Mouse Anti-NDFIP2 Polyclonal Antibody – Cat# MBS646335

Western Blot analysis of NDFIP2 expression in transfected 293T cell line by NDFIP2 polyclonal antibody. Lane 1: NDFIP2 transfected lysate (26.62kD). Lane 2: Non-transfected lysate.

NDFIP2 Antibody – Mouse Polyclonal

Name: NDFIP2 Antibody; Polyclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS646335
Price: 880 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS646335

Activates HECT domain-containing E3 ubiquitin-protein ligases, including ITCH, NEDD4, NEDD4L, SMURF2, WWP1 and WWP2, and consequently modulates the stability of their targets. As a result, may control many cellular processes. Recruits ITCH, NEDD4 and SMURF2… More details

$880 / 0.05 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

NDFIP2, Polyclonal Antibody

Full Product Name

NDFIP2 (NEDD4 Family-interacting Protein 2, NEDD4 WW Domain-binding Protein 5A, Putative MAPK-activating Protein PM04/PM05/PM06/PM07, Putative NF-kappa-B-activating Protein 413, KIAA1165, N4WBP5A, FLJ25842)

Product Gene Name

anti-NDFIP2 antibody

Product Synonym Names

Anti -NDFIP2 (NEDD4 Family-interacting Protein 2, NEDD4 WW Domain-binding Protein 5A, Putative MAPK-activating Protein PM04/PM05/PM06/PM07, Putative NF-kappa-B-activating Protein 413, KIAA1165, N4WBP5A, FLJ25842)

Immunogen Sequence

MDHHQPGTGR YQVLLNEEDN SESSAIEQPP TSNPAPQIVQ AVSSAPALET DSSPPPYSSI TVEVPTTSDT EVYGEFYPVP PPYSVATSLP TYDEAEKAKA AAMAAAAAET SQRIQEEECP PRDDFSDADQ LRVGNDGIFM LAFFMAFIFN WLGFCLSFCI TNTIAGRYGA ICGFGLSLIK WILIVRFSDY FTGYFNGQYW LWWIFLVLGL LLFFRGFVNY LKVRNMSESM AAAHRTRYFF LL

Clonality

Polyclonal

Isotype

IgG

Host

Mouse

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2.

Purity/Purification

Affinity Purified
Purified by Protein A affinity chromatography.

Specificity

Recognizes human NDFIP2.

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

36,390 Da

Chromosome Location

Chromosome: 13; NC_000013.10 (80055259..80130212). Location: 13q31.1

OMIM

610041

3D Structure

ModBase 3D Structure for Q9NV92

Immunogen

Full length human NDFIP2, aa1-242 (AAH26126.1).

Product Categories/Family for anti-NDFIP2 antibody

Antibodies; Abs to Proteins

Applications Tested/Suitable for anti-NDFIP2 antibody

Western Blot (WB)

Application Notes for anti-NDFIP2 antibody

Suitable for use in Western Blot.

Other Notes

Small volumes of anti-NDFIP2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NDFIP2 purchase

MBS646335 is an antibody targeting NDFIP2. Also known as NDFIP2 (NEDD4 Family-interacting Protein 2, NEDD4 WW Domain-binding Protein 5A, Putative MAPK-activating Protein PM04/PM05/PM06/PM07, Putative NF-kappa-B-activating Protein 413, KIAA1165, N4WBP5A, FLJ25842). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for NDFIP2. It may not necessarily be applicable to this product.

NCBI GI #

239046743

NCBI GeneID

54602

NCBI Accession #

NP_001154879.1

UniProt Primary Accession #

Q9NV92

UniProt Secondary Accession #

Q7Z2H3; Q7Z428; Q8TAR3; Q9ULQ5

UniProt Related Accession #

Q9NV92

GenBank Nucleotide #

NM_001161407.1

NCBI Official Full Name

NEDD4 family-interacting protein 2 isoform 2

NCBI Official Synonym Full Names

Nedd4 family interacting protein 2

NCBI Official Symbol

NDFIP2

NCBI Official Synonym Symbols

N4WBP5A

UniProt Protein Name

NEDD4 family-interacting protein 2

UniProt Synonym Protein Names

NEDD4 WW domain-binding protein 5A; Putative MAPK-activating protein PM04/PM05/PM06/PM07; Putative NF-kappa-B-activating protein 413

Protein Family

NEDD4 family-interacting protein

UniProt Gene Name

NDFIP2

UniProt Synonym Gene Names

KIAA1165; N4WBP5A

UniProt Entry Name

NFIP2_HUMAN

NCBI Protein Information

NEDD4 family-interacting protein 2; NF-kappa-B-activating protein 413; NEDD4 WW domain-binding protein 5A; putative NF-kappa-B-activating protein 413; MAPK-activating protein PM04 PM05 PM06 PM07; putative MAPK-activating protein PM04/PM05/PM06/PM07

UniProt Comments for NDFIP2

Function: Activates HECT domain-containing E3 ubiquitin-protein ligases, including ITCH, NEDD4, NEDD4L, SMURF2, WWP1 and WWP2, and consequently modulates the stability of their targets. As a result, may control many cellular processes. Recruits ITCH, NEDD4 and SMURF2 to endosomal membranes. May modulate EGFR signaling. Ref.2 Ref.8 Ref.9

Subunit structure: Forms heterodimers with NDFIP1. Interacts with HECT domain-containing E3 ubiquitin-protein ligases, including NEDD4. Interacts with NEDD4L

By similarity. Interacts with PTEN. When phosphorylated at Tyr-167, interacts with SRC and LYN SH2 domain. May thus act as a scaffold that recruits SRC to NDFIP1, enhancing NDFIP1 phosphorylation. Interacts with SLC11A2/DMT1. May interact with phosphorylated EGFR. Ref.1 Ref.7 Ref.9

Subcellular location: Endosome membrane; Multi-pass membrane protein. Golgi apparatus membrane. Endosome › multivesicular body membrane Ref.1 Ref.7 Ref.9.

Tissue specificity: Expressed in brain, lung, heart, skeletal muscle, kidney, liver and placenta. Ref.1

Induction: By T-cell activation. Ref.1

Domain: The PY (WW-binding) motifs are required for E3 ubiquitin-protein ligase activation and for ubiquitination.

Post-translational modification: Ubiquitinated by NEDD4 and ITCH. Also ubiquitinated by NEDD4L. Ubiquitination by NEDD4 or NEDD4L does not affect turnover

By similarity. Ref.8Undergoes transient tyrosine-phosphorylation following EGF stimulation, most probably catalyzed by SRC. Also phosphorylated by LYN and FYN. Ref.9

Sequence caution: The sequence AAH21988.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH26126.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA91863.1 differs from that shown. Reason: Erroneous initiation.

Research Articles on NDFIP2

1. PTEN/Akt and MAP kinase signaling pathways are regulated by the ubiquitin ligase activators Ndfip1 and Ndfip2

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NDFIP2 antibody

Reference Product	PubMed Publications
NEDD4 antibody	>11 publications with NDFIP2 and NEDD4
ITCH antibody	>3 publications with NDFIP2 and ITCH
WWP2 antibody	>3 publications with NDFIP2 and WWP2
PTEN antibody	>1 publications with NDFIP2 and PTEN
LYN antibody	>1 publications with NDFIP2 and LYN
NEDD4L antibody	>1 publications with NDFIP2 and NEDD4L

Diseases associated with anti-NDFIP2 antibody

Disease Name	Pubmed Publications
Kidney Diseases Antibodies	>1 publications with NDFIP2 and Kidney Diseases
Inflammation Antibodies	>1 publications with NDFIP2 and Inflammation

Organs/Tissues associated with anti-NDFIP2 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>5 publications with NDFIP2 and Blood
Liver Antibodies	>2 publications with NDFIP2 and Liver
Heart Antibodies	>1 publications with NDFIP2 and Heart
Lung Antibodies	>1 publications with NDFIP2 and Lung
Ovary Antibodies	>1 publications with NDFIP2 and Ovary
Intestine Antibodies	>1 publications with NDFIP2 and Intestine
Kidney Antibodies	>1 publications with NDFIP2 and Kidney