleaves integral membrane proteins such as Notch receptors and beta-amyloid precursor protein. The gamma secretase complex contains this gene product, or the paralogous anterior pharynx defective 1 homolog B (APH1B), along with the presenilin, nicastrin, and presenilin enhancer-2 proteins. The precise function of this seven-transmembrane-domain protein is unknown though it is suspected of facilitating the association of nicastrin and presenilin in the gamma secretase complex as well as interacting with substrates of the gamma secretase complex prior to their proteolytic processing. Polymorphisms in a promoter region of this gene have been associated with an increased risk for developing sporadic Alzheimer's disease. Alternative splicing results in multiple protein-coding and non-protein-coding transcript variants. [provided by RefSeq, Aug 2011]

UniProt Comments for APH1

APH1A: Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex. Belongs to the APH-1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Membrane protein, multi-pass; Endoplasmic reticulum; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p36.13-q31.3

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; endopeptidase activity

Biological Process: positive regulation of catalytic activity; axon guidance; Notch signaling pathway; amyloid precursor protein catabolic process; membrane protein intracellular domain proteolysis; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; membrane protein ectodomain proteolysis; ephrin receptor signaling pathway; Notch receptor processing; protein processing; metanephros development

Research Articles on APH1

1. Using purified PSEN1/Aph1A gamma-secretase and the APPC99-3XFLAG substrate, authors show that substrate shortening progressively destabilizes the consecutive enzyme-substrate complexes that characterize the sequential gamma-secretase processing of APP; present a unifying model for how PSEN or APP mutations enhance amyloidogenic Abeta production, suggests that environmental factors may increase Alzheimer's Disease risk.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-APH1 antibody

Reference Product	PubMed Publications
PSENEN antibody	>44 publications with APH1 and PSENEN
PSEN1 antibody	>39 publications with APH1 and PSEN1
BACE1 antibody	>37 publications with APH1 and BACE1
APP antibody	>30 publications with APH1 and APP
PSEN2 antibody	>11 publications with APH1 and PSEN2
NOTCH1 antibody	>3 publications with APH1 and NOTCH1

Pathways associated with anti-APH1 antibody

Products by Pathway	Pathway Diagram
Activated NOTCH1 Transmits Signal To The Nucleus Pathway antibodies	Activated NOTCH1 Transmits Signal To The Nucleus Pathway Diagram
Alzheimer's Disease Pathway antibodies	Alzheimer's Disease Pathway Diagram
Alzheimer's Disease Pathway antibodies	Alzheimer's Disease Pathway Diagram
Alzheimers Disease Pathway antibodies	Alzheimers Disease Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Cell Death Signalling Via NRAGE, NRIF And NADE Pathway antibodies	Cell Death Signalling Via NRAGE, NRIF And NADE Pathway Diagram
Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway antibodies	Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway Diagram
Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway antibodies	Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway Diagram
Delta-Notch Signaling Pathway antibodies	Delta-Notch Signaling Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram

Diseases associated with anti-APH1 antibody

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>29 publications with APH1 and Nervous System Diseases
Vascular Diseases Antibodies	>3 publications with APH1 and Vascular Diseases
Cardiovascular Diseases Antibodies	>3 publications with APH1 and Cardiovascular Diseases
Neurologic Manifestations Antibodies	>1 publications with APH1 and Neurologic Manifestations
Atherosclerosis Antibodies	>1 publications with APH1 and Atherosclerosis
Weight Loss Antibodies	>1 publications with APH1 and Weight Loss
Memory Disorders Antibodies	>1 publications with APH1 and Memory Disorders
Learning Disorders Antibodies	>1 publications with APH1 and Learning Disorders
Disease Models, Animal Antibodies	>1 publications with APH1 and Disease Models, Animal
Necrosis Antibodies	>1 publications with APH1 and Necrosis

Organs/Tissues associated with anti-APH1 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>22 publications with APH1 and Brain
Pharynx Antibodies	>18 publications with APH1 and Pharynx
Vascular Antibodies	>4 publications with APH1 and Vascular
Blood Antibodies	>3 publications with APH1 and Blood
Embryonic Tissue Antibodies	>2 publications with APH1 and Embryonic Tissue
Kidney Antibodies	>2 publications with APH1 and Kidney
Nerve Antibodies	>2 publications with APH1 and Nerve
Ovary Antibodies	>1 publications with APH1 and Ovary
Connective Tissue Antibodies	>1 publications with APH1 and Connective Tissue
Testis Antibodies	>1 publications with APH1 and Testis